In vitro propagation of Musa acuminata (AAA group) cv. ‘Kluai Nak’

Abstract

In vitro propagation of Musa acuminata (AAA group) cv. ‘Kluai Nak’ was studied on 4 factors such as sterilization method, shoot induction, root induction and acclimatization. Shoot explants were sterilization with 30% sodium hypochlorite for 30 minutes gave the highest survival rate (97.50%). For the shoot induction and multiplication, the result found that adding 4 mg/L BA on culture medium gave the highest number of shoots (3.20 shoots/explant) and number of leaves (9.20 leaves/explant), while culture medium without BA gave the highest height (5.90 cm). After the shoot multiplication, the root induction carried out by adding 2.5 mg/L NAA without AC gave the highest number of roots (25.80 roots/plant) but it was shot root, not complete plantlet and healthy plant for growth so the next experiment was chosen the plant from culture medium supplemented with 1.5 mg/L NAA and 0.2% activated charcoal that gave the number of roots (17.00 roots/plant) and root length (4.07 cm). After the root induction, the plantlet was successfully acclimatization in the combination of rice husk mixed potting soil that found to produce the highest percentage of plantlet survival rate with 90% and the plant height with 8.40 cm that obtained after 4 weeks of acclimatization.