Stomatal response of red chili (Capsicum annuum L.) due to water stress
Main Article Content
Abstract
The results showed that the water requirement of red chili (Capsicum annuum L.) plants varied depending on the Kc value in the growth phase. Under conditions of 30% ETc (T1), the water demand ranges from 0.63-2.4 mm/day; at 100% ETc (T2), the value of water requirements ranges from 2.12-8.00 mm/day; and at 150% ETc (T3), the value of water requirements ranges from 3.17-12.00 mm/day. The provision of different water requirements produces different stomatal responses. The highest number of stomata in T3 treatment was 16.25, and the least in T1 treatment was 10. The highest stomatal density in T3, T2, and T1 treatments were 185.22 mm-2, 136.78 mm-2, and 118.54 mm- 2, respectively. The largest porous area in the T2 treatment was 145.14 µm2, and the smallest in the T1 treatment was 113.17 µm2. Meanwhile, the stomata area and stomatal index in the three treatments were almost uniform, ranging from 577.44 µm2 to 629.36 µm2 for the stomata area and 0.17-0.2 for index stomata. It showed that the number of stomata and stomatal density of T1 and T2 treatments were significantly differed from T3 treatment. Meanwhile, the stomata area, porous area, and stomata index were not significantly differed. Plant morphological data were obtained by T3 treatment that gave the best results; production weight of 17.8 g, leaf area of 23.4 cm2, number of leaves 200, and plant height of 62 cm. The red chili plant in the greenhouse system was more adaptive to water drainage.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Ahanger, M. A. and Agarwal, R. M. (2017). Potassium up-regulates antioxidant metabolism and alleviates growth inhibition under water and osmotic stress in wheat (Triticum aestivum L). Protoplasma, 254:1471-1486.
Ahanger, M. A., Tomar, N. S., Tittal, M., Argal, S. and Agarwal, R. M. (2017). Plant growth under water/salt stress: ROS production; antioxidants and significance of added potassium under such conditions. Physiology and Molecular Biology of Plants, 23:731-744.
Alghory, A. and Yazar, A. (2019). Evaluation of crop water stress index and leaf water potential for deficit irrigation management of sprinkler-irrigated wheat. Irrigation Science, 37:61-77.
Almaroai, Y. A. and Eissa, M. A. (2020). Role of Marine Algae Extracts in Water Stress Resistance of Onion Under Semiarid Conditions. Journal of Soil Science and Plant Nutrition, 20:1092-1101.
Anjum, S. A., Ashraf, U., Zohaib, A., Tanveer, M., Naeem, M., Ali, I., Tabassum, T. and Nazir, U. (2017). Growth and Developmental Responses of Crop Plants Under Drought Stress: a Review. Zemdirbyste-Agriculture, 104:267-276.
Bangar, P., Chaudhury, A., Tiwari, B., Kumar, S., Kumari, R. and Bhat, K. V. (2019). Morphophysiological and biochemical response of mungbean [vigna radiata (L.) Wilczek] varieties at different developmental stages under drought stress. Turkish Journal of Biology, 43:58-69.
Bertolino, L. T., Caine, R. S. and Gray, J. E. (2019). Impact of stomatal density and morphology on water-use efficiency in a changing world. Frontiers in Plant Science, 10:https://doi.org/10.3389/fpls.2019.00225
Bhusal, N., Han, S. G. and Yoon, T. M. (2019). Impact of drought stress on photosynthetic response, leaf water potential, and stem sap flow in two cultivars of bi-leader apple trees (Malus × domestica Borkh.). Scientia Horticulturae, 246:535-543.
Bhusal, N., Kim, H. S., Han, S. G. and Yoon, T. M. (2020). Photosynthetic traits and plant–water relations of two apple cultivars grown as bi-leader trees under long-term waterlogging conditions. Environmental and Experimental Botany, 176:104-111.
