Studies on Suitable Concentrations of Buserelin Acetate for Breeding and Embryonic Development of Rasbora borapetensis

Abstract

This study on the breeding of the blackline rasbora Rasbora borapetensis Smith, 1934 was conducted at the Ubon Ratchathani Inland Fisheries Research and Development Center from October 2021 to September 2022. The objective was to investigate the suitable concentrations of the synthetic hormone Buserelin acetate (Bus) for breeding performance and the embryonic development of early-stage larvae. Female broodfish, with an average weight of 2.53±0.15 g and an average length of 6.39±0.45 cm, were induced with Bus at four concentrations (0, 10, 20, and 30 µg/kg) combined with Domperidone (Dom) at 10 mg/kg following a completely randomized design (CRD) consisting of four treatments with three replications each. Male broodfish, averaging 1.44±1.78 g in weight and 5.20±0.45 cm in length, were injected with Bus at 10 µg/kg together with Dom at 10 mg/kg across all treatments. The results showed that Bus administration significantly affected ovulation, total egg production, fertilization rate, and hatching rate when compared with the distilled-water control (p<0.05). However, differences in Bus concentrations administered to females did not significantly influence larval survival (p>0.05). Embryonic development from fertilization to hatching required an average of 16 hours and 55 minutes at a water temperature of 27.0–30.0 °C. The findings indicate that a Bus concentration of 10 µg/kg combined with Dom at 10 mg/kg is the most suitable level for effective induction of blackline rasbora broodstock under the conditions of this study.

Keywords:   Rasbora borapetensis, breeding, Buserelin acetate, embryonic development