Isolation and characterization of plant-growth promoting fungi associated with indigenous fermented amendment from root vicinity of Phyllostachys aurea
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Abstract
Results revealed that ITS sequence analyses expressing five isolated fungi belonging to Ascomycota, with three distinct genera, Aspergillus, Cladosporium and Parengyodontium were phylogenetically determined which closed to the reference strains. Most of the isolates were categorized as dark septate endophytes in appearances with exception of white mycelium Parengyodontium album IMX 2. The result showed that Parengyodontium album IMX 2 had a maximum IAA productivity of 21.10 ± 1.04 μg/mL in the presence of 500 mg/L tryptophan. The fungal isolates of Cladosporium sphaerospermum IMX 1, Parengyodontium album IMX 2, Cladosporium endophytica IMX 3 and Aspergillus pseudotamarii IMX 8 exhibited variable efficiency for solubilizing tricalcium phosphate salts. Moreover, Cladosporium sphaerospermum IMX 1 significantly suppressed the growth of Fusarium oxysporum and Botrytis cinerea by 49.64% and 76.73% respectively as compared to other isolates. These results suggested that IMOs and their plant growth-promoting fungi potentially could be used as a beneficial resource in promoting sustainable agriculture, which may act as a possible substitute for chemical fertilizers.
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References
Abu-Bakar, N. and Ibrahim, N. (2013). Indigenous microorganisms production and the effect on composting process. AIP Conference Proceedings, 1571:283-286.
Anyanwu, C. F., Ngohayon, S. L., Ildefonso, R. L. and Ngohayon, J. L. (2013). Isolation and characterization of indigenous microorganism (IMO) from Ifugao bamboo (Phyllostachys aurea) forest. International Journal of Science and Research, 4:1319-1324.
Bánfi, R., Pohner Z., Szabó, A., Herczeg, G., Kovács, G. M., Nagy, A., Márialigeti, K. and Vajna, B. (2021). Succession and potential role of bacterial communities during Pleurotus ostreatus production. FEMS Microbiology Ecology, 97: fiab125.
Belfiori, B., Rubini, A. and Riccioni, C. (2019). Diversity of endophytic and pathogenic fungi of saffron (Crocus sativus) plants from cultivation sites on Italy. Diversity, 13:535.
Berthelot, C., Perrin, Y., Leyval, C. and Blaudez, D. (2017). Melanziation and agening are not drawbacks for succesful agro-transformation of dark septate endophytes. Fungal Biology, 121:652-663.
Day, M. J. and Currah, R. S. (2011). Role of selected dark septate endophyte species and other hyphomycetes as saprobes on moss gametophytes. Botany, 89:349-359.
Farias, C. P., de Carvalho, R. C., Resende, F. M. L. and Azevedo, L. C. B. (2018). Consortium of five fungal isolates conditioining root growth and arbuscular mycorrhiza in soybean, corn, and sugarcane. Anais da Academia Brasiliera de Ciėncias, 90:3649-3660.
Fonseca, S., Radhakrishnan, D., Prasad, K. and Chini, A. (2018). Fungal production and manipulation of hormones. Current Medicinal Chemistry, 25:253-267.
Fu, S-F., Sun, P-F., Lu, H-Y., Wei, J-Y., Xiao, H-S., Fang, W-T., Cheng, B-Y. and Chou, J-Y. (2016). Plant growth-promoting traits of yeast isolated from the phyllosphere and rhizosphere of Drosera spatulata Lab. Fungal Biology, 120:433-448.
Ganesh, S., Khan, F., Ahmed, M. K., Velavendan, P., Pandey, N. K. and Kamachi Mudali, U. (2012). Spectrophotometric determination of trace amounts of phosphate in water and soil. Water Science & Technology, 66:2653-2658.
Hamayun, M., Khan, S. A., Ahmad, N., Tang, D-S., Kang, S-M., Na, C-I., Sohn, E-Y., Hwang, Y-H., Shin, D-H., Lee, B-Y., Kim, J-G. and Lee, I-J. (2009). Cladosporium sphaerospermum as a new plant-growth-promoting endophyte from the roots of Glycine max L. Merr. World Jornal of Microbiology and Biotechnology, 25:627-632.