Bhutta, M. A., Munir, S., Qureshi, M. K., Shahzad, A. N., Aslam, K., Manzoor, H. and Shabir, G. (2019). Correlation and Path Analysis Of Morphological Parameters Contributing To Yield In Rice (Oryza sativa) Under Drought Stress. Pakistan Journal of Botany, 51:81-88.
Bozkurt Çolak, Y., Yazar, A., Alghory, A. and Tekin, S. (2021). Evaluation of Crop Water Stress Index and Leaf Water Potential for Differentially Irrigated Quinoa with Surface and Subsurface Drip Systems. Irrigation Science, 39:81-100.
Descamps, C., Quinet, M., Baijot, A. and Jacquemart, A. L. (2018). Temperature and water stress affect plant–pollinator interactions in Borago officinalis (Boraginaceae). Ecology and Evolution, 8:3443-3456.
Dittberner, H., Korte, A., Mettler-Altmann, T., Weber, A. P. M., Monroe, G. and de Meaux, J. (2018). Natural variation in stomata size contributes to the local adaptation of water-use efficiency in Arabidopsis thaliana. Molecular Ecology, 27:4052-4065.
Gavili, S., Sanikhani, H., Kisi, O. and Mahmoudi, M. H. (2018). Evaluation of several soft computing methods in monthly evapotranspiration modelling. Meteorological Applications, 25:128-138.
Harrison, E. L., Arce Cubas, L., Gray, J. E. and Hepworth, C. (2020). The influence of stomatal morphology and distribution on photosynthetic gas exchange. Plant Journal, 101:768-779.
Hernandez-Santana, V., Fernández, J. E., Cuevas, M. V., Perez-Martin, A. and Diaz-Espejo, A. (2017). Photosynthetic limitations by water deficit: Effect on fruit and olive oil yield, leaf area and trunk diameter and its potential use to control vegetative growth of super-high density olive orchards. Agricultural Water Management, 184:9-18.
Hernandez-Santana, V., Perez-Arcoiza, A., Gomez-Jimenez, M. C. and Diaz-Espejo, A. (2021). Disentangling the link between leaf photosynthesis and turgor in fruit growth. Plant Journal, 107:1788-1801.
Hopkins, W. G. and Huner, N. P. (2018). Introduction to Plant Physiology (4th ed.). John Wiley & Sons, Inc.
Jafari, S., Hashemi Garmdareh, S. E. and Azadegan, B. (2019). Effects of drought stress on morphological, physiological, and biochemical characteristics of stock plant (Matthiola incana L.). Scientia Horticulturae, 253:128-133.
Kelebek, H., Sevindik, O., Uzlasir, T. and Selli, S. (2020). LC-DAD/ESI MS/MS characterization of fresh and cooked Capia and Aleppo red peppers (Capsicum annuum L.) phenolic profiles. European Food Research and Technology, 246:1971-1980.
Lahive, F., Hadley, P. and Daymond, A. J. (2019). The physiological responses of cacao to the environment and the implications for climate change resilience. A review. Agronomy for Sustainable Development, 39:https://doi.org/10.1007/s13593-018-0552-0
Liu, T. D., Zhang, X. W. and Xu, Y. (2020). Influence of red light on the expression of genes on stomatal formation in maize seedlings. Canadian Journal of Plant Science, 100:296-303.
Maylani, E. D., Yuniati, R. and Wardhana, W. (2020). The Effect of leaf surface character on the ability of water hyacinth, Eichhornia crassipes (Mart.) Solms. To transpire water. IOP Conference Series: Materials Science and Engineering, 902:https://doi.org/10.1088/1757-899X/902/1/012070
Mushtaq, R., Sharma, M. K., Ahmad, L., Krishna, B., Mushtaq, K. and Mir, J. I. (2020). Crop water requirement estimation using pan evaporimeter for high density apple plantation system in kashmir region of India. Journal of Agrometeorology, 22:86-88.
