In vitro skin protection effects of sulfated exopolysaccharide fragment derived from the cultivation of Ophiocordyceps sinensis
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Abstract
Exopolysaccharides (EPS) are extracted from a cultivated medium of Ophiocordyceps sinensis. EPS extraction was deproteinized and filtrated to remove EPS fragments weighing higher than 100kDa. The sulfur trioxide-pyridine (SO3.Py) method was conducted to enhance the biological activity of exopolysaccharides. The degree of sulfate substitution (DS) of SEPS11 is the highest 1.79 corresponding with 34.90% of sulfate amount at 80 oC. Increasing the EPS fragment ratio decreased the DS to 1.20 and 24.67% of the sulfate amount. Sulfated exopolysaccharides exhibited higher levels of antioxidant and tyrosinase inhibitor activities, which showed a correlation with sun protection factors. The tyrosinase inhibitor, antioxidant and activities of SEPS11 are the highest, and SPF of 15.66 mg/mL and 1.05 respectively. Sulfated O. sinensis EPS fragments weighted less than 100 kDa have the potential in the application of cosmetics or functional food as a skin protection additive.
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