In vitro evaluation of antibacterial potential of Annona squamosa L. and Annona reticulata L. from Similipal Biosphere Reserve, Orissa, India
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Abstract
Three different solvent extracts of leaf of Annona squamosa L. and Annona reticulata L. Were studied for its antibacterial activity. Agar cup and broth dilution methods were selected to test antibacterial activity using three Gram-positive (Bacillus subtilis, Staphylococcus aureus and Staphylococcus epidermidis) and five Gram-negative (Escherichia coli and Pseudomonas aeruginosa, Salmonella typhi, Vibrio alginolyticus, Vibrio cholerae) bacteria. The screening results showed that highest inhibition was observed by the methanol extract followed by petroleum ether and aqueous extracts for both Annona squamosa and Annona reticulata leaf. Bacillus subtilis, Staphylococcus epidermidis, Staphylococcus aureus and Vibrio alginolyticus are the most sensitive bacterial strains among all test organisms. None of the plant extracts showed growth of inhibition against Salmonella typhi.
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