Comparison of the bioactive potential of floral honey from the Western Ghats of India

Main Article Content

Hegde, S.
Sharathchandra, K.
Abhisheka, G.
Sridhar, K. R.

Abstract

The bioactive compounds and antioxidant activities of three floral honey samples produced by honey bees Apis dorsata, Apis indica and Trigona iridipennis in two locations in southwest India (the west coast and the Western Ghats) were investigated. Bioactive compounds are assessed, including total phenolics, tannins, flavonoids and vitamin C. The antioxidant potential evaluated included, total antioxidant activity, reducing power, ferrous ion-chelating capacity and free radical-scavenging activity. The current study showed a higher content of total phenolics and flavonoids in A. indica and T. iridipennis than Apis dorsata. Total phenolics, flavonoids and vitamin C in Western Ghats honey samples are higher than in many monofloral and multifloral honey samples evaluated in different parts of the world. In the present study, honey samples of A. dorsata and T. iridipennis possessed substantial antioxidant activity, proving their ethnic or traditional uses for therapeutic purposes. Further investigation on the beneficial properties of the honey samples for medicinal, bioactive and nutritional purposes from the Western Ghats and its vicinity is warranted

Article Details

How to Cite
Hegde, S., Sharathchandra, K., Abhisheka, G., & Sridhar, K. R. (2023). Comparison of the bioactive potential of floral honey from the Western Ghats of India. International Journal of Agricultural Technology, 19(3), 949–966. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/10277
Section
Original Study

References

Adebiyi, F. M., Akpan, I., Obiajunwa, E. L. and Olaniyi, H. B. (2004). Chemical physical characterization of Nigeria honey. Pakistan Journal of Nutrition, 3:278-81.

Adeyomoye, O. I., Olaniyan, O. T., Adewumi, N. and Anyakudo, M. M. (2021). Honey supplemented with vitamin C prevents dyslipidaemia and oxidative stress induced by exposure to lead acetate in Wistar rats. Indian Journal of Physiology and Pharmacology, 65:229-236.

Ahmed, S., Sulaiman, S. A., Baig, A. A., Ibraim, M., Liaqat, S. et al. (2018). Honey as a potential natural antioxidant medicine: An insight into its molecular mechanisms of action. Oxidative Medicine and Celular Longevity, 8367846. 10.1155/2018/8367846

Aliaño-González, M. J., Ferreiro-González, M., Espada-Bellido, E., Palma, M. and Barbero, G. F. (2019). A screening method based on visible-NIR spectroscopy for the identification and quantification of different adulterants in high-quality honey. Talanta, 203:235-241.

Aljadi, A. M. and Kamaruddin, M. Y. (2004). Evaluation of the phenolic contents and antioxidant capacities of two Malaysian floral honeys. Food Chemistry, 85:513-518.

Alvarez-Suarez, J. M., Tulipani, S., Romandini, S., Bertoli, E. and Battino, M. (2010). Contribution of honey in nutrition and human health: a review. Mediterranean Journal of Nutrition and Metabolism, 3:15-23.

Al-Waili, N. S. (2004). Effects of daily consumption of honey solution on hematological indices and blood levels of minerals and enzymes in normal individuals. Journal of Medicinal Food, 6:135-140.

Amabye, T. G. and Mekonen, F. (2016). Phytochemical and biochemical composition of wild honey a case study in eastern zone areas in Tigray, Ethiopia. Journal of Nutrition, Health & Food Engineering, 4:487-492. 10.15406/jnhfe.2016.04.00141

Amin, F. A. Z., Sabri, S., Mohammad, S. M., Ismail, M., Chan, K. W. et al. (2018). Therapeutic properties of stingless bee honey in comparison with European bee honey. Advances in Pharmaceutical Science, 6179596. 10.1155/2018/6179596

Amor, B. S., Mekious, S., Benfekih, L. A., Abdellattif, M. H., Boussebaa, W. et al. (2022). Phytochemical characterization and bioactivity of different honey samples collected in the Pre-Saharan region in Algeria. Life 12: 927. 10.3390/life12070927

Arawwawala, L. D. A. M. and Hewageegana, H. G. S. P. (2017). Health benefits and traditional uses of honey: A review. Journal of Apitherapy, 2:9-14.

