Growth response of Metarhizium anisopliae in indigenous media and efficacy to control rice black bug under greenhouse condition
Article Sidebar
Main Article Content
Abstract
Results revealed that the highest conidial count among media incubated at room temperature was in T7 (rice bran) with 13.1 x 109 conidia/ml which was significantly higher than other treatments. The same result was obtained in cultures under air conditioned environment wherein T7 (rice bran) had 15.5 x 109 conidial/ml. On substrates incubated at room temperature, the highest conidial count was observed in sorghum seeds (12.8 x 109) but not significantly different from rice bran (12.4 x 109), ground corn (11.6 x 109) and chipped camote tubers (11.1 x 109). Under air conditioned environment, the highest conidial count was observed in sorghum seeds (14.1 x 109) but not significantly different from rice bran (13.5 x 109). The highest mortality of Rice Black Bug (RBB) under greenhouse condition was observed in rice sprayed with M. anisopliae cultured in rice bran (70.00%) but not significantly different from palay seeds (65.00%), corn cob (58.33%) and ground corn (56.67%).
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Avin, F. (2019). Easy way to count spores and prepare spore suspension by Hemocytometer.
Aw, K. S. M and Hue, S. M. (2017). Mode of infection of Metarhizium spp. fungus and their potential as biological control agents. Journal Online of Fungi, 3:30.
Bena-Molaei, P., Talaei-Hassanloui, R., Askary, H. and Kharazi-Pakdel, A. (2011). Study on potential of some solid natural substances in production of Beauveria bassiana (Ascomycota, Cordycipitaceae) conidia. Journal of Entomological Society of Iran, 30:1-15.
Brooks A. J., de Muro M. A., Burree E., Moore D., Taylor M. A. and Wall R. (2004). Growth and pathogenicity of isolates of the fungus Metarhizium anisopliae against the parasitic mite, Psoroptes ovis: effects of temperature and formulation. Pest Management Science, 60:1043-1049.
Bugeme, D. M., Knapp, M., Boga, H. I., Wanjoya, A. K. and Maniania, N. K. (2009). Influence of temperature on virulence of fungal isolates of Metarhizium anisopliae and Beauveria bassiana to the two-spotted spider mite Tetranychus urticae. Mycopathologia, 167:221-227.
Chakraborty, M. and Budhwar, S. (2018). Nutritional and therapeutic value of rice bran. International Journal of Green and Herbal Chemistry, 7:451-461.
Cuaterno, W. R. (2011). Management of Malayan Rice Blackbug (Scotinophara Coarctata ) Using Biological Control Agent in the Island Provinces of the Philippines. Crop Protection Division Bureau of Plant Industry, Department of Agriculture, Philippines.
Ekesi, S., Maniania, N. K. and Ampong-Nyarko, F. (1999). Effect of temperature on germination, radial growth and virulence of Metarhizium anisopliae and Beauveria bassiana on Megalurothrips sjostedti. Biocontrol Science and Technology, 9:177-185.
EL Damir, M. (2006). Effect of growing media and water volume on conidial production of Beauveria bassiana and Metarhizium anisopliae. Journal of Biological Sciences, 6:269-274.
Fauzana, H., Arda, F., Nelvia. Rustam, R., and Puspita, F. (2020). Test on Several Concentrations Metarhizium anisopliae (Metsch) Sorokin in palm oil empty fruit bunch compost (metankos) to infecting Oryctes rhinoceros larvae. Journal of Physics: Conference Series, 1655:012021.
Gabarty, A., Salem, H. M., Fouda, M. A., Abas, A. A. and Ibrahim, A. A. (2013). Pathogencity induced by the entomopathogenic fungi Beauveria bassiana and Metarhizium anisopliae in Agrotisipsilon (Hufn.). J. of Radiation Research and Appl. Scienc, 7:95-100.
Gopal, M. A., Gupta and Thomas, G. V. (2005). Prospects of using Metarhizium anisopliae to check the breeding of insect pest Oryctes rhinoceros L. in coconut leaf vermicomposting sites J. Biosource Technology, 97:1801-1806.