Hossain, M. M., Sultana, F. and Hyakumachi, M. (2017). Role of ethylene signalling in growth and systemic resistance induction by the plant growth promoting fungus Penicillium viridicatum in Arabidopsis. Journal of Phytopathology, 165:432-441.
Jain, R., Saxena, J. and Sharma, V. (2017). The ability of two fungi to dissolve hardly soluble phosphates in solution. Mycology, 8:104-110.
Jan, U., Feiwan, R., Masood, J. and Chun, S. C. (2020). Characterization of soil microorganism from humus and indigenous microorganism amendments. Mycobiology, 5:392-398.
Khalil, A. M. A., Hassan, S. E-D., Alsharif, S. M., Eid, A. M., Ewais, E. E-D., Azab, E., Gobouri, A. A., Elkelish, A. and Fouda, A. (2021). Isolation and characterization of fungal endophytes isolated from medicinal plant Ephedra pachyclada as plant growth-promoting. Biomolecules, 11:140.
Kriaa, M., Hammami, I., Sahnoun, M., Azebou, M.C., Triki, M. A. and Kammoun, R. (2015). Purification, biochemical characterization and antifungal activity of a novel Aspergillus tubingensis glucose oxidase steady on broad range of pH and temperature. Bioprocess and Biosystems Engineering, 38:2155-2166.
Kumar, S., Stecher, G., Li, M., Knyaz, C. and Tamura, K. (2018). MEGA X: Molecular evolutionary genetics analysis across computing platforms. Molecular Biology and Evolution, 35:1547-1549.
Kumar, B. L. and Gopal, D. V. R. S. (2015). Effective role of indigenous microorganisms for sustainable environment. 3 Biotech, 5:867-876.
Likar, M. and Regvar, M. (2013). Isolates of dark septate endophytes reduce metal uptake and improve physiology of Salix caprea L. Plant and Soil, 370:593-604.
Malhotra, H., Vandana, Sharma, S. and Pandey, R. (2018). Phosphorus nutrition: plant growth in response to deficiency and excess. In M. Hasanuzzaman, M. Fujita, H. Oku, K. Nahar & B. Hawrylak-Nowak, eds. Plant Nutrition Abiotic Stress Tolerance, pp. 171-190. Spinger, Singapore.
Mohd Din, A. R. J., Azam, M. Z., Othman, N. Z. and Sarmidi, M. R. (2019). Paenibacillus polymyxa role involved in phosphate solubilization and growth promotion of Zea mays under abiotic stress condition. Proceedings of National of Academy of Sciences, India B Biological Sciences, 90:63-70.
Muslim, A., Hyakumachi, M., Kageyama, K. and Suwandi, S. (2019). Induction of systemic resistance in cucumber by hypovirulent binucleate rhizoctonia against anthracnose caused by Colletotrichum orbiculare. Tropical Life Sciences Research, 30:109-122.
Naher, L., Fatin, S. N., Sheikh, M. A. H., Azeez, L. A., Siddiquee, S., Zain, N. M. and Karim, S. M. R. (2021). Cellulase enzyme production from filamentous fungi Trichoderma reesei and Aspergillus awamori in submerged fermentation with rice straw. Journal of Fungi, 7:868.
Naziya, B., Murali, M. and Amruthesh, K. N. (2020). Plant-growth-promoting fungi (PGPF) instigate plant growth and induce disease resistance in Capsicum annuum L. upon infection with Colletotrichum capsici (Syd.) Butler & Bisby. Biomolecules, 10:41.
Nicolopoulou-Stamati, P., Maipas, S., Kotampasi, C., Stamatis, P. and Hens, L. (2016). Chemical pesticides and human health: the urgent need for a new concept in agriculture. Frontier in Public Health, 4:148.
Nyein, W. W., Kim, D. H. and Chun. S. C. (2017). Soil microbial population in relation to farming system. Korean Society of Mycobiology Spring Conference, Korea, 12p.
Phua, C. K. H., Abdul Wahid, A. N. and Abdul Rahim, K. (2012). Development of multifunctional biofertilizer formulation from indigenous microorganims and evaluation of their N2-fixing capabilities on Chinese cabbage using 15N tracer technique. Pertanika Journal of Tropical Agricultural Science, 35:673-679.
