Effects of tropical forage species on Butyrivibrio fibrisolvens, Fibrobacter succinogenes and total bacteria population in goat rumen using real-time PCR techniques

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Published: Dec 15, 2018
Keywords:
forage species Butyrivibrio fibrisolvens Fibrobacter succinogenes total bacteria population

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Thongruang, S.
Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand, Faculty of Animal Sciences and Agricultural Technology, Silpakorn University, Phetchaburi, Thailand.
Paengkoum, P.
Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Suksombat, W.
Institute of Agricultural Technology, Suranaree University of Technology, Nakhon Ratchasima, Thailand.
Bureenok, S.
Faculty of Sciences and Liberal Arts, Rajamangala University of Technology Isan, Nakhon Ratchasima, Thailand.

Abstract

Three ruminally fistulated crossbred Saanen male goats (approximately 33 ± 3.0 kg BW) were used to determine the effect of 6 tropical forage diets on B. fibrisolvens, F. succinogenes and total bacteria population in goat rumen using real-time PCR techniques. The results showed that in grass experiment, three grass species including Purple Guinea (Panicum maximum TD58), Chinese Pennisetum (Pennisetum purpureum x Pennisetum alopecuroides) and Napier Pak Chong 1 (Pennisetum purpureum x Pennisetum americanum) were not significantly (p>0.05) different in the population of B. fibrisolvens (1.51-2.97 x 106 copies/ml), F. succinogenes (4.21-5.98 x 106 copies/ml) and total bacteria (4.08-6.58 x 1010 copies/ml) in goat rumen at 0, 2, 4 and 6 h after feeding. Similarly, legumes experiment, the population of ruminal B. fibrisolvens (1.31-3.01 x 106 copies/ml), F.succinogenes (4.01-5.73 x 106 copies/ml) and total bacteria (3.12-5.40 x 1010 copies/ml) of goats were not significantly (p>0.05) different between three legume species including Hamata (Stylosanthes hamate), Hedge Lucern (Desmanthus virgatus) and Leucaena (Leucaena leucocephala) at 0, 2, 4 and 6 h after feeding.

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Thongruang, S., Paengkoum, P., Suksombat, W., & Bureenok, S. (2018). Effects of tropical forage species on Butyrivibrio fibrisolvens, Fibrobacter succinogenes and total bacteria population in goat rumen using real-time PCR techniques. International Journal of Agricultural Technology, 14(7), 2125–2136. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/10327
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Vol. 14 No. 7 (2018): December
Section
Original Study
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References

AOAC (1985). Official Methods of Analysis Association of Official Chemists. Washington, D.C. pp. 771.

AOAC (1995). Official Methods of Analysis. 16th ed. Association of Official Anlytical Chemists, Arlington, VA, USA. pp. 1110.

Bryant, M. P. (1959). Bacterial species of the rumen. Bacterial Reviews. 23:125-153.

Collado, M. C. and Sanz, Y. (2006). Quantification of mucosa-adhered microbiota of lambs and calves by the use of culture methods and fluorescent in situ hybridization coupled with flow cytometry techniques. Veterinary Microbiology. 121:299-306.

Denman, S. E. and McSweeney, C. S. (2006). Development of a real-time PCR assay for monitoring anaerobic fungi and cellulolytic bacterial populations within the rumen. FEMS Microbiology Ecology. 53:572-582.

Dewhurst, R. J., Fisher, W. J., Tweed, J. K. S. and Wilkins, R. J. (2003). Comparison of grass and legume silages for milk production. 1. Production responses with different levels of concentrate. Journal of Dairy Science. 86:2598-2611.

Folch, J., Lees, M. and Sloane-Stanley, G. H. (1957). A simple method for the isolation and purifivation of total lipids from animal tissues. The Journal of Biological Chemistry. 226:459-509.

Forster, R. J., Gong, J. and Teather, R. M. (1997). Group-specific 16S rRNA hybridization probes for determinative and community structure studies of Butyrivibrio fibrisolvens in the rumen. Applied and Environmental Microbiology. 63:1256-1260.

Guo, T. J., Wang, J. Q., Bu, D. P., Liu, K. L., Wang, J. P., Li, D., Luan, S. Y. and Huo, X. K. (2010). Evaluation of the microbial population in ruminal fluid using real time PCR in steers treated with virginiamycin. Czech Journal of Animal Sciences. 55:276-285.

Hungate, R. E. (1966). The rumen and its microbes. Academic Press, New York, pp. 53.

Keawthong, K. (2002). Napier Pak Chong 1 Planting Handbook. Nakhon Ratchasima Animal Feed Research and Development Center, Animal Nutrition Division, Department of Livestock Development, Ministry of Agriculture and Cooperatives, Thailand. pp. 24.

Khaing, K. T., Loh, T. C., Ghizan, S., Jahromi, M. F., Halim, R. A. and Samsudin, A. A. (2016). Profiling of rumen fermentation and microbial population changes in goats fed with Napier grass supplemented with whole corn plant silage. Asian Journal of Animal Sciences. 10:1-14.

