A study on the anti-Helicobacter pylori activity of three medicinal plant crude extracts from Cannabis sativa, Mitragyna speciosa and Phyllanthus emblica

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Published: May 5, 2026
Keywords:
Medicinal plant crude extracts Helicobacter pylori Synergistic effect Scanning electron microscopy

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Khruethuen, S.
College of Agricultural Innovation and Food Technology, Rangsit University, Pathum Thani 12000, Thailand
Pilasombut, P.
Office of Administrative Interdisciplinary Program on Agricultural Technology, School of Agricultural Technology, King Mongkut’s Institute of Technology Ladkrabang, Bangkok, 10520, Thailand
Woraprayote, W.
Department of Biochemistry, Siriraj Metabolomics and Phenomics Center (SiMPC), Faculty of Medicine Siriraj Hospital, Mahidol University, Bangkok 10700, Thailand
Janyaphisan, T.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand
Pumpuang, L.
National Center for Genetic Engineering and Biotechnology (BIOTEC), National Science and Technology Development Agency (NSTDA), Pathum Thani, 12120, Thailand
Maniin, P.
Faculty of Agricultural Innovation, College of Agricultural Innovation and Food, Rangsit University, Lak Hok, Thailand
Yahuafai, J.
Division of Research and Technology Assessment, National Cancer Institute, Bangkok 10400, Thailand.
Rumjuankiat, K.
College of Agricultural Innovation and Food Technology, Rangsit University, Pathum Thani 12000, Thailand

Abstract

In the present study, medicinal plant crude extracts were prepared from cannabis (Cannabis sativa), kratom (Mitragyna speciosa) and Indian gooseberry (Phyllanthus emblica) using four different solvents including ethanol, methanol, isopropanol, and water. The methanolic crude extracts had the highest weight and percentage yield, whereas the aqueous crude extracts exhibited the lowest values. The antimicrobial activity of these extracts against three Helicobacter pylori strains (ATCC 43504, 2888, and BK364) was evaluated using the spot-on-lawn method. The obtained results indicated that all medicinal plant crude extracts had an inhibitory effect against all tested H. pylori strains. The methanolic and ethanolic extracts of Cannabis sativa inhibited H. pylori strains ATCC 43504, 2888, and BK364 at the minimum inhibitory concentration (MIC) values ranging from 0.02-0.05 mg/mL. Additionally, a synergistic effect between Cannabis sativa, Mitragyna speciosa, and Phyllanthus emblica was investigated using the checkerboard assay. The synergistic interaction was observed between the Cannabis sativa, Mitragyna speciosa, and Phyllanthus emblica extracts, with a fractional inhibitory concentration index (FICI) of 2, indicating an indifferent effect when combined. Furthermore, the scanning electron microscopy (SEM) investigation revealed that the H. pylori cells were damaged after treatment with methanolic crude extracts of cannabis for 120 minutes.

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How to Cite
Khruethuen, S., Pilasombut, P., Woraprayote, W., Janyaphisan, T., Pumpuang, L., Maniin, P., Yahuafai, J., & Rumjuankiat, K. (2026). A study on the anti-Helicobacter pylori activity of three medicinal plant crude extracts from Cannabis sativa, Mitragyna speciosa and Phyllanthus emblica. International Journal of Agricultural Technology, 22(3), 1167–1178. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/11681
Issue
Vol. 22 No. 3 (2026): May
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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