Screening of phosphate solubilizing bacteria from ex-coal mining soil in Central Bengkulu District, Indonesia
Article Sidebar
Main Article Content
Abstract
The results of bacterial isolation obtained 402 bacterial isolates showed a clear zone, wherein 48 bacterial isolate had the potential ability to dissolve phosphate. From the 48 bacterial isolates, 10 which performed the highest phosphate solubilizing index (PSI) were selected. The PSBs selected PSBs were 9 gram-positive and 1 gram-negative. Biochemical test results showed that 7 isolates were closed to the genus Bacillus, 2 isolates were closed to Mycobacterium, and 1 isolate was closed to Serratia. Out of the 10 isolates, 2 best isolates were proceeded for quantitative performance test for solubilizing phosphate. The quantitative test revealed that BB3B27 and BB3B6 dissolved the highest phosphate on the 7th day, with 217.2 mg/L and 192.4 mg/L respectively. The 16S rRNA marker molecular showed that the selected BPF isolate BB3B27 had a 100% similarity in the nucleotide base sequence with the Bacillus subtilis strain BaBc-1.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Agus, C., Putra, P. B., Faridah, E., Wulandari, D. and Napitupulu, R. N. P. (2016) Organic carbon stock and their dynamics in rehabilitation ecosystem areas of post open coal mining at tropical region. Procedia Eng, 159:329-337.
Archana, D. S. (2007). Studies on Potassium Solubilizing Bacteria. Dharwad (IN). University of Agricultural Sciences.
Biswas, J. K., Banerjee, A., Rai, M., Naidu, R., Biswas, B., Vithanage, M., Dash, M. C, Sarkar, S. K. and Meers E. (2018). Potential application of selected metal resistant phosphate solubilizing bacteria isolated from the gut of earthworm (Metaphire posthuma) in plant growth promotion. Geoderma, 330:117-124.
Dick, D. P, Knicker, H., Avila, L. G., Inda Jr, A. V., Giasson, E. and Bissani, C. A. (2006). Organic Matter in Constructed Soils from A Coal Mining Area in Southern Brazil. Org. Geochem, 37:1537-1545.
Fardiaz, S. (1989). Mikrobiologi Pangan. PAU pangan dan Gizi IPB. Bogor.
Fardiaz, S. (1992). Mikrobiologi Pangan 1. PT. Gramedia Pustaka Utama. Bogor.
Gatson, J. W., Benz, B. F., Chandrasekaran, C., Satomi, M., Venkateswaran, K. and Hart, M. E. (2006). Bacillus tequilensis sp. nov., isolated from a 2000-year-old Mexican shaft-tomb, is closely related to Bacillus subtilis. International Journal of Systematic and Evolutionary Microbiology, 56:1475-1484.
Hii, Y. S., San Chan, Y., Lau, S. W. and Michael, D. (2020). Isolation and Characterisation of Phosphate Solubilizing Microorganisms From Peat. Biocatalysis and Agricultural Biotechnology, 26:101463.
Irianto, K. (2006). Mikrobiologi, Menguak Dunia Mikroorganisme Jilid 1. CV. Yrama Widya. Bandung.
Joner, E. J. I., Aarle, I. M. and Vosatka, M. (2000). Phosphatase activity of extraradical arbuscular mycorrhiza hypae: a review. Plant Soil, 226:199-210.
Kishore, N., Pindi, P. K. and Reddy, S. R. (2015). Phosphate-solubilizing microorganisms: a critical review. In: Bahadur B, Venkat Rajam M, Sahijram L, Krishnamurthy K (eds) Plant Biology and Biotechnology. Springer, New Delhi, pp.307-333.
Lay, B. W. (1994). Analisis Mikroba di Laboratorium. PT. Raja Grafindo Persada. Jakarta.
Lynn, T. M., Win, H. S., Kyaw, E. P., Latt, Z. K. and Yu, S. S. (2013). Characterization of Phosphate Solubilizing and Potassium Decomposing Strains and Study on Their Effects on Tomato Cultivation. International Journal of Innovation and Applied Studies, 3:959-966.
Marchesi, J. R., Sato, T., Weightman, A. J, Martin, T. A, Fry, J. C., Hiom, S. J. and Wade, W. G. (1998). Design and Evaluation of Useful Bacterium-Specific PCR Primers that Amplify Genes Coding for Bacterial 16S rRNA. Applied and Environmental Microbiology, 64:795-799.
