Isolation and characterization of bacteria Causing bacterial wilt disease from fingerroot (Boesenbergia rotunda L.) in Phichit Province

Abstract

Samples of bacterial Wilt of fingerroot were collected from Phichit province. The Isolated bacteria were, irregular, whitish fluidal single colonies along with pinkish center on triphenyl tetrazolium chloride (TZC) were selected. On nutrient Agar (NA) white colony, entire margin. Total 26 bacteria isolates that fulfilled were further characterized by gram’s staining, physiological, biochemical testing. Results revealed that all of 26 bacterial isolates were Gram negative and used sugars dextrose lactose mannitol maltose and sorbitol which are R. solanacerum. But negative PCR detection with Psol_flic-F/ Psol_flic-R and 759/ 760/Nmutl21:1F/ Nmutl21:2F/ Nmutl23:AF/Nmutl22:InF/ Nmutl22:RR specific primer sets to R. solanacerum. And sequence comparative analysis of 16S rRNA gene from 5 isolates were subjected to BLAST analysis against the non-redundant nucleotide sequence collection at the National Center for Biotechnology Information (NCBI) grouped into Enterobacter spp.