Impact of bulk and nano zinc oxide on histopathology of Labeo rohita
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Abstract
The UV-VIS analysis of bulk and nano zinc oxide was observed at 322 nm and 335nm. FT-IR spectral band was perceived at 401.12 cm-1 and 402 cm-1. XRD reveals that both bulk and nano ZnO were crystalline hexagonal wurtzite in structure The average was 65nm and 24nm. SEM photos revealed that chemically produced nano ZnO was spherical and evenly dispersed, in contrast to bulk zinc oxide which was rod-shaped. The purity of the particles is confirmed by the EDAX spectrum, which showed only Zn and O compound peaks for both bulk and chemically produced ZnO. The histological lesions such as basal hyperplasia, necrotic lamellae, a fusion of primary and secondary lamellae in the gill, melanomacrophages, pancreatic tissue, vacuolation, hepatic necrosis, apoptosis, oedema in the liver, Bowman's capsule extension and tubular distortion, glomerular enlargement and hemosiderin was detected in Labeo rohita kidney treated with bulk as well as nano zinc oxide when compared to the control.
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