Streptomyces corchorusii L72 as a potential biocontrol agent against soil born fungi Sclerotium rolfsii causing stem rot on peanut
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Abstract
Streptomyces is a distinctive genus that contains a variety of naturally produced antibiotics and active secondary substances. From 37 actinomyces isolates, isolate L72 was selected because of its highest ability to inhibit Sclerotium rolfsii (63.59% inhibition). The antagonistic activity and growth-promoting properties of strain L72 were investigated. This isolate was identified as Streptomyces corhorsii L72 based on morphological and physiological properties and analysis of the 16S rRNA gene sequence. Culture filtrate of strain L72 exhibited antagonism activities on mycelial growth and scletorial germination rates of S. rolfsii at various diluted concentrations. Interestingly, scletorial germination of S. rolfsii was inhibited on the medium with only 2% (50X dilution) of L72’s culture filtrate. Biochemical assays revealed that strain L72 produced indole acetic acid, siderophore, and chitinase. The strain also exhibited the ability to solubilize phosphate. Moreover, cell-free culture of this strain promoted peanut fresh weight, root length, and seedling vigor. The data from the pot assay showed that the treatment with Streptomyces corhorsii L72 reduced disease incidences when compared with the inoculated control. In conclusion, our results indicated that Streptomyces corhorsii L72 was a promising biocontrol agent for controlling stem rot disease.
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