Study on the growth patterns of transformed carrot hairy roots in an optimized system
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Abstract
Growth patterns of transformed carrot hairy roots in an optimized system of root induction were studied. Inoculation of mature carrot root sections was carried out using a mixture of four isolates of Agrobacterium rhizogenes that had been grown in three culture media (LB, YMA and modified medium) with 36 hours incubation time before inoculation. Results showed that most assays resulted in hairy root formation, although this was not equal. The source of the carrot was one of the most important factors, with mature, freshly harvested carrots showing better hairy root production. These roots had the ability to quickly form numerous lateral roots as well as a negative geotropic growth habit. Lateral roots were initiated from the both sides of the carrot discs and occurred from 8-10 days to 3-4 weeks. Root growth on MW medium followed a distinct pattern over 20 days and root elongation rates did not have a regular exponential trend over time.
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