Rashid, M. I., Mujawar, L. H., Shahzad, T., Almeelbi, T., Ismail, I. M. I. and Oves, M. (2016). Bacteia and fungi can contribute to nutrients bioavailability and aggregate formation in degraded soils. Microbiological Research, 183:26-41.
Rustamova, N., Gao, Y., Zhang, Y. and Yili, A. (2020). Biological activity of endophytic fungi from the roots of the medicinal plant Vernonia anthelmintica. Microorganisms, 8:586.
Santoyo, G., Hernandez-Pacheco, C., Hernandez-Salmeron, J. and Hernandez-Leon, R. (2017). The role of abiotic factors modulating the plant-microbe-soil interactions: towards sustainable agriculture. A review. Spanish Journal of Agricultural Research, 15:1-15.
Sasirekha, B., Shivakumar, S. and Sullia, S. B. (2012). Statistical optimization for improved indole-3-acetic acid (IAA) production by Pseudomonas aeruginosa and demonstration of enhanced plant growth promotion. Journal of Soil Science and Plant Nutrition, 12:863-873.
Sharma, S. B., Sayyed, R. Z., Trivedi, M. H. and Gobi, T. A. (2013). Phospahte solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. SpringerPlus, 2:587.
Shen, Q., Liu, Y. and Naqvi, N. I. (2018). Fungal effectors at the crossroads of phytohormone signalling. Current Opinion in Microbiology, 46:1-6.
Skidmore, A. M. and Dickinson, C. H. (1976). Colony interactions and phyphal interference between Septoria nodorum and phylloplane fungi. Transactions of the British Mycology Society, 66:57-64.
Song, F., Tian, X., Fan, X. and He, X. (2010). Decomposing ability of filamentous fungi on litter is involved in a subtropical mixed forest. Mycologia, 102:20-26.
Tamayo-Velez, A. and Osorio, N. W. (2017). Co-inoculation with an arbuscular mycorrhizal fungus and a phosphate-solubilizing fungus promotes the plany growth and phosphate updatke of avocado plantlets in a nursery. Botany, 95:539-545.
Trump, C. (2016). Korean natural farming on large scale science and economics. Paper presented at the 1st International Symposium of Natural Science Farming Seoul, South Korea.
Tsang, C-C., Chan, J. F. W., Pong, W-M., Chen, J. H. K., Ngan. A. H. Y., Cheung, M., Lai, C. K. C., Tsang, D. N. C., Lau, S. K. P. and Woo, P. C. Y. (2016). Cutaneous hyalohyphomycosis due to Parengyodontium album gen. et comb. nov. Medical Mycology, 54:699-713.
Venturi, V. and da Silva, D. P. (2012). Incoming pathogens team up with harmless ‘resident’ bacteria. Trends in Microbiology, 20:160-164.
Whitelaw, M. A., Harden, T. J. and Helyar, K. R. (1999). Phosphate solubilization in solution culture by the soil fungus Penicillium radicum. Soil Biology and Biochemistry, 31:655-665.
Wang, X., Wang, C., Sui, J., Liu, Z., Li, Q., Ji, C., Song, X., Hu, Y., Wang, C., Sa, R., Zhang, J., Du, J. and Liu, X. (2018). Isolation and characterization of phosphofungi, and screening for their plant growth-promoting activities. AMB Express, 8:63.
Wu, H., Yang, H. Y., You, X. L. and Li, Y. H. (2013). Diversity of endophytic fungi from roots of Panax ginseng and their saponin yield capacities. SpringerPlus, 2:1-9.
Xa, L. T. and Nghia, N. K. (2020). Microbial diversity of indigenous microorganism communities from different agri-ecosystems in Soc Trang province Vietnam. Ho Chi Minh City Open University Journal of Science, 10:53-64.
Zhang, Y., Chen, F. S., Wu, X. Q., Luan, F. G., Zhang, L. P., Fang, X. M., Wan, S. Z., Hu, X. F. and Ye, J. R. (2018). Isolation and characterization of two phosphate solubilizing fungi from rhizosphere soil of moso bamboo and their functional capacities when exposed to different phosphorus sources and pH environments. PloS One, 13:7.
Zhang, H-C., Ma, Y-M., Liu, R. and Zhou, F. (2012). Endophytic fungus Aspergillus tamarii from Ficus carica L., a new source of indoyl diketopiperazines. Biochemical Systematics and Ecology, 45:31-33.
