Callus Formation of Curcuma mangga in MS Media Supplemented by 2.4-Dichlorophenoxyacetic Acid
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Abstract
Callus induction in Curcuma mangga was investigated in three different explant sources. At the concentrations of 0.5 to 2.5 ppm, it was shown that 2.4-D significantly affected on callus induction of C. mangga. Supplementation of 0.5 to 1 ppm of 2.4-D on MS media produced larger of callus diameter than higher concentration of 2.4-D. Increasing 2.4-D concentration tended to reduce the size and weight of callus. There was no callus formation in the MS media without 2.4-D. Apical bud was the better source of explant compared to lateral bud or leaf lamina indicated by the earlier callus initiation, larger callus diameter and higher callus fresh weight. The calli formed from apical bud were friable and white in color. Apical bud culture in MS media without 2.4-D produced a considerably high number of shoots. All source of explants showed similar response to the supplementation of 2.4-D on MS media.
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