Effects of Bacillus probiotics, Bacillus subtilis and Bacillus cereus dietary additional to controlling Vibriosis infection of white shrimp (Litopenaeus vannamei)

Main Article Content

Wongpracha, P.
Kanthawong, P.
Arun, J.
Jeyaraj, G. P.
Jongput, B.
Srijad, S.
Kitikiew, S.

Abstract

The effects of the Bacillus probiotics powder, Bacillus subtilis 7 x 106 cfu g-1 and Bacillus cereus 2.5 x 107 cfu g-1 via dietary additional to control vibriosis disease in white shrimp culture for 14 days were evaluated. The results indicated that good recovery in shrimp fed with probiotic (T4) and the mortality reduced (86.7%), in comparison with the group without probiotics and with pathogen, Vibrio spp. (T3). In addition, it presented lower counts of Vibrio spp. than group fed without the probiotic causing a significantly reduced pathogen. Bacillus probiotics, B. subtilis and B. cereus had a better effect on hematological shown in the total haemocyte counts (THC) of the group with probiotic and with pathogen, Vibrio spp. (T4) had the highest THC every day throughout the experiment experiment followed by the group with probiotic and without pathogen (T2) respectively.  Therefore,  B. subtilis and B. cereus significantly enhanced the shrimps' ability to recover from Vibrio spp. infections and enhanced the quality of their cultures.

Article Details

How to Cite
Wongpracha, P., Kanthawong, P., Arun, J., Jeyaraj, G. P., Jongput, B., Srijad, S., & Kitikiew, S. (2024). Effects of Bacillus probiotics, Bacillus subtilis and Bacillus cereus dietary additional to controlling Vibriosis infection of white shrimp (Litopenaeus vannamei). International Journal of Agricultural Technology, 20(1), 427–440. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/12265
Section
Original Study

References

Abdel-Latif, H. M. R., Yilmaz, E., Dawood, Mahmoud, A. O., Ringø, E., Ahmadifar, E. and Yilmaz, S. (2022). Shrimp Vibriosis and Possible Control Measures Using Probiotics, Postbiotics, Prebiotics, and Synbiotics: A Review. Aquaculture, 551:737-951.

Abdel-Tawwab, M., Khalil, R. H., Nour, A. M., Elkhayat, B. K., Khalifa, E. and Abdel-Latif, H. M. R. (2020). Effects of Bacillus subtilis-fermented rice bran on water quality, performance, antioxidants/oxidants, and immunity biomarkers of White leg shrimp (Litopenaeus vannamei) reared at different salinities with zero water exchange. Journal of Applied Aquaculture, 1-26.

Alderman, D. J. and Hastings, T. S. (1998). Antibiotic use in aquaculture: development of antibiotic resistance – potential for consumer health risks. International Journal of Food Science & Technology, 33:139-155.

Amatul-Samahah, M. A., Wan Omar, W. H. H., Mohd Ikhsan, N. F., Amal Azmai, M. N., Zamri-Saad, M. and Ina-Salwany, M. Y. (2020). Vaccination trials against vibriosis in shrimp: a review. Aquaculture Report, 18:100471.

Balcázar, J. L. and Rojas-Luna, T. (2007). Inhibitory Activity of Probiotic Bacillus subtilis UTM 126 Against Vibrio Species Confers Protection Against Vibriosis in Juvenile Shrimp (Litopenaeus vannamei). Current Microbiology, 55:409-412.

Bell, T. A. and Lightner, D. V. (1988). A Handbook of Normal Penaeid Shrimp Histology.World Aquaculture Society, Baton Rouge, LA.

Cabello, F. C. (2006). Heavy use of prophylactic antibiotics in aquaculture: a growing problem for human and animal health and for the environment. Environmental Microbiology, 8:1137-1144.

Cai, Y., Yuan, W., Wang, S., Guo, W., Li, A., Wu, Y. and Zhou, Y. (2019). In vitro screening of putative probiotics and their dual beneficial effects: to white shrimp (Litopenaeus vannamei) postlarvae and to the rearing water. Aquaculture, 498:61-71.

Chatterjee, S. and Haldar, S. (2012). Vibrio related diseases in aquaculture and development of rapid and accurate identification methods. Journal of Marine Science: Research & Development, 1:1-7.

FAO (2019). State of Fisheries and Aquaculture in the World. Rome, Italy, Food and Agriculture Organization of the United Nations. ISBN 9781424464968.

George, E. G. J., Jeyaraj, G. P. and Arumugham, V. R. (2018). Bio-confronting Efficacy of the Bacillus Probiotic Strains of NOVIB™ in Controlling Vibriosis on Low Saline Semi-Intensive Pond Culture System of the White Leg Shrimp, Litopenaeus vannamei (Boone, 1931). International Journal of Current Microbiology and Applied Sciences, 2639-2651.

Harlıoglu, M. M. and Farhadi, A. (2017). Feminization strategies in crustacean aquaculture. Aquaculture International, 25:1453-1468.

Heyman, M. and Menard, S. (2002). Probiotic microorganisms: how they affect intestinal pathophysiology. Cellular and Molecular Life Sciences, 59:1151-1165.

Hoseinifar, S. H., Sun, Y. Z., Wang, A. and Zhou, Z. (2018). Probiotics as means of diseases control in aquaculture, a review of current knowledge and future perspectives. Front. Microbiol, 9:24-29.

Huisakul, W., Limsuwan, C., Chuchird, N. and Panyachardruk, K. (2007). Effect of Lactobacillus spp. In Close Culture System of Black Tiger Shrimp. Proceedings of 45th Kasetsart University Annual Conference: Fisheries, 45:245-253.

