Physicochemical properties of caramel toffee products supplemented with whey protein and rice bran hydrolysate

Main Article Content

Khongla, C.
Plangklang, T.
Pimsuwan, C.
Jitjangwat, N.
Musika, S.
Mangkalanan, S.
Kupradit, C.
Ranok, A.
Bunsroem, K.
Sangsawad, P.

Abstract

A caramel toffee product (CTP) supplemented with whey protein concentrate (WPC) and rice bran hydrolysate (RBH) produced in pilot scale process was investigated. Three recipes of CTPs were prepared using different WPC levels (0.0, 2.0, and 5.0%). The sensory attributes were then evaluated using a 9-point hedonic scale. The results showed that the addition of WPC at 2.0% had no negative effect on all sensory attributes, with an overall acceptability score of 7.73 (Like moderately to like very much), when compared with the CTP without the addition of WPC (control). Therefore, 2.0% WPC was selected for further production of CTPs supplemented with different RBH concentrations (0.0-2.0%). α-Amino contents and antioxidant activities of CTPs were determined. The CTP supplemented with 2%RBH plus 2.0% WPC (CTP_2% RBH_WPC) exhibited the highest α-amino content (0.47 mg Leucine Eq./g sample), ABTS radical scavenging activity (0.88 mg Trolox Eq./g sample) and ferric reducing antioxidant power (0.52 mg Trolox Eq./g sample). However, the CTP_2% RBH_WPC had lower acceptability by panelists than that of control (0% RBH). Therefore, the CTP_2% RBH_WPC was selected for further development by adding vanilla and caramel flavors. The aw, color, chemical compositions, sensory evaluation (n=311), and microbial load of flavored CTP were then determined. The aw, moisture content, and L*, a*, and b* values were 0.45, 3.90%, 57.90, 12.47, and 21.62, respectively. Protein, fat, ash, and carbohydrate of the flavored CTP were 2.60, 15.10, 1.12, and 77.33%, respectively. The overall acceptability score of the flavored CTP was 7.57 and the microbial load were within the Thai community product standard of Toffee. These results suggested that WPC and RBH have potential as nutritive and antioxidative substance supplementation in the caramel toffee product.

Article Details

How to Cite
Khongla, C., Plangklang, T., Pimsuwan, C., Jitjangwat, N., Musika, S., Mangkalanan, S., Kupradit, C., Ranok, A., Bunsroem, K., & Sangsawad, P. (2023). Physicochemical properties of caramel toffee products supplemented with whey protein and rice bran hydrolysate. International Journal of Agricultural Technology, 19(3), 1011–1028. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/10306
Section
Original Study

References

Adler-Nissen, J. (1979). Determination of the degree of hydrolysis of food protein hydrolyzates by trinitrobenzenesulfonic acid. Journal of Agricultural and Food Chemistry, 27:1256-1262.

AOAC (2000). Official methods of analysis (17th ed.). Gaithersberg, Maryland: Association of Official Chemists.

Auwal, S. M., Zarei, M., Abdul-hamid, A. and Saari, N. (2017). Response surface optimisation for the production of antioxidant hydrolysates from Stone fish protein using bromelain. Evidence-Based Complementary and Alternative Medicine, 2017:4765463.

Benzie, I. F. F. and Strain, J. J. (1996). The ferric reducing ability of plasma (FRAP) as a measure of ''antioxidant power'': The FRAP assay. Analytical Biochemistry, 239:70-76.

Bhokre, C. K., Rodge, A. B., Ghatge, P. U. and Chilkawar, P. M. (2010). Preparation and evaluation of banana toffee. Beverage & Food World, 37:50-51.

Chanput, W., Theerakulkait, C. and Nakai, S. (2009). Antioxidative properties of partially purified barley hordein, rice bran protein fractions and their hydrolysates. Journal of Cereal Science, 49:422-428.

