Possibility of split mushroom Schizophyllum commune by-product extracts as antimicrobial and antioxidant agent for aquaculture
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Abstract
Schizophyllum commune by product (SCB) extracted was investigated from different organic solvent having the different relative polarity consisting of hexane, ethyl acetate, dichloromethane, 2-butanol, ethanol, methanol and water as antimicrobial and antioxidant agent in order to added value of waste from agriculture. The antibacterial assay to pathogenic bacteria for economic fish culture Aeromonas hydrophila and Streptococcus agalactiae were tested by disc diffusion method. Antioxidant capacity was measured by DPPH assays. The result found that the inhibitory to pathogenic bacteria was not observed in all SCB extract compared to positive disc of commercial antibiotic (oxytetracyclin and norfloxacin) at the concentration of 20,000 ppm. While, the DPPH radical scavenging activity of each extract was significantly different (p <0.05). SCB extracted by methanol showed the highest activity (IC50=23 mg/mL) with no significant difference to SCB extracted by 2-butanol. The lowest level was observed in SCB extracted by ethanol and ethyl acetate (IC50=35 ug/mL). This study indicated that the different of organic solvent gave the different capacity of antioxidant level and SCB extract might be used as antioxidant agent in aquaculture.
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References
Ahmed, M., Abdullah, N., Shuib, A. S. and Razak, S. A. (2017). Influence of raw polysaccharide extract from mushroom stalk waste on growth and pH perturbation induced-stress in Nile tilapia. Aquaculture. 468:60-70.
Chang, S. T. (1999). World production of cultivated edible and medicinal mushrooms in 1997 with emphasis on Lentinus edodes (Berk.) Sing. in China. International Journal of Medicinal Mushrooms. 1:291-300.
Cheung, L. M., Cheung, P. C. K. and Ooi, V. E. C. (2003). Antioxidant activity and total phenolics of edible mushroom extracts. Food Chemistry. 81:249-255.
Collins, C. H. and Lyne, P. M. (1987). Microbiological Methods. Butter Worths and Co (Publishers) Ltd. London.
Doan, H. V., Hoseinifar, S. H., Tapingkae, W., Chitmanat, C. and Mekchay, S. (2017). Effects of Cordyceps militaris spent mushroom substrate on mucosal and serum immune parameters, disease resistance and growth performance of Nile tilapia (Oreochromis niloticus). Fish & Shellfish Immunology. 67:78-85.
Imtiaj, A., Jayasinghe, C, Lee, G. W., Kim, H. Y., Shim, M. J., Rho, H. S., Lee, H. S., Hur, H., Lee, M. W., Lee, U. Y. and Lee, T. S. (2008). Physicochemical requirement for the vegetative growth of Schizophyllum commune collected from different ecological origins. Mycobiology. 36:34-39.
Liu, F., Ooi, V. E. and Chang, S. T. (1997). Free radical scavenging activities of mushroom polysaccharide extracts. Life Sciences. 60:763-771.
Mau, J. L., Tsai, S. Y., Tseng, Y. H. and Huang, S. J. (2005). Antioxidant properties of hot water extracts from Ganoderma tsugae Murrill. Lebensmittel-Wissenschaft and Technologie. 38:589-597.
Mirfat, A. H. S., Noolidah, A. and Vikineswary, S. (2014). Antimicrobial activities of split gill mushroom (Schizophyllum commune Fr.). American Journal of Research Communication. 2:113-124.
Mirfat, A. H. S., Noolidah, A. and Vikineswary, S. (2010). Scavenging activity of Schizophyllum commune extracts and its correlation to total phenolic content. Journal of Tropical Agriculture and Food Science. 38:231-238.
Phromkunthong, W., Yokrat, S., Intasorn, S., Rattanakal, Y. and Nuntapong, N. (2014). Replacement of Grey Oyster Mushroom by-products (Pleurotussajor-caju (Fr.) Singers) for Fish Meal in Red Tilapia (Oreochromisniloticus x O. mossambicus) Practical Diet. Prawarun Agricultural Journal. 11:25-38.
Shimada, K., Fujikawa, K., Yahara, K. and Nakamura, T. (1992). Antioxidative properties of xanthan on the anti-oxidation of soybean oil in cyclodextrin emulsion. Journal of Agricultural and Food Chemistry. 40:945-948.
Srichanun.M., Nganwisuthiphan, T., Wanlem, S. and Rakkamon, T. (2017). Effects of different mushroom by-product types and levels on growth performance and survival rate in dietary of Nile tilapia (Oreochromis niloticus). International Journal of Agricultural Technology. 13:1093-1101.
Uluköy, G., Baba, E.and Öntas, C. (2016). Effect of Oyster Mushroom, Pleurotus ostreatus, Extract on Hemato‐Immunological Parameters of Rainbow Trout, Oncorhynchus mykiss. Journal of the World Aquaculture Society. 47:676-684.