Boussaid, A., Chouaibi, M., Rezig, L., Hellal, R., Donsì, F. et al. (2018). Physicochemical and bioactive properties of six honey samples from various floral origins from Tunisia. Arabian Journal of Chemistry, 11:265-274.

Brighenti, V., Licata, M., Pedrazzi, T., Maran, D., Bertelli, D. et al. (2019). Development of a new method for the analysis of cannabinoids in honey by means of high-performance liquid chromatography coupled with electrospray ionization-tandem mass spectrometry detection. Journal of Chromatography A, 1597:179-186.

Bueno-Costa, F. M., Zambiazi, R. C., Bohmer, B. W., Chaves, F. C., Silva, W. P. et al. (2016). Antibacterial and antioxidant activity of honeys from the state of Rio Grande do Sul, Brazil. LWT Food Science and Technology, 65:333-340.

Burns, R. (1971). Methods for estimation of tannins in grain sorghum. Agronomy Journal, 63:511-512.

Catelani, T. A., Bittar, D. B., Pezza, L. and Pezza, H. R. (2016). A rapid and eco-friendly method for determination of hydrolysable tannins and its application to honey samples. Food Analytical Methods, 9:m 2552–2559. 10.1007/s12161-016-0454-1

Chang, C., Yang, M., Wen, H. and Chern, J. (2002). Estimation of total flavonoid content in propolis by two complementary colorimetric methods. Journal of Food Drug Analysis, 10:178-182.

Chen, C. T., Chen, B. Y. and Nai, Y. S. (2019). Novel inspection of sugar residue and origin in honey based on the 13C/12C isotopic ratio and protein content. Journal of Food Drug Analysis, 27:175-183.

Chowdhury, M. (1999). Honey: is it worth rubbing it in? Journal of the Royal Society of Medicine, 92:663. 10.1177/014107689909201227

Chua, L. S., Rahaman, N. L. A., Adnan, N. A. and Tan, T. T. E. (2013). Antioxidant activity of three honey samples in relation with their biochemical components. Journal of Analytical Methods in Chemistry, 313798. 10.1155/2013/313798

Durović, V., Mandić, L., Mijatović, M., Miletić, N., Radovanović, M. et al. (2022). Comparative analysis of antibacterial and antioxidant activity of three different types of honey. Acta Agriculturae Serbica, 27:115-120.

Ferreira, I. C., Aires, E., Barreira, J. C. and Estevinho, L. M. (2009). Antioxidant activity of Portuguese honey samples: Different contributions of the entire honey and phenolic compounds. Food Chemistry, 114:1438-1443.

Gheldof, N., Wang, X. H. and Engeseth, N. J. (2002). Identification and quantification of antioxidant components of honeys from various floral sources. Journal of Agricultural and Food Chemistry, 50:5870-5877.

Haron, H., Talib, R. A., Subramaniam, P., Arifen, Z. N. Z. and Ibrahim, M. (2022). A comparison of chemical compositions in Kelulut honey from different regions. Malaysian Journal of Analytical Sciences, 26:447-456.

Havsteen, B. H. (2002). The biochemistry and medical significance of the flavonoids. Pharmacology & Therapeutics, 96:67-202.

Hegazi, A. G. and El-Hady, F. K. A. (2009). Influence of honey on the suppression of human low density lipoprotein (LDL) peroxidation (in vitro). Evidence-Based Complementary and Alternative Medicine, 6:113-121.

Hsu, C. L., Chen, W., Weng, Y. M. and Tseng, C. Y. (2003). Chemical composition, physical properties and antioxidant activities of yam flours as affected by different drying methods. Food Chemistry, 83:85-92.

