Evaluation of SNP markers linked to the THCA and CBDA synthase gene for varietal improvement of Cannabis sativa L.
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Abstract
This study compared the nucleotide variation of the THCAS gene between the high THC (Type I) and high CBD (Type III) of cannabis plants. The results showed that 12 of 63 SNP positions were selected as having the potential to distinguish between these two types. Validation of the 12 SNPs was performed in F1 progeny derived from the cross between Type III (DNM) and Type I (CHP) plants using a pyrosequencing technique and in silico analysis. The results revealed that the SNP positions 869 (T/C) and 881 (T/G) could be used as effective markers to predict the cannabinoid type of cannabis plant correctly but not the quantity. Finally, the tetra-primer ARMS-PCR was designed to be used as the Marker-Aided Selection in the cannabis breeding program
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