Nasir, M. W. and Toth, Z. (2021). Response of different potato genotypes to drought stress. Agriculture (Switzerland), 11:https://doi.org/10.3390/agriculture11080763
Omondi, J. O., Lazarovitch, N., Rachmilevitch, S., Yermiyahu, U. and Sperling, O. (2019). High Nitrogen Availability Limits Photosynthesis and Compromises Carbohydrate Allocation to Storage in Roots of Manihot esculenta Crantz. Frontiers in Plant Science, 10:1-9.
Parmar, S. H. and Tiwari, Dr. M. K. (2020). Crop water requirement (ETc) of Maize crop of Panchmahal Region of Gujarat. The Pharma Innovation, 9:281-286.
Pautov, A., Bauer, S., Ivanova, O., Krylova, E., Yakovleva, O., Sapach, Y. and Pautova, I. (2019). Influence of stomatal rings on movements of guard cells. Trees - Structure and Function, 33:1459-1474.
Pradhan, N., Singh, P., Dwivedi, P. and Pandey, D. K. (2020). Evaluation of sodium nitroprusside and putrescine on polyethylene glycol induced drought stress in Stevia rebaudiana Bertoni under in vitro condition. Industrial Crops and Products, 154:112754.
Pratama, A. B., Mangunwardoyo, W., Chandra, N. D., Napitupulu, T. P., Idris, I., Kanti, A., Ikhwani, A. Z. N., Sudiana, I. M. and Guswenrivo, I. (2021). Influence of AM fungi inoculation on Capsicum annuum L. plant grown in microwave-sterilized media. E3S Web of Conferences, 306:01057. https://doi.org/10.1051/e3sconf/202130601057
Rajanna, G. A., Dass, A. and Paramesha, V. (2018). Excess Water Stress: Effects on Crop and Soil, and Mitigation Strategies G. Popular Kheti, 6:48-53.
Raza, A., Razzaq, A., Mehmood, S. S., Zou, X., Zhang, X., Lv, Y. and Xu, J. (2019). Impact of climate change on crops adaptation and strategies to tackle its outcome: A review. Plants, 8:https://doi.org/10.3390/plants8020034
Saavedra, F., Jordan Peña, E., Schneider, M. and Naoki, K. (2020). Effects of environmental variables and foliar traits on the transpiration rate of cocoa (Theobroma cacao L.) under different cultivation systems. Agroforestry Systems, 94:2021-2031.
Suriani, S., Djaenuddin, N. and Talanca, A. H. (2019). Correlation of Stomata Density To Rust Severity on Some Accessions of Maize Germplasm. Jurnal Hama Dan Penyakit Tumbuhan Tropika, 18:95.
Time, A., Garrido, M. and Acevedo, E. (2018). Water relations and growth response to drought stress of prosopis tamarugo phil. A review. Journal of Soil Science and Plant Nutrition, 18:329-343.
Toscano, S., Ferrante, A. and Romano, D. (2019). Response of Mediterranean Ornamental Plants to Drought Stress. Horticulturae, 5:1-20.
Urban, J., Ingwers, M. W., McGuire, M. A. and Teskey, R. O. (2017). Increase in leaf temperature opens stomata and decouples net photosynthesis from stomatal conductance in Pinus taeda and Populus deltoides x nigra. Journal of Experimental Botany, 68:1757-1767.
Widiyono, W., Nugroho, S., Rachmat, A., Syarif, F., Lestari, P. and Hidayati, N. (2020). Drought tolerant screening of 20 indonesian sorghum genotypes through leaf water potential measurements under water stress. IOP Conference Series: Earth and Environmental Science, 439:https://doi.org/10.1088/1755-1315/439/1/012033
Zubaidi, S. L., Ortega-Martorell, S., Al-Bugharbee, H., Olier, I., Hashim, K. S., Gharghan, S. K., Kot, P. and Al-Khaddar, R. (2020). Urban water demand prediction for a city that suffers from climate change and population growth: Gauteng province case study. Water (Switzerland), 12:1-17.