Phytochemical screening, antioxidant activity and total phenolic content of methanolic extract of Phak Wan Ton (Crotalaria medicaginea Lam.)
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Abstract
The chlorophyll content of the methanolic extract of Crotalaria medicaginea without chlorophyll removal ranged from 0.50±0.03 to 1.24±0.06 mg/g extract, and with chlorophyll removal ranged from 0.09±0.01 to 0.18±0.00 mg/g extract. The highest total phenolic content using the Folin-Ciocalteu method was presented in the leaves extract without chlorophyll removal as 48.33±0.51 mg GEA/g extract. Moreover, the leaves extract without chlorophyll removal showed the lowest antioxidant activity with 50% inhibitory concentration (IC50) in DPPH radical scavenging assay at 344.76 µg/ml. Besides, the root extract exhibited rich potential for gram-positive bacteria, including Bacillus cereus, Bacillus subtilis, Kocuria rhizophila, Staphylococcus aureus and Staphylococcus epidermidis. The overall results showed that the extracts without chlorophyll removal had more efficiency than those with chlorophyll removal. Hence, the phytochemical screening was investigated only in the methanolic extract without chlorophyll removal. All extracts found alkaloids and coumarins, while tannins were found only in roots and did not display saponins. The current study suggested that the extracts of C. medicaginea Lam. gave the potential source of natural bioactive compounds.
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