Antibiotic biosynthesis gene of Bacillus velezensis as a bioagent for controlling blackleg and soft rot of potato
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Abstract
A total of 80 bacteria were isolated from the rhizospheres of healthy and asymptomatic potato plants. The bacterial isolates were screened by the co-inoculation method and it was found that strain CR74 had high antagonistic activities against bacterial blackleg and soft rot of potato caused by P. carotovorum subsp. brasiliense, D. dadantii, P. putida and B. altitudinis. Strain CR74 was identified as Bacillus velezensis through its 16S rRNA and gyrA genes. Strain CR74 inhibited synthesis of violacein and degraded the natural violacein of Chromobacterium violaceum ATCC 12472T. The antibiotic biosynthesis genes of strain CR74 were detected by sequence analysis. The results of PCR detection and sequencing showed the potential antibiotic genes of bacillomycin B, fengycin D, iturin A and surfactin. Therefore, B. velezensis strain CR74 is a potential biocontrol agents for the control of bacterial blackleg and soft rot on potato.
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