Hallsworth, J. E. and Magan, N. (1999). Water and temperature relation of growth of the entomogenous fungi Beauveria bassiana, Metarhizium anisopliae and Paecilomyces farinosus. Journal of Invertebrate Pathology, 74:261-266.
Humpherson-Jones, F. M. and Phelps (1989). Climatic factors influencing spore production in Alternaria brassicae and Alternaria brassicicola, 114:449-458.
IRRI Reporter (1983). Black bug threatens rice in Palawan, 83:1-2.
Li, D. P. and Holdom, D. G. (1995). Effects of nutrients on colony formation, growth and sporulation of Metarhizium anisopliae (Deuteromycotina: Hyphomycetes) he. Journal of Invertebrate Pathology, 65:253-260.
Lim, G. S. (1975). Scotinophara coarctata F. sheltered within rice hills. Rice Entomology Newsletter, 3:5.
Okoye, J. I. and Obi, C. D. (2017). Chemical composition and sensory properties of wheat-African yam bean composite flour cookies. Discourse Journal of Agriculture and Food Sciences, 5:21-27.
Omodara, T. and Adebolu, T. (2014). Effect of formulated culture media on growth of some fungal species. International Journal of Botany and Research (IJBR), Vol. 4.
Parinduri, S., Saleh, A. and Suhanda, A. (2017) Test the effectiveness of prevention of hornbill larvae (Oryctes rhinoceros) using a chemical made from sipermetrin and the fungus Metarhizium anisopliae. Agro Estate Journal, 1:128-134.
Orquídea, P. G., Servando, H. Cantú-Bernal, N., Nayeli, C. C. and Víctor, E. A. (2021) Effect of physical and nutritional conditions on growth and conidial production and virulence of Beauveria sp. Against Lepidoptera and Coleoptera Pests, Southwestern Entomologist, 46:647-656. https://doi.org/10.3958/059.046.0306
Reissig, W. H., Heinrichs, E. A., Litsinger, J. A., Moody, K., Fiedler, L., Mew, T. W. and Barrion, A. T. (1986). Rice black bugs (Hemiptera: Pentatomidae). Illustrated guide to integrated pest management in rice in tropical Asia, pp.147-153. Los Baños, Laguna: International Rice Research Institute.
Shah, F. A., Wang, C. S. and Butt, T. M. (2005). Nutrition influences growth and virulence of the insect-pathogenic fungus Metarhizium anisopliae. FEMS Microbiology Letters, 251:259-266.
Sidana, A. and Farooq, U. (2014). Sugarcane Bagasse: A potential medium for fungal cultures. Hindawi Publishing Corporation Chinese Journal of Biology, 840505:1-5.
Sihombing, R. H., Oemry, S. and Lubis, L. (2014). Test the effectiveness of several entomopathogens in larvae of Oryctes rhinoceros L. (Coleoptera: Scarabaeidae) in the laboratory Online, Journal of Agrotechnology, 2:1300-1309.
Simbajon, S. (1992). Rice black bug situationer in Region IX Philippines. WESMARRDEC Newsl, 7:4-5.
Siswanto and Trisawa, I. M. (2017). Quality test and effectiveness of Metarhizium anisopliae isolate central kalimantan against Oryctes rhinoceros (Coleoptera: Scarabaeidae). Research Report. Plantation Research and Development Center. Bogor.
Tripathy, A. (2010). Yield evaluation of paddy straw mushrooms (Volvariella spp.) on various lignocellulosic wastes. International Journal of Applied Agricultural Research, pp.317-326.
Wicaksono, A. P., Abadi, A. L. and Afandhi, A. (2015). Test the effectiveness of the entomopathogenic fungus Beauveria bassiana (Bals.) Viullemin against the pupa of Bactroceracarambolae Drew & Hancock (Diptera: Tephritidae). Health Policy and Technology, 3:39-49.
Widiyanti, N. L. P. M. and Muyadihardja, S. (2004). Metarhizium anisopliae fungal toxicity test against Aedes aegypty mosquitoes. J. of Health Research and Development Media, 14:24-30.
