Influence of exogenous salicylic acid on phytochemical improvement and antioxidant activity in Cannabis sativa L.

Main Article Content

Tebdoie, C.
Dewatthanawong, R.
Kongjinda, P.
Montri, N.

Abstract

The impact of salicylic acid (SA) foliar spray at varied concentrations (0 and 1 M) and preharvest periods (24, 36, and 42 hours) on secondary metabolite in inflorescences of cannabis (Cannabis sativa L.) was investigated. Result was significantly differed in secondary compound composition. Application of 1 M SA at 24 hours preharvest increased total phenolics, total flavonoids, and total chlorophyll. Moreover, 1 M SA at 24 and 42 hours preharvest showed higher chlorophyll a and b contents than 1 M SA at 36 hours preharvest. While antioxidant activity was not significantly differed among 1 M SA treatments at different preharvest periods, it surpassed non-SA-treated plants. The total pigment was not significantly differed among SA treatments. 1 M SA spray reduced carotenoid content in cannabis inflorescences, with the highest carotenoid observed in non-SA treated plants.

Article Details

How to Cite
Tebdoie, C., Dewatthanawong, R., Kongjinda, P., & Montri, N. (2024). Influence of exogenous salicylic acid on phytochemical improvement and antioxidant activity in Cannabis sativa L. International Journal of Agricultural Technology, 20(1), 365–380. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/12259
Section
Original Study

References

Abdi, G. and Karami, L. (2020). Salicylic acid effects on some physiochemical properties and secondary metabolite accumulation in Mentha piperita L. under water deficit stress. Advances in Horticultural Science 34:81-91.

Abrams, D. I. (2022). Cannabis, cannabinoids and cannabis-based medicines in cancer care. Integrative Cancer Therapies 21:1-11.

Ahmed, Z. F., Kaur, N., Maqsood, S. and Hirschmann, G. S. (2022). Preharvest applications of chitosan, salicylic acid, and calcium chloride have a synergistic effect on the quality and storability of date palm fruit (Phoenix dactylifera L.). HortScience 57:422-430.

Ali, B. (2021). Salicylic acid: An efficient elicitor of secondary metabolite production in plants. Biocatalysis and Agricultural Biotechnology 31:101884.

Arif, Y., Sami, F., Siddiqui, H., Bajguz, A. and Hayat, S. (2020). Salicylic acid in relation to other phytohormones in plant: A study towards physiology and signal transduction under challenging environment. Environmental and Experimental Botany 175:104040.

Basker, R., Shrisakthi, S., Sathyapriya, B. B., Sathyapriya, R., Nithya, R. and Poongodi, P. (2010). Antioxidants potential of peel extracts of banana varieties (Musa sapientum). Food and Nutrition Sciences 2:1128 -1133.

Berman P., Futoran K., Lewitus G. M., Mukha, D., Benami, M. and Shlomi, T. (2018). A new ESI-LC/MS approach for comprehensive metabolic profiling of phytocannabinoids in Cannabis. Scientific Reports 8:1-15.

Blanch, G. and Maria, J. G. (2020). Exogenous salicylic acid improves phenolic content and antioxidant activity in table grapes. Plant Foods for Human Nutrition 75:177-183.

Gacnik, S., Veberič, R., Hudina, M., Marinovic, S., Halbwirth, H. and Petkovšek, M. M. (2021). Salicylic and methyl salicylic acid affect quality and phenolic profile of apple fruits three weeks before the harvest. Plants 10:1-15.

Gao, Z., Meng, C., Zhang, X., Xu, D., Miao, X., Wang, Y., Yang, L., Lv, H., Chen, L. and Ye, N. (2012). Induction of salicylic acid (SA) on transcriptional expression of eight carotenoid genes and astaxanthin accumulation in Haematococcus pluvialis. Enzyme and Microbial Technology 51:225-30.

Ghasemzadeh, A. and Jaafar, H. Z. (2013). Interactive Effect of Salicylic Acid on Some Physiological Features and Antioxidant Enzymes Activity in Ginger (Zingiber officinale Roscoe). Molecules 18:5965-5979.

Halder, M., Sarkar, S. and Jha, S. (2019). Elicitation: A biotechnological tool for enhanced production of secondary metabolites in hairy root cultures. Engineering in Life Sciences 19:880-895.

Ibrahim, M. H., Omar, H. and Amalina, N. (2017). Salicylic acid enhanced photosynthesis, secondary metabolites, antioxidant and lipoxygenase inhibitory activity (LOX) in Centella asiatica. Annual Research & Review in Biology 17:1-14.

Jiang, H. E., Zhao, L. X., Ferguson, Y. X., Hueber, D. K., Bera, F., Wang, S., Zhao, Y. F., Liu C. J. and Li, C. S. (2006). A new insight into Cannabis sativa (Cannabaceae) utilization from 2500-year-old Yanghai Tombs, Xinjiang, China. The Journal of Ethnopharmacology 108:414-422.

Jin, D., Dai, K., Xie, Z. and Chen, J. (2020). Secondary metabolites profiled in Cannabis inflorescences, leaves, stem barks, and roots for medicinal purposes. Scientific Reports 10:3309.