Mursyida, E., Mubarik, N. R. and Tjahjoleksono, A. (2015). Selection and Identification of Phosphate-Potassium Solubilizing Bacteria from the Area Around the Limestone Mining in Cirebon Quarry. Research Journal of Microbiology, 10:270-279.
Nacoon, S., Jogloy, S., Riddech, N., Mongkolthanaruk, W., Kuyper, T. W. and Boonlue, S. (2020). Interaction Between Phosphate Solubilizing Bacteria and Arbuscular Mycorrhizal Fungi on Growth Promotion and Tuber Inulin Content of Helianthus tuberosus L. Scientific Reports, 10:1-10.
Novianti, V., Artha, W. S, Akhsani, F., Arrosadi, B. M. and Berliana, M. (2021). Changes in Microbial Composition on Plowed Coal Mines Overburden. AIP Conference Proceedings, 2353:030008.
Pelczar, M. J., Chan, E. C. S. and Krieg. (1976). Microbiology. Me Graw Hill Book Company. New York.
Pelczar, M. J. and Chan, E.C. S. (1986). Dasar-Dasar Mikrobiologi, Elements of Microbiology , 1. Hadioetomo RS, Imas T, Tjitrosomo SS, Angka SL. UI Pr. Jakarta (ID).
Premono, M. E., Moawad, A.M. and Vlek, P. L. G. (1996). Effect of phosphate-solubilizing Pseudomonas putida on the growth of maize and its survival in the rhizosphere. Indonesian Journal of Crop Sciences, 11:13-23.
Rahayu, Y. S., Yuliani, M. T. and Asri. (2020). Isolation and Identification of Endophytic Bacteria Related to Plant Nutrient Level in Coal Mining Site From East Kalimantan Indonesia. Advances in Engineering Research, 209:485-491.
Rawat, P., Das, S., Shankhdhar, D. and Shankhdhar, S. C. (2020). Phosphate-Solubilizing Microorganisms: Mechanism and Their Role in Phosphate Solubilization and Uptake. Journal of Soil Science and Plant Nutrition, 1-20.
Ren, Y., Strobel, G., Sears, J. and Park, M. (2010). Geobacillus sp., a thermophilic soil bacterium producing volatile antibiotics. Microbial Ecology, 60:130-136.
Robberts, M. S, Nakamura, L. K. and Cohan, F. M. (1996). Bacillus vallismortis sp. nov., a close relative of Bacillus subtilis, isolated from soil in Death Valley, California. International journal of systematic bacteriology, 46:470-475.
Sambrook, J. and Russel, R. W. (2001). Moleculer Cloning: A Laboratory Manual, 3rd ed. Cold Spring Harbor Laboratory Press. New York.
Slepecky, R. A. and Henpill. H. R. (1992). The genus Bacillus-nonmedical. in: Balows A, Trupper HG, Dworkin M, Harder W, Schleifer KH. editor. The Prokaryotes. Ed 2th. Springer-Verlay. New York.
Sukarman and Gani, R. A. (2020). Ex-coal mine land and their land suitability for agricultural commodities in South Kalimantan. Journal of Degraded and Mining Lands Management, 7:2171-2183, DOI: 10.15243/jdmlm. 2020.073.2171
Teng, Z., Shao, W., Zhang, K., Huo, Y. and Li, M. (2019). Characterization of Phosphate Solubilizing Bacteria Isolated From Heavy Metal Contaminated Soils and Their Potential for Lead Immobilization. The Journal of Environmental Management, 231:89-197.
Veith, B., Herzberg, C., Steckel, S., Feesche, J., Maurer, K. H. and Ehrenreich, P. (2004). The complete genome sequence of Bacillus licheniformis DSM13, an organism with great industrial potential. Journal of Microbiology and Biotechnology, 7:204-211.
Wei, Y., Zhao, Y., Fan, Y., Lu, Q., Li, M., Wei, Q., Zhao, Y., Cao, Z. and Wei, Z. (2017). Impact of phosphate-solubilizing bacteria inoculation methods on phosphorus transformation and long-term utilization in composting. Bioresource Technology, 241:134-141.
Whitmore, A. P, Whalley, W. R., Bird, N. R. A., Watts, C.W. and Gregory, A. S. (2011). Estimating Soil Strength in the Rooting Zone of Wheat. Plant and Soil, 339:363-375.
