Pathogenic and genetic characterization of Iranian isolates of Fusarium oxysporum f. sp. Lentis by ISSR analysis
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Abstract
Lentis (Lens culinaris Medik.) is an important widely cultivated food legume crop in Iran. The wilt disease caused by Fusarium oxysporum f.sp. lentis has been observed in high incidence rates during recent years(2007-2008) and has caused high losses in some of the cultivated areas. In order to study the pathogenicity and genetic diversity of Fusarium oxysporum f. sp. Lentis (Fol) isolates. Forty five isolates of Fusarium oxysporum f. sp. Lentis isolated from wilted lentis plants collected from different lentis growing areas in Iran, with two isolates from ICARDA were investigated. A pathogenicity test were performed for all isolates. Results showed that the Fol isolates differ in their aggressiveness on the susceptible lines could be grouped into 3 categories based on bayaa scales. The amount of genetic variation were evaluated by ISSR technique, using six primers, all of them could show the diversity between isolates. The most diversity was observed by primers (CAG)5 and ISSR10 (93%) and the minimal diversity by ISSR02 (50%). Six ISSR primers produced 156 fragments that the number of fragment was variable, ranging from 6 to 17 fragments in each profile. The analysis showed that F. oxysporum f .sp. lentis isolates could be differentiated into 6 groups at 74% similarity, and that could partially separate isolates based on their geographical regions. But could not found any correlation between this marker and pathogenesis grouping.
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References
Bayaa, B., Erskine, W. and Hamdi, A. (1995). Evalution of a wild lentis collection for resistance to vascular wilt. Genetic Resources and crop Evalution, 42: 231-235.
Bayraktar, H. and Dolar, F.S. (2008). Genetic Diversity of Wilt and Root Rot Pathogens of Chickpea, Turk Journal Agriculture, 33: 1-10.
Belabid, L. and Fortas, Z. (2002). Virulence and vegetative compatibility of Algerian isolates of Fusarium oxysporum f.sp. Lentis. Phytopathology Mediterr., 41: 179-187.
Belabid, L., Baum, M., Fortas, Z., Bouzand, Z., and Eujal, I. (2004). Pathogenic and genetic characterization of Algerian isolates if Fusarium oxysporum f.sp. Lentis by RAPD and AFLP analysis. African Journal of Biotechnology, 3: 25-31.
Beniwal, S.P.S., Bayaa, B., Weigand, S., Makkouk, K., and Saxena M.C. (1993). Field Guide to Lentis Diseases and insect Pests. International Centre for Agricultural Research in the Dry Areas, Aleppo, Syria, 106 pp.
Chaudhary, R.G., and Amarjit, K., (2002). Wilt disease as a cause of shift from lentis cultivation in Sangod Tehsil of Kota, Rajasthan. Indian Journal of Pulses Research, 15:193–194.
Khare, M.N. (1980). Wilt of Lentis. Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur, M.P India 155 pp.
Litt, M., and Luty, J.A. (1989). A hypervariable microsatellite revealed by in vitro amplification of dinucleotide repeat within the cardiac muscle actin gene. American Journal of Human Genetics, 44: 397-401.
Mbofung, G.Y.C. (2006). Phylogeny, molecular detection, and genetic variation of Fusarium oxysporum, vascular wilt pathogen of lettuce. Department of plant science. The University of Arizona. 202pp.
McCall, A.C., Kelly, D. and Chapman, H.M. (2004). Little geographic or host plant variation in a Chionochloa (Poaceae) seed predator (Cecidomydiidae: undescribed species). New Zealand Journal Ecology, 28: 215-224.
Meyer, W., Lieckfeldt, E., Kuhls, K., Freedman, E.Z., Borner, T. and Mitchell, T.G. (1993). DNA- and PCR-fingerprinting in fungi. In DNA fingerprinting: State of the Science (ed. S.D.J. Pena, R. Chakraborty, J.T. Epplen, and Agriculture Journal Jeffreys), pp. 311-320.
Thanos, M., Shönian, G., Meyer, W., Sweynoch, C., Gräser, y., Mitchell, T.G., Presber, W. And Tietz, H.J. (1996). Rapid identification of Candida species by DNA fingerprinting with PCR. Journal Clinical Microbiology, 34: 615-621.
Tosi, L., and Cappelli, C (2001). First report of Fusarium oxysporum f. sp. lentis of lentis in Italy. Plant Disease, 85: 562.
Setti, B., and Bouznad, Z. (1998). Fusarium root rot and wilt of lentis in Western Algeria. In Proceeding of the 3rd European Conference on Grain Legumes, 14-19 November, Valladolid, Spain, AEP (Eds), 254 p.
Yadav, S.S. McNeil, D.L. Stevenson. Ph. S. Lentis An Ancient Crop for Modern Times. (2007). P.O. Box 17, 3300 AA Dordrecht, the Netherlands. 443pp.
Zézé, A., Sulistyowat, E., Ophel-Keller, K., Barker, S. and Smith, S. (1997). Intersporal genetic variation of Gigaspora margarita, a vesicular arbuscular mycorrhizal fungus, revealed by M13 minisatellite-primed PCR. Applied Environment Microbiology, 63: 676-678.