Jayasree, L., Janakiram, P. and Madhavi, R. (2006). Characterization of Vibrio spp. Associated with diseased shrimp from culture ponds of Andhra Pradesh (India). Journal of the World Aquaculture Society, 37:523-532.

Kumar, V. and Roy, S. (2017). Aquaculture drugs: sources, active ingredients, pharmaceutic preparations and methods of administration. Journal of Aquaculture Research & Development, 8:510.

Limbu, S. M., Zhou, L., Sun, S. X., Zhang, M. L. and Du, Z. Y. (2018). Chronic exposure to low environmental concentrations and legal aquaculture doses of antibiotics cause systemic adverse effects in Nile tilapia and provoke differential human health risk. Environment International, 115:205-219.

Madhana, S., Kanimozhi, G. and Panneerselvam, A. (2021). Probiotics in Shrimp Aquaculture. In Advances in Probiotics, 309-325.

Mohammadi, G., Rafiee, G., Tavabe, K. R., Abdel-Latif, H. M. R. and Dawood, M. A. O. (2021). The enrichment of diet with beneficial bacteria (single- or multi- strain) in biofloc system enhanced the water quality, growth performance, immune responses, and disease resistance of Nile tilapia (Oreochromis niloticus). Aquaculture, 539: 736640.

Moullac, G., Groumellec, M. L., Ansquer, D., Froissard, S., Levy, P. and Aquacop. (1997). Haematological and phenoloxidase activity changes in the shrimp Penaeus stylirostrisin relation with the moult cycle: protection against vibriosis. Fish & Shellfish Immunology, 227-234.

Pattukumar, V., Kanmani, P., Yuvaraj, N., Paari, A. and Arul, V. (2013). Improved resistance to Vibrio parahaemolyticus in black tiger shrimp Penaeus monodon treated with Streptococcus phocae PI80 and Bacillus subtilis. Israeli Journal of Aquaculture – Bamidgeh, 1-10.

Pérez-Sánchez, T., Mora-Sánchez, B. and Balcázar, J. L. (2018). Biological approaches for disease control in aquaculture: advantages, limitations and challenges. Trends in Microbiology, 26:896-903.

Ringø, E. (2020). Probiotics in shellfish aquaculture. Aquaculture and Fisheries, 5:1-27.

Ringø, E., Van Doan, H., Lee, S. H., Soltani, M., Hoseinifar, S. H., Harikrishnan, R. and Song, S. K. (2020). Probiotics, lactic acid bacteria and bacilli: interesting supplementation for aquaculture. Journal of Applied Microbiology, 129:116-136.

Rodrýguez, J. and Moullac, G. L. (2000). State of the immunological tools and health control of penaeid shrimp. Aquaculture, 191:101-119.

Shinn, A. P., Pratoomyot, J., Griffiths, D., Trong, T. Q., Vu, N. U., Jiravanichpaisal, P. and Briggs, M. (2018). Asia shrimp production and the economic cost of disease. Asian Fisheries Science, 31:29-58.

Smith, P. (2008). Antimicrobial resistance in aquaculture. Revue scientifique et technique, 27:243-264.

Songsuk, A., Sungsirin, N. and Suwanmalee, P. (2018). Effect of probiotic Bacillus subtilis on control of Vibrio spp. and survival of pacific white shrimp (Litopenaeus vannamei) larvae. Khon Kaen Agriculture Journal, 1:961-967.

Sritunyalucksana, K., Gangnonngiw, W., Archakunakorn, S., Fegan, D. and Flegel, T. W. (2005). Bacterial clearance rate and a new differential hemocyte staining method to assess immunostimulant activity in shrimp. Diseases of Aquatic Organisms 63:89-94.

Van Hai, N. and Fotedar, R. (2010). A review of probiotics in shrimp aquaculture. Journal of Applied Aquaculture, 22:251-266.

Vaseeharan, B. and Ramasamy, P. (2003). Control of patho genic Vibrio spp. by Bacillus subtilis BT23, a possible probiotic treatment for black tiger shrimp Penaeus monodon. Letters in Applied Microbiology, 36:83-87.

Verschuere, L., Rombaut, G., Sorgeloos, P. and Verstraete, W. (2000). Probiotic bacteria as biological control agents in aquaculture. Microbiology and Molecular Biology Reviews, 64:655-671.

Vidal, J. M. A., PESSÔA, M. N. D. C., Santos, F. L. D., Mendes, P. D. P. and Mendes, E. S. (2018). Probiotic potential of Bacillus cereus Against Vibrio spp. In post-larvae Shrimps. International Standard Serial Number, 1983-2125.

Wang, Y. C., Hu, S. Y., Chiu, C. S. and Liu, C. H. (2019). Multiple-strain probiotics appear to be more effective in improving the growth performance and health status of white shrimp, Litopenaeus vannamei, than single probiotic strains. Fish & Shellfish Immunology, 84:1050-1058.

Zokaeifar, H., Babaei, N., Saad, C. R., Kamarudin, M. S., Sijam, K. and Balcazar, J. L. (2014). Administration of Bacillus subtilis strains in the rearing water enhances the water quality, growth performance, immune response, and resistance against Vibrio harveyi infection in juvenile white shrimp, Litopenaeus vannamei. Fish & Shellfish Immunology, 36:68-74.

Zorriehzahra, M. J., Delshad, S. T., Adel, M., Tiwari, R., Karthik, K., Dhama, K. and Lazado, C. C. (2016). Probiotics as beneficial microbes in aquaculture: an update on their multiple modes of action: a review. Veterinary Quarterly, 36:228-241.