Influence of plant growth regulators on shoot development of Chrysanthemums
Main Article Content
Abstract
Plant nodes were cultured on MS mediums supplemented with multiple concentrations of plant growth regulators. Sunny Snow produced the largest callus of 1.19×1.19 cm and obtained 93.33% of new shoots with 10.00 shoots per callus, and 0.84 g callus fresh weight, when grew in MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA as compared to the control which no callus was produced. After 20 weeks of culturing, the second set showed that the control treatment of Sunny Snow gave the highest stem growth and the best canopy size of 9.00 and 5.60 cm respectively. Chompoo Phan gave the highest scores of chlorophyll content, i.e., chl a 652.14 µg/g FW, chl b 332.47 µg/g FW and carotenoid 165.92 µg/g FW. Besides, MDA appeared to be highest, 17.37 nmol/g FW when cultured on MS medium supplemented with 1 mg/l BA + 0.1 mg/l IAA.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Akaneme, F. I. and Eneobong, E. E. (2008). Tissue culture in Pinus caribaea Mor. var. Hondurensis barr. and golf. II: effects of two auxins and two cytokinins on callus growth habits and subsequent organogenesis. African Journal of Biotechnology, 7:757-765.
Alsoufi, A. S. M. M., Ahmed, Z. S. and Salim, A. M. (2021). The efficiency of interaction between cytokines and auxins in micropropagation of chrysanthemum plant (Chrysanthemum Indicum L.). IOP Conference Series: Earth and Environmental Science, 735:12048.
Bhattacharya, P., Dey, S., Das, N. and Bhattacharya, B. C. (1990). Rapid mass propagation of Chrysanthemum morifolium by callus derived from stem and leaf explants. Plant Cell Rep, 9:439- 442.
Brock, T. C. and Kaufman, P. B. (1991). Growth regulators: an account of hormone and growth regulation. London: Growth and Development. Academic Press Incorporated, pp.277-340. In F.C. steward (ed.). Plant Physiology: A Treatise. Vol 10.
Chen, Y. Z., He, X. D., Jiang, P. Y. and Wang, C. M. (1985). In vitro propagation of chrysanthemum leaves. Journal Jinagsu Agriculture, 6:33-36.
Gahan, P. B. and George E. F. (2008). Adventitious regeneration, In George, E. F., M. A. Halls and G. D. Klerk eds. plant propagation by tissue culture: Vol 1. The Springer, Netherlands.
George, E. G. and Sherrington, P. D. (1984). Plant propagation by tissue culture. Handbook and directory of commercial laboperations. Exegetics Limited, Basingstoke, pp.709.
Heath, R. L. and Packer, L., (1968). Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125:189-198.
Jafari, A., Kahrizi, D. and Mansouri, M. (2016). Effects of plant growth regulators and explant on callus induction in pennyroyal (Mentha pulegium L.). Biharean Biologist, 10:134- 136.
Kaweeta, R. (1998). Plant tissue culture: formality and techniques. Vol 2. Kasetsart University, Bangkok.
Krasaechai, A. (1992). Chrysanthemum. Odeon Store Publisher. Bangkok.
Kumar, S., Prasad, K. V. and Choudhary, M. L. (2006). Detection of genetic variability among chrysanthemum cadio-mutants using RAPD markers. Current Science, 90:1108-1113.
Lichtenthaler, H. K. and Buschmann, C. (2001). Chlorophylls and carotenoids: ceasurement and characterization by UV-VIS spectroscopy. Current Protocols in Food Analytical Chemistry, F4.3.1-F4.3.8.
Limsanguan, P., Chanprame, S. and Chanprame, S. (2017). Callus induction and plantlets regeneration from mature Ray Floret of Chrysanthemum. Agricultural Sciences Journal, 48:322-333.
Marnett, L. J. (1999). Lipid peroxidation-DNA damage by malondialdehyde. Mutation Research, 424:83-95.
Momoko, I., Keiko, S. and Akira, I. (2013). Plant callus: mechanisms of induction and repression. The Plant Cell, 25:3159-3173.
Murashige, T. and Skoog, F. (1962). A revised medium for rapid grow and bioassay with tobacco tissue culture. Plant Physiology, 15:473-497.
Nahid, J. S., Shyamali, S. and Kazumi, H. (2007). High frequency shoot regeneration from petal explants of Chrysanthemum morifolium Ramat. in vitro. Pakistan Journal of Biological Sciences, 10:3356‐3361.
Nasri, F., Zakizadeh, H., Vafaee, Y. and Mozafari, A. A. (2018). Callus induction and plant regeneration of Chrysanthemum morifolium and C. coccineum via direct and indirect organogenesis and genetic fidelity analysis using IRAP, ISSR and SCoT molecular markers. The Journal of Ornamental Plants, 8:265-284.
Satish, L., Ceasar, S. A., Shilpha, J., Rency, A. S., Rathinapriya, P. and Ramesh, M. (2015). Direct plant regeneration from In vitro derived shoot apical meristems of finger millet (Eleusine coracana L. Gaertn). In vitro Cellular and Develomental Biology-Plant, 51:192-200.
Scott, P. (2008). Physiology and behaviour of plants. John Wiley and Sons Incorporated, Hoboken, USA, pp.20-21.
Scrivanti, L. R., Zunino, M. P. and Zygadlo, J. A. (2003). Tagetes minuta and Schinus areira essential oils as allelopathic agents. Biochemical Systematics and Ecology, 31:563–572.
Shahidul, Md. I. K., Fahima, K., Sadia, A. and Hoque, M. E. (2020). Callus induction and plantlet regeneration in chrysanthemum. International Journal of Business, Social and Scientific Research, 8:6-10.
Sun, M., Davey, M. R. and Power, J. B. (2007). In vitro propagation of chrysanthemum CV. Cheng Yun through callus culture. Plant Tissue Culture, 11:56-62.
Teixeira da Silva, J. A. (2014). Organogenesis from chrysanthemum (Dendranthema x grandiflora (Ramat.)) ‘Kitamura’ petals (disc and ray florets) induced by plant growth regulators. Asia-Pacific Journal of Molecular Biology and Biotechnology, 22:145-151.
Teixeira da Silva, J. A., Shinoyama, H., Aida, R., Matsushita, Y., Raj, S. K. and Chen, F. (2013). Chrysanthemum biotechnology: Quo vadis. Critical Reviews in Plant Sciences, 32:21- 52.
Thangmanee, C. and Kanchanapoom, K. (2011). regeneration of chrysanthemum plants (Chrysanthemum x grandiflorum (Ramat.) Kitam.) by callus derived from ray floret explants. Propagation of Ornamental Plants, 11:204-209.
Tymoszuk, A. and Zalewska, M. (2014). In vitro adventitious shoots regeneration from ligulate florets in the aspect of application in chrysanthemum breeding. Acta Scientiarum Polonorum Hortorum Cultus, 13:69-82.
Xu, Z., Um, Y. C., Kim, C. H., Lu, G., Guo, D. P., Liu, H. L., Bah, A. A. and Mao, A. (2008). Effect of plant growth regulators, temperature and sucrose on shoot proliferation from the stem disc of Chinese jiaotou (Allium chinense) and in vitro bulblet formation. Acta Physiologiae Plantarum, 40:521-528.