Jirakiattikul, Y., Rithichai, P., Songsoe, K. and Itharat, A. (2021). Elicitation of salicylic acidon secondary metabolite production and antioxidant activity of In Vitro Musa acuminata L. cv. ‘Gros Michel’ Shoots. Current Applied Science and Technology 21:569-578.

Kalant, H. (2001). Medicinal Use of Cannabis: History and Current Status. Pain research & management: the journal of the Canadian Pain Society. journal de la société canadienne pour le traitement de la douleur 6:80-91.

Kandar, C. C. (2020). Secondary metabolites from plant sources. Bioactive Natural Products for Pharmaceutical Applications, pp.329-377.

Khattak, A. F., Nakhli, K. M. Arfaj, A. K. M. and Cheema, A. A. (2018). Comparison of outcomes and complications of deep anterior lamellar keratoplasty and penetrating keratoplasty performed in a large group of patients with keratoconus. Int Ophthalmol 38:985-992.

López M. I., Baltazar A. N. Y., Buchala, A. and Serrano, M. (2019). Intra and extracellular journey of the phytohormone salicylic acid. Frontiers in Plant Science 10:423.

Mahady, G. B., Liu, C. and Beecher, W. W. (1998). Involvement of protein kinase and G proteins in the signal transduction of 1582 benzophenanthridine alkaloid biosynthesis. Phytochemistry 48:93-102.

Mirzamohammad, E., Alirezalu, A., Alirezalu, K., Norozi, A. and Ansari, A. (2021). Improvement of the antioxidant activity, phytochemicals, and cannabinoid compounds of Cannabis sativa by salicylic acid elicitor. Food Science & Nutrition 9:6873-6881.

Najafian, S., Khoshkhui, M. and Tavallali, V. (2009). Effect of salicylic acid and salinity in Rosemary (Rosmarinus officinalis L.): Investigation on changes in gas exchange, water relations, and membrane stabilization. Advances in Environmental Biology 3:322-328.

Ramakrishna, A. and Ravishankar, G. A. (2011). Influence of abiotic stress signals on secondary metabolites in plants. Plant Signaling & Behavior 6:1720-31.

Rani, A., Guleria, M., Sharma, Y., Sharma, S., Chaudhary, A., Sharma, R. and KumarInsights, P. (2023). Insights into elicitor’s role in augmenting secondary metabolites production and climate resilience in genus Ocimum – A globally important medicinal and aromatic crop. Industrial Crops and Products 202:117078.

Raskin, I. (1992). Salicylate, a new plant hormone. Plant Physiology 99:799-803.

Rock, E. M. and Parker, L. A. (2021). Constituents of Cannabis Sativa. Advances in Experimental Medicine and Biology 1264:1-13.

Saurabh, V., Barman, K. and Singh, A. K. (2019). Synergistic effect of salicylic acid and chitosan on postharvest life and quality attributes of jamun (Syzygium cumini Skeels) fruit. Acta Physiologiae Plantarum 41:89.

Shiponi, S. and Bernstein, N. (2021). Response of medical cannabis (Cannabis sativa L.) genotypes to P supply under long photoperiod: functional phenotyping and the ionome. Industrial Crops and Products 161:113154.

Silva, A. R., Lima, G. S., Azevedo, C., Gheyi, H. R., Soares, L. A. and Veloso, L. (2022). Salicylic acid improves physiological indicators of soursop irrigated with saline water. Revista Brasileira de Engenharia Agrícola e Ambiental 26:412-419.

Singh, S. (2023). Salicylic acid elicitation improves antioxidant activity of spinach leaves by increasing phenolic content and enzyme levels. Food Chemistry Advances 2:100156.

Szczykutowicz, M. K., Dziurka, M., Blaževi, I., Đulović, A., Apola, A., Ekiert, H. and Szopa, A. (2022). Impacts of elicitors on metabolite production and antioxidant potential and tyrosinase inhibition in watercress microshoot cultures. Applied Microbiology and Biotechnology 106:1-15.

Thambavani, S. and Sabitha, M. A. (2011). The spectral determination of chlorophylls a, b and total carotenoids using various solvents for tree species growing near sugar mill. Asian Journal of Experimental Chemistry 7:5-9.

Thakur, M., Bhattacharya, S., Khosla, K. P. and Puri, S. (2019). Improving production of plant secondary metabolites through biotic and abiotic elicitation. Journal of Applied Research on Medicinal and Aromatic Plants 12:1-12.

Velioglu, G., Mazza, L. G. and Oomah, B. D. (1998). Antioxidant activity and total phenolics in selected fruits, vegetables and grain products. Journal of Agricultural and Food Chemistry 46:4113-4117.

Wang, W., Huang, W. and Xu, H. (2017). Effect of salicylic acid on the gene transcript and protein accumulation of flavonoid biosynthesis- related enzymes in Vitis vinifera cell suspension cultures. HortScience 52:1772-1779.

Wei, X., Vrieling, K., Kim, H. K., Mulder, P. P. J. and Klinkhamer P. G. L. (2021). Application of methyl jasmonate and salicylic acid lead to contrasting effects on the plant's metabolome and herbivory. Plant Science 303:110784.

Yusuf, M., Hayat, S., Alyemeni, M., Fariduddin, Q. and Ahmad, A. (2013). Salicylic acid: Physiological roles in plants. SALICYLIC ACID, pp.15-30.