Germplasm collection and culture media optimization for cell lines of edible macrofungi from Bicol Natural Park, Region V, Philippines
Main Article Content
Abstract
In the part of Bicol National Park (BNP), twelve edible macrofungi were collected and identified from Sipocot, and Lupi, Camarines Sur. Two species namely, Auricularia auricula-judae and Schizophyllum commune are familiar and being consumed by the locals. Its mycelial cell lines were successfully rescued and subjected to media optimization. The longest mycelial increment and shortest day for full mycelial ramification of A. auricula-judae was significantly noted in Coconut Water Gulaman, with 30.47 mm having very thick mycelial growth within five days of incubation. On the other hand, fastest mycelial colonization of S. commune was observed in Corn Grit Sucrose Gulaman with a total increment of 23.36 mm which significantly colonized within seven days among evaluated medium. Additionally, it was noted that all the evaluated media were completely ramified by A. auricula-judae and S. commune. The ideal fruiting substrate based on the locally available and abundant materials in the region were being conducted at this moment.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Aldave, A. N., Beri, M.S., Cabrera, E. Q., Rodriguez, T. A., Abergos, K. N. B., Besmonte, C. B. and Bellere, A. D. (2021). Mycelial Growth Performance of Paddy Straw Mushroom (Volvariella Volvacea) in Banana Wastes. Agricultura CRI Journal, 1:1-9.
Cañal, A. A. A., Abogado, J. A. B. and Bellere, A. D. (2020). Mycelial Growth Performance of Pleurotus ostreatus and Volvariella volvacea in Common Kitchen Wastes. Asia Pacific Journal of Multidisciplinary Research, 8:78-85.
Chang, R. (1996). Functional properties of edible mushrooms. Nutrition Reviews, 54:S91.
Chang, S. T. and Wasser, S. P. (2012). The role of culinary-medicinal mushrooms on human welfare with a pyramid model for human health. International Journal of Medicinal Mushrooms, 14:95-134.
Chang, S. T. and Miles, P. G. (2004). Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact. CRC Press, Boca Raton, Fla, USA, 2nd edition.
DENR. (1992). National Parks of the Philippine City. Protected Areas and Wildlife Bureau, Quezon City, Philippines
Ergonul, P. G., Akata, I., Kalyoncu, F. and Ergonul, B. (2013). Fatty acid compositions of six wild edible mushroom species. The Scientific World Journal. Article ID 163964, 4 pages.
Dutta, S. (2013). Role of Mushroom as Nutriceutical an overview. International Journal of Pharma and Bio Sciences, 4: B59-B66.
Finimundy, T. C., Gambato, G. and Fontana, R. (2013). Aqueous extracts of Lentinula edodes and Pleurotus sajor-caju exhibit high antioxidant capability and promising in vitro antitumor activity. Nutrition Research, 33:76-84.
Guia, J. (2013). Scenic Forest in the Philippines. Retried from URL: https://www.vigattintourism.com/tourism/articles/Scienic-Forest-in-the-Philippines Date Retrieved: January 3, 2021.
Hanko, J. (2001). Mushroom Cultivation for People with Disabilities – A Training Manual. FAO. Retried from URL: https://www.fao.org/3/AB497E/ab497e07.htm#bm7.2 Date Accessed: February 27, 2023.
Hoa, H. T. and Wang, C. L. (2015). The Effects of Temperature and Nutritional Conditions on Mycelium Growth of Two Oyster Mushrooms (Pleurotus ostreatus and Pleurotus cystidiosus). Mycobiology, 43:14-23.
Heleno, S. A., Barros, L., Sousa, M. J., Martins, A. and Ferreira, I. C. F. R. (2010). Tocopherols composition of Portuguese Wild Mushroom with Antioxidant Capacity. Foodchemistry, 119:1443-1450.
Kalaw, S. P., Alfonso, D. O., Dulay, R. M. R., De Leon, A. M., Undan, J. Q., Undan, J. R. and Reyes, R. G. (2016). Optimization of culture conditions for secondary mycelial growth of wild macrofungi from selected areas in Central Luzon, Philippines. Current Research in Environmental & Applied Mycology, 6:277-287.
Kalaw, S. P., Dulay, R. M. R., Damaso, E. J., Ramos, J. C., Del Rosario, M. A. G., Abon, M. D., De Leon, A. M. and Reyes, R. G. (2022). Nutritional and physical requirements for mycelial growth and fruiting body production of six strains of Pleurotus djamor from Luzon Island, Philippines. Asian Journal of Agriculture and Biology, Online First. Retried from https://doi.org/10.35495/ajab.2021.05.233
Khan, M. M., Masiruddin, M., Rehman. and Khan, K. W. (2003). A study of chemical composition of Cocos nucifera L. (Coconut) water and its usefulness as rehydration fluid. Pakistan Journal Botany, 35:925-930.
Matilla, P., Konko, K. and Eurola, M. (2001). Contents of vitamins, mineral, elements, and some phenolic compounds in cultivated mushrooms. Journal of Agricultural and Food Chemistry, 49:2343-2348.
Nepal, S. (1995). Managing Protected Areas under Conditions of Conflict: Selected Case Studies from China, Myanmar, Nepal, Philippines, and Thailand pp.113.
Sahoo, A. K. (2014). Studies on Improvement of Bioefficiency of Straw mushroom (Volvariella spp.) in Orissa, pp.3.
Ullah, I., Ali, M. and Farooqi, A. (2010). Chemical and nutritional properties of some maize (Zea mays L.) varieties grown in NWFP, Pakistan. Pakistan Journal of Nutrition, 9:1113-1117.
USAID (2016). Philippines Biodiversity and Waters sheds improved for stronger economy and ecosystem resilience (B+WISER) Program. Retried from URL: https://www.usaid.gov/Philippines/energy-and-environment/bwiser Date Retrieved: January 3, 2021.
Yong, J. W. H., Ng., G. E. L. and Tan, S. N. (2009). The Chemical Composition and Biological Properties of Coconut (Cocos nucifera L.) Water, pp.5144-5164.
Yu, S., Weaver, V., Martin, K. and Cantorna, M. T. (2009). The effects of whole mushrooms during inflammation. BMC Immunology, 10:12.
Zhang, L., Fan, C., Liu, S., Zang, Z. and Jiao, L. (2011). Chemical composition and antitumor activity of polysaccharide from Inonotus obliquus. Journal of Medicinal Plants Research, 5:1251-1260.