Development of loop-mediated isothermal amplification (LAMP) for rapid detection of Lumpy Skin Disease Virus (LSDV) from cattle
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Abstract
Lumpy skin disease virus (LSDV) is an infectious double-stranded DNA virus of the genus Capripoxvirus that causes Lumpy skin disease (LSD), a serious problem in cattle livestock. LSD has the potential to spread rapidly and cause substantial economic damage. A key to preventing the spread of LSDV in cattle is rapid and accurate detection. Loop-mediated isothermal amplification (LAMP) assay is a novel technique that works by amplifying the specific target nucleic acid at a constant temperature, which is simple, fast, and easy to use in the field. This study developed a prototype LAMP assay to detect LSDV from various cattle samples, as compared to the conventional PCR assay. The results showed that, out of the total 30 cattle sampled with clinical signs of LSDV, 16.67% were positive for LSDV when detected by PCR assay, while 36.67% were positive for LSDV when detected by LAMP assay, indicating that the LAMP assay had a higher accuracy than conventional PCR assay. Moreover, the results showed that the LAMP assay had the same sensitivity as the conventional PCR assay, and both assays had highest detection rates when detected from nasal swab samples. Additionally, the nucleotide alignment was 100% identical to the LSDV strains from China, Hong Kong, and Vietnam. The LAMP assay can effectively detect the GPCR gene in LSDV from cattle samples, and it can be further used for developing a LAMP test kit to detect LSDV in the field.
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