Dynamics of acid phosphatase production of the ectomycorrhizal mushroom Cantharellus tropicalis
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Abstract
Isolates of the ectomycorrhizal fungus Cantharellus tropicalis were grown in axenic culture to study the effect of pH, temperature, nitrogen source, carbon source, phosphorus source and heavy metals on the production of acid phosphatase and mycelial growth. The results of present study showed optimum mycelial growth with pH 4 at 15 and 35±2oC. The ectomycorrhizal mushroom mycelia utilized lactose and yeast extract as best carbon and nitrogen source for biomass production. Ferrous sulphate supported the maximum mycelial growth when different trace and heavy metal were tested. Among the phosphorus sources, di- Sodium hydrogen phosphate supported maximum growth. The acid phosphatase production did not follow a uniform pattern as inferred from the observations in this study. High biomass did not mean high acid phosphatase production. However, pH 5 at 15 and 35±2oC supported high enzyme production. Fries Das medium supplemented with Inositol and yeast extract produced maximum acid phosphatase in in vitro studies. Ferric chloride produced considerably higher acid phosphatase among different trace elements tested. The present study demonstrates various factors of acid phosphatase production, an important feature for selecting ectomycorrhizal mushrooms for field inoculations.
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