Khan, N. A., Forooq, M. W., Ali, M., Suleman, M., Ahmad, N., Sulaiman, S. M., Cone, J. W. and Hendriks, W. H. (2015). Effect of species and harves maturity on the fatty acids profile of tropical forages. The Journal of Animal and Plant Sciences. 25:739-746.

Kobayashi, Y., Forster, R. J. and Teather, R. M. (2000). Development of a competitive polymerase chain reaction assay for the ruminant bacterium Butyrivibrio fibrisolvens OB156 and its use for tracking an OB156 derived recombinant. FEMS Microbiology Letters. 188:185-190.

Krebs, G., Leng, R. A. (1984). The effect of supplementation with molasses/urea blocks on ruminal digestion. Proceeding of Australian Production. pp. 704.

Laso, N., Brugue, E., Vidal, J., Ros, E., Arnaiz, JA., Carne, X., Vidal, S., Mas, S., Deulofeu, R. and Lafuente, A. (2007). Effects of milk supplementation with conjugated linoleic acid (isomer cis-9, trans-11 and trans-10, cis-12) on body composition and metabolic syndrome components. British Journal of Nutrition. 98:860-867.

Leng, R. A. (1990). Factors affecting the utilization of poor quality forages by ruminants particularly under tropical conditions. Nutrition of Research Review. 3:277-303.

Leser, T. D., Boye, M. and Hendriksen, N. B. (1995). Survival and activity of a Pseudomonas sp. Strain B13 (FR1) in a marine microcosm determined by quantitative PCR and an rRNA-targeting probe and its effect on the indigenous bacterioplankton. Appl Environ Microbiol. 61:1201-1207.

McSweeney, C. S. and Denman, E. (2007). Effect of sulfur supplements on cellulolytic rumen micro-organisms and microbial protein synthesis in cattle fed a high fiber diet. Appl Microbial. 103:1757-1765.

Metcalfe, L. D., Schmitz, A. A. and Pelka, J. R. (1966). Rapid preparation of fatty acid esters from lipids for gas chromatographic analysis. Analytical Chemistry. 38:514-515.

Metzler-Zebeli, B. U., Schmitz-Esser, S., Klevenhusen, F., Podstatzky-Lichtenstein, L., Wagner, M. and Zebeli, Q. (2013). Grain-rich diets differently alter ruminal and colonic abundance of microbial populations and lipopolysaccharide in goats. Anaerobe. 20:65-73.

O'Shea, M., Devery, R., Lawless, F., Murphy, J. and Stanton, C. (2000). Milk fat conjugated linoleic acid (CLA) inhibits growth of human mammary MCF-7 cancer cells. Anticancer Research. 20:3591-3601.

O'Shea, M., Lawless, F., Stanton, C. and Devery, R. (1998). Conjugated linoleic acid in bovine milk fat: a food-based approach to cancer chemoprevention. Trends in Food Science and Technology. 9:192-196.

Ostrowska, E., Dunshea, FR., Muralitharan, M. and Cross, R. F. (2000). Comparison of silver-ion high-performance liquid chromatographic quantification of free and methylated conjugated linoleic acids. Lipids. 35:1147-1153.

Paengkoum, P. and Traiyakun, S. (2011). Ruminal and intestinal digestibility of leucaena (Leucaena leucocephala) and Jack fruit (Artocarpus heterophyllus) foliage using In sacco and three-step techniques. Research Journal of Applied Sciences. 6:88-91.

Reilly, K. and Attwood, G. T. (1998). Detection of Clostridium proteoclasticum and closely related strains in the rumen by competitive PCR. Appl Environ Microbiol. 64: 907-913.

SAS (2001) Institue Inc., SAS/STAT Software : Changes and Enhancements, Release 8.2, Cary, NC, USA.

Shinkai, T. and Kobayashi, Y. (2007). Localization of ruminal cellulolytic bacteria on plant fibrous materials as determined by fluorescence in situ hybridization and real-time PCR. Appl Environ Microbiol. 73:1646-1652.

Steel, R. G. D. and Torries, J. H. (1980). Principles and Procedures of Statistics: a Biometeric Approach (2nd Ed). McGrow Hill, New York.

Tajima, K., Nagamine, T., Matsui, H., Nakamura, M. and Aminov, R. I. (2001). Phylogenetic analysis of archaeal 16S rRNA libraries from the rumen suggests the existence of a novel group of archaea not associated with known methanogens. FEMS Microbiology Letters. 200:67-72.

Van Soest, P. J., Robertson, J. B. and Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science. 74:3583-3597.

Yu, Z., Morrison, M. (2004). Improved extraction of PCR-quality community DNA from digesta and fecal samples. BioTechniques. 36:808-812.

Zimmermann, K. and Mannhalter, J. W. (1996). Technical aspects of quantitative competitive PCR. BioTechniques. 21:268-279.