Islam, M. R., Pervin, T., Hossain, H., Saha, B. and Hossain, S. J. (2017). Physicochemical and antioxidant properties of honeys from the Sundarbans Mangrove Forest of Bangladesh. Preventive Nutrition and Food Science 22, 335-344. 10.3746/pnf.2017.22.4.335

Júnior, D. S. P., Almeida, C. A., Santos, M. C. F., Fonseca, P. H. V., Menezes, E. V. et al. (2022). Antioxidant activities of some monofloral honey types produced across Minas Gerais (Brazil). PLoS ONE, 17:e0262038. 10.1371/journal.pone.0262038

Kaya, B. and Yildirim, A. (2021). Determination of the antioxidant, antimicrobial and anticancer properties of the honey phenolic extract of five different regions of Bingöl province. Journal of Food Science and Technology, 58:2420-2430.

Khalil, M. I. and Sulaiman, S. A. (2010). The potential role of honey and its polyphenols in preventing heart diseases: a review. African Journal of Traditional Complementary and Alternative Medicine, 7:315-321.

Khan, I. U., Dubey, W. and Gupta, V. (2014). Medicinal properties of honey: a review. International Journal of Pure and Applied Bioscience, 2:149-156.

Kwapong, P. K., Ilechie, A. A. and Kusi, R. (2013). Comparative antibacterial activity of stingless bee honey and standard antibiotics against common eye pathogens. Journal of Microbiology and Biotechnology Research, 3:9-15.

Leite, J. D. C., Trugo, L. C., Costa, L. S. M., Quinteiro, L. M. C., Barth, O. M. et al. (2000). Determination of oligosaccharides in Brazilian honeys of different botanical origin. Food Chemistry, 70:93-98.

Majtan, J., Sojka, M., Palenikova, H., Bucekova, M. and Majtan, V. (2020). Vitamin C enhances the antibacterial activity of honey against planktonic and biofilm-embedded bacteria. Molecules, 25:992; doi:10.3390/molecules25040992

Medhi, B., Puri, A., Upadhyay, S. and Kaman, L. (2008). Topical application of honey in the treatment of wound healing: a meta-analysis. JK Science 10: 166-169.

Mei, S. J., Nordin, M. M. and Sani, N. A. (2010). Fructooligosaccharides in honey and effects of honey on growth of Bifidobacterium longum BB 536. International Food Research Journal, 17:557-561.

Moniruzzaman, M., Yung An, C., Rao, P. V., Hawlader, M. N., Azlan, S. A. et al. (2014). Identification of phenolic acids and flavonoids in monofloral honey from Bangladesh by high performance liquid chromatography: Determination of antioxidant capacity. BioMed Research International, 737490. 10.1155/2014/737490

Moundo, M. A., Padila-Zakour, O. I. and Worobo, R. W. (2001). Antimicrobial activity of honey against food pathogens and food spoilage microorganisms. New York State Agricultural Experimental Station, 1:61-71.

Nedić, N., Nešović, M., Radišić, P., Gašić, U, Baošić, R. et al. (2022). Polyphenolic and chemical profiles of honey from the Tara Mountain in Serbia. Front. Nutrition, 9:941463. 10.3389/fnut.2022.941463

Oriolowo, O. B., John, O. J., Abubakar, D. S., Jonah, T. M. and Ismaila, D. (2019). Anti-nutritional composition of honey samples from four Northern states of Nigeria. Nigerian Journal of Basic and Applied Science, 27:32-38.

Otero, M. C. B. and Bernolo, L. (2020). Honey as Functional Food and Prospects in Natural Honey Production. In: Egbuna, C. and Dable-Tapas, G. (eds.). Functional Foods and Nutraceuticals, Springer Nature Switzerland AG, pp. 197-210.

Oyaizu, M. (1986). Studies on products of browning reactions: antioxidative activities of products of browning reaction prepared from glucosamine. Japanese Journal of Nutrition, 44:307-315.

Pham, T. N., Nguyen, T. V., Le, D. T., Diep, L. M. N., Nguyen, K. N. et al. (2022). Phenolic profiles, antioxidant, antibacterial activities and nutritional value of Vietnamese honey from different botanical and geographical sources. AgriEngineering, 4:1116-1138.

Prieto, P., Pineda, M. and Aguilar, M. (1999). Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of vitamin E. Anal Biochem, 269:337-341.