Chen, Z., Li, W., Kumar, R., Cong, S., Xudong, W., Yue, G., Chunli, C., Xu, L. and Chen, H. (2018). Bioactive peptide with antioxidant and anticancer activities from black Soybean [Glycine max (L.) Merr.] byproduct: isolation, identification and molecular docking study. European Food Research and Technology, 245:677-689.

Chen, K., Zhao, J., Shi, X., Abdul, Q. and Jiang, Z. (2019). Characterization and antioxidant activity of products derived from xylose–bovine casein hydrolysate Maillard reaction: Impact of reaction time. Foods, 8:242.

Cho, S. J. (2020). Changes in the antioxidant properties of rice bran protein isolate upon simulated gastrointestinal digestion. Lebensmittel-Wissenschaft und-Technologie, 126:109206.

Corradini, M. G. (2019). Synthetic Food Colors. In: Melton L, Shaidi F, Varelis P eds. Encyclopedia of food chemistry, New York, Elsevier, pp.291-296.

Ergun, R., Lietha, R. and Hartel, R. W. (2010). Moisture and shelf life in sugar confections. Critical Reviews in Food Science and Nutrition, 50:162-192.

Gunathilake, K. D. P. P., Jaavidh, M. A. M., Perera, G. R. P. K., Thilakahewa, C. and Kumara, A. A. N. (2010). Value added products from Dikiri coconuts: preparation, compositional and sensory qualities. Cord, 26:18-23.

Gehlot, R., Siddiqui, S. and Grewal, R. (2018). Development of mixed fruit toffee from guava and mango blends and its quality evaluation during storage. International Journal of Chemical Studies, 6:1330-1332.

Jafary, S. H., Ahmad, R. S., Hussain, M. B., Rehman, T., Majeed, M., Khan, M. U. and Shariati, M. A. (2018). Investigation of changes in antioxidant activities of caramelization products under various time regimes and pH ranges. Carpathian Journal of food science and Technology, 10:116-128.

Jay, J. M., Loessner, M. J. and Golden, D. A. (2005). Protection of Foods by Drying. Modern food microbiology. Food Science Text Series, Boston, Springer, pp. 443-456.

Jiang, Z., Rai, D. K. O'Connor, P. M. and Brodkorb, A. (2013). Heat-induced Maillard reaction of the tripeptide IPP and ribose: Structural characterization and implication on bioactivity. Food Research International, 50:266-274.

Khapre, A. P. (2010). Standardization of technology for development of guava – soybean toffee as a protein enriched product. Food Science Research Journal, 1:154-156.

Khongla, C., Musika, S., Sangsawad, P., Srithamma, L., Kiatbenjakul, P., and Tanamool, V. (2022a). Antioxidant activity of Jasmine rice bran hydrolysate and its application in broken rice beverages. Suranaree Journal of Science and Technology, 29:020013(1-10).

Khongla, C., Chuaingan J., Siadkhunthod, T., Somnam, P., Musika, S., Sangsawad, P. (2022b). Physicochemical properties of rice bran hydrolysate prepared in a pilot scale process and its application in milk tablets. Trends in Sciences. 19:2316-2316.

Kohinkar, S. N., Chavan, U. D., Pawar, V. D. and Amarowicz, R. (2014). Studies on preparation of mixed fruit toffee from Fig and Guava fruits. J Food Sci Technol. 51:2204-2209.

Liao, X., Zhu, Z., Wu, S., Chen, M., Huang, R., Wang, J., Wu, Q. and Ding, Y. (2020). Preparation of antioxidant protein hydrolysates from Pleurotus geesteranus and their protective effects on H2O2 oxidative damaged PC12 cells. Molecules, 25:5408.

Lobo, V., Patil, A., Phatak, A. and Chandra, N (2010). Free radicals, antioxidants and functional foods: Impact on human health. Pharmacognosy Reviews, 4:118-126.

