Antioxidant activity of 2,4-di-tert-butylphenol isolated from plant growth promoting endophytic Streptomyces KCA-1
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Abstract
Endophytic Streptomyces produce vast amount of unique bioactive natural compounds, which they use to promote the growth and development of the host plant. In the current study, the putative endophytic Streptomyces strain KCA-1 was evaluated for its capacity to produce various hydrolytic enzymes and plant growth promoting (PGP) parameters. The five main antibiotics (Vancomycin (30µg), Gentamycin (10 mcg), Tetracycline (30 µg), Penicillin (6 µg), and Nalidixic acid (30 µg) were also tested for antibiotic sensitivity. Utilizing the DPPH, ABTS, and metal chelating assays, the antioxidant capacity of the bioactive compound 2,4-di-tert-butylphenol isolated from Streptomyces KCA-1 was assessed. Streptomyces KCA-1 produced ammonia and IAA at rates of 56.2 mg/ml and 47.7 µg/ml, respectively, according to quantitative analysis i01n UV-spectrophotometry. Additionally, by utilizing siderophore, amylase, protease, and cellulose, it was found to possess all additional PGP and enzyme characteristics. Tetracycline (30 µg) and penicillin (6 µg) were found to be resistant against the strain KCA-1, whereas vancomycin (30 µg), gentamycin (10 mcg), and nalidixic acid (30 µg) were found sensitive. The antioxidant activity of the pure bioactive compound 2,4-DTBP reached a maximum of 86.4%, 89.4%, and 85.0% of DPPH, ABTS scavenging, and metal chelating activity at 1000 µg/ml with an IC50 value of 60 µg/ml, 17 µg/ml, and 20 µg/ml, respectively. This work paints a comprehensive picture of endophytic Streptomyces KCA-1 as the possible source for 2,4-di-tert-butylphenol synthesis, which could be a promising potential source of drug in the pharmaceutical and agricultural sectors
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