Evaluating the mycelial growth of bolete from pine forest in highland Vietnam
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Abstract
Ectomycorrhizal fungi were difficultly isolates in artificial culture. The evaluation of mycelial preservation was important for predicting the macrofungal cultivated potential of this group. In this study, 24 bolete sporocarps were collected from Pinus kesiya pine forests at Da Nhim Watershed Protection Forest which were successfully isolated. Basing on macroscopic characteristics and molecular phylogenetic retrieved from ITS markers, all sporocarps were identified to be 5 genera as Suillus, Boletus, Baorangia, Leccinum and Tylopilus. All fungal isolates were evaluated the mycelial growth and then preserved on MMN agar medium at
10 oC. Suillus with the best mycelium growth potential was significantly different from the others. After 1 year and 2 years of storage, 24 fungal isolates were evaluated the mycelial growth by both MMN broth and agar. The results showed that all isolates which belongs to Suillus were viable after 2 years preservation except isolate A04101. These are preserved the ectomycorrhizal fungi resources for studies about inoculation, biochemistry and artificial culture.
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