Lu, X., Zhang, L. Sun, Q., Song, G. and Huang, J. (2019). Extraction, identification and structure-activity relationship of antioxidant peptides from Sesame (Sesamum indicum L.) protein hydrolysate. Food Research International, 116:707-716.

Mazur, L., Gubsky, S., Dorohovych, A. and Labazov, M. (2018). Antioxidant properties of candy caramel with plant Extracts. Ukrainian Food Journal, 7:7-21.

Mendenhall, H. and Hartel, R. W. (2016). Protein content affects caramel processing and properties. Journal of Food Engineering, 186:58-68.

Minj, S. and Anand, S. (2020). Whey Proteins and its derivatives: Bioactivity, functionality, and current applications. Dairy, 1:233-258.

Mohos, F. A. (2010). Chemical operations (inversion and caramelization), ripening and complex operations. In: Mohos, FA ed. Confectionery and chocolate engineering: Principles and applications, Iowa, Wiley-Blackwell Publishing, pp.502-504.

Nooshkam, M., Varidi, M. and Bashash, M. (2019). The Maillard reaction products as food-born antioxidant and antibrowning agents in model and real food systems. Food Chemistry, 275:644-660.

Sangwan, S. and Seth, R. (2021). Whey protein supplement: an exclusive food or need of the hour: Review. Annual Research & Review in Biology, 36:110-119.

Sarmadi, B. H. and Ismail, A. (2010). Antioxidative peptides from food proteins: A review. Peptides, 31:1949-1956.

Smithers, G. W. (2008). Whey and whey proteins—From ‘gutter-to-gold’. International Dairy Journal, 18:695-704.

Stojanovska, S., Gruevska, N., Tomovska, J., Tasevska, J., Krstanovski, A. and Menkovska, M. (2017). Maillard reaction and lactose structural changes during milk processing. Chemistry Research Journal, 2:139-145.

Supaking, J. (2019). Development of Nipa palm fruit toffee product. Journal of Food Technology, 14:48-57.

Thamnarathip, P., Jangchud, K., Nitisinprasert, S. and Vardhanabhuti, B. (2016). Identification of peptide molecular weight from rice bran protein hydrolysate with high antioxidant activity. Journal of Cereal Science, 69:329-335.

Vhangani, L. N. and Van Wyk, J. (2013). Antioxidant activity of Maillard reaction products (MRPs) derived from fructose-lysine and ribose-lysine model systems. Food Chemistry, 137:92-98.

Wang, H., Yang, J., Yang, M. and Ji, W. (2019). Antioxidant activity of Maillard reaction products from a Yak casein-glucose model system. International Dairy Journal, 91:55-63.

Wattanasiritham, L., Theerakulkait, C., Wickramasekara, S., Maier, C. S. and Stevens, J. F. (2016). Isolation and identification of antioxidant peptides from enzymatically hydrolyzed Rice bran protein. Food Chemistry, 192:156-162.

Wiriyaphan, C., Chitsomboon, B. and Yongsawadigul, J. (2012). Antioxidant activity of protein hydrolysates derived from threadfin bream surimi byproducts. Food Chemistry, 132:104-111.

Xiang, J., Liu, F., Wang, B., Chen, L., Liu, W. and Tan, S. (2021). A literature review on Maillard reaction based on milk proteins and carbohydrates in food and pharmaceutical products: Advantages, disadvantages, and avoidance strategies. Foods, 10:1998.

Yang, J., Hu, L., Cai, T., Chen, Q. and Ma, Q. (2018). Purification and identification of two novel antioxidant peptides from perilla (Perilla frutescens L. Britton) seed protein hydrolysates. PLoS ONE, 13: e0200021.

Zaky, A. A., Chen, Z., Liu, Y., Li, S. and Jia, Y. (2019). Preparation and assessment of bioactive extracts having antioxidant activity from rice bran protein hydrolysates. Journal of Food Measurement and Characterization, 13:2542-2548.