Puścion-Jakubik, A., Borawska, M. H. and Socha, K. (2020). Modern methods for assessing the quality of bee honey and botanical origin identification. Foods, 9:1028. 10.3390/foods9081028

Roe, J. H. (1954). Chemical determination of ascorbic, dehydroascorbic and diketogluconic acids. In: Glick, D. (ed.). Methods of biochemical analysis, Volume 1. InterScience Publishers, New York, pp.115-139.

Rosset, J., Bärlocher, F. and Oertli, J. J. (1982). Decomposition of conifer needles and deciduous leaves in two Black Forest and two Swiss Jura streams. International Revew Gesamten Hydrobiollogie, 67:695-711.

Sagona, S., Minieri, S., Coppola, F., Gatta, D., Casini, L. et al. (2021). Effects of chestnut hydrolysable tannin enrichment in the artificial diet of forager bees, Apis mellifera, Journal of Apicultural Research, 60(3). 10.1080/00218839.2021.1960744

Salomon, V. M., Brodkiewicz, I. Y., Gennari, G. P., Maldonado, L. M., Romero, C. M. and Vera, N. R. (2022). Argentine stingless bee honey: bioactive compounds and health-promoting properties. Natural Resources for Human Health, 2:236-245. 10.53365/nrfhh/144727

Sawicki, T., Bączek, N. and Starowicz, M. (2020). Characterisation of the total phenolic, vitamins C and E content and antioxidant properties of the beebread and honey from the same batch. Czech Journal of Food Sciences, 38:158-163.

Sewllam, T., Miyanaga, N., Onozawa, M., Hattori, K., Kawai, K. et al. (2003). Antineoplastic activity of honey in an experimental bladder cancer implantation model: in vivo and in vitro studies. International Journal of Urology, 10:213-219.

Shubharani, R., Anita, M., Mahesh, M. and Sivaram, F. (2013). Antioxidant and Antibacterial Activity of Different Floral Honeys from Western Ghats of Karnataka. International Journal of Pharmaceutical Science Review and Research, 20:104-108.

Singh, R. P., Murthy, C. K. N. and Jayaprakasha, G. K. (2002). Studies on antioxidant activity of pomegranate (Punica granatum) peel and seed extracts using in vitro methods. Journal of Agricultural and Food Chemistry, 50:81-86.

StatSoft (2008). Statistica Version # 8. StatSoft Inc, Tulsa, Oklahoma.

Stobener, A., Naefken, U., Kleber, J. and Liese, A. (2019). Determination of trace amounts with ATR FTIR spectroscopy and chemometrics: 5-(hydroxymethyl) furfural in honey. Talanta, 204:1-5.

Thomas, K. J., Nicoll, J. P. and Coleman, P. (2001). Use and expenditure on complementary medicine in England: a population based survey. Complementary Therapies in Medicine, 9:2-11.

Tonks, A. J., Cooper, R. A., Jones, K. P., Blair, S., Parton, J. and Tonks, A. (2003). Honey stimulates inflammatory cytokine production from monocytes. Cytokine, 21:242-247.

Viteri, R., Zacconi, F., Montenegro, G. and Giordano, A. (2021). Bioactive compounds in Apis mellifera monofloral honeys. Journal of Food Science, 2021:1-31. 10.1111/1750-3841.15706

Wang, S., Liu, J., Yong, W., Chen, O., Zhang, L. et al. (2015). A direct competitive assay-based aptasensor for sensitive determination of tetracycline residue in honey. Talanta, 131:562-569.

Wilkinson, J. M. and Simpson, M. D. (2001). High use of complementary therapies in a New South Wales rural community. Australian Journal of Rural Health, 9:166-171.

Yelin, A. and Kuntadi (2019). Phytochemical identification of honey from several regions in Java and Sumbawa. American Institute of Physics Conference Proceedings, 2120:080024. 10.1063/1.5115762

Zamudio, F., Kujawska, M. and Hilgert, N. I. (2010). Honey as medicinal and food resource. Comparison between polish and multiethnic settlements of the Atlantic forest, Misiones, Argentina. The Open Complementary Medicine Journal, 2:58-73.