Meta-analysis of dietary supplementation with hemp products (Cannabis sativa L.) in broiler chicken: performances, blood profiles, intestinal morphology, and meat physicochemical parameters
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Abstract
The results showed that dietary hemp products reduced plasma triglycerides (SMD = -2.263; P < 0.01), cholesterol (SMD = -1.239; P < 0.05), and low-density lipoprotein (SMD = -5.711; P < 0.01), whereas the plasma high-density lipoprotein (SMD = 3.193; P < 0.01) and jejunal villus height (SMD = 4.536; P < 0.01) were increased. However, broiler performances (average daily feed intake, body weight, feed conversion ratio) and meat physicochemical parameters (pH, lightness, redness, yellowness, crude protein, and ether extract) were not significantly affected. Therefore, the study indicated that dietary hemp products in broiler chicken could improve blood profiles and gut health, suggesting their potential as an alternative feedstuff in broiler production.
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References
Alagawany, M., Elnesr, S. S., Farag, M. R., Abd El-Hack, M. E., Khafaga, A. F., Taha, A. E. and Dhama, K. (2019). Omega-3 and omega-6 fatty acids in poultry nutrition: effect on production performance and health. Animals, 9:573.
Callaway, J. C. (2004). Hemp seed as a nutritional resource: an overview. Euphytica, 140:65-72
DerSimonian, R. and Laird, N. (2015). Meta-analysis in clinical trials revisited. Contemporary clinical trials, 45:139-145.
EFSA (2011). Scientific Opinion on the safety of hemp (Cannabis genus) for use as animal feed. EFSA Journal, 9:1-41.
Eriksson, M. and Wall, H. (2012). Hemp seed cake in organic broiler diets. Animal Feed Science and Technology, 171:205-213.
Gowda, S., Desai, P. B., Hull, V. V., Avinash, A. K., Vernekar, S. N. and Kulkarni, S. S. (2009). A review on laboratory liver function tests. The Pan African Medical Journal, 3:17.
Gurevitch, J., Koricheva, J., Nakagawa, S. and Stewart, G. (2018). Meta-analysis and the science of research synthesis. Nature, 555:175-182.
Higgins, J. P., Thompson, S. G., Deeks, J. J. and Altman, D. G. (2003). Measuring inconsistency in meta-analyses. BMJ, 327:557-560.
Jing, M., Zhao, S. and House, J. D. (2017). Performance and tissue fatty acid profile of broiler chickens and laying hens fed hemp oil and HempOmegaTM. Poultry Science, 96:1809-1819.
Kanbur, G. (2022). Growth-depressing effect of dietary hempseed oil on broiler performance in the starting period and alterations in meat oxidation, serum parameters and abdominal fatty acids. Animal Science Papers and Reports, 40:203-216.
Karlsson, L., Finell, M. and Martinsson, K. (2010). Effects of increasing amounts of hempseed cake in the diet of dairy cows on the production and composition of milk. Animal, 4:1854-1860.
Khan, R. U., Durrani, F.-E.-R., Chand, N., Anwar, H., Naz, S., Farooqi, A. and Manzoor, M. N. (2009). Effect of Cannabis Sativa Fortified Feed on Muscle Growth and Visceral Organs in Broiler Chicks. International Journal of Biology and Biotechnology, 6:179-182.
Khan, R. U., Durrani, F. R., Chand, N. and Anwar, H. (2010). Influence of feed supplementation with cannabis sativa on quality of broilers carcass. Pakistan Veterinary Journal, 30:34-38.
Koricheva, J., Gurevitch, J. and Mengersen, K. (2013). Handbook of meta-analysis in ecology and evolution. New Jersey, Princeton University Press, pp. 3-14.
Mahmoudi, M., Farhoomand, P. and Nourmohammadi, R. (2015). Effects of different levels of hemp seed (Cannabis sativa L.) and dextran oligosaccharide on growth performance and antibody titer response of broiler chickens. Italian Journal of Animal Science, 14:114-119.
Mir, N. A., Rafiq, A., Kumar, F., Singh, V. and Shukla, V. (2017). Determinants of broiler chicken meat quality and factors affecting them: a review. Journal of food science and technology, 54:2997-3009.
Ogbuewu, I. P., Emenalom, O. O. and Okoli, I. C. (2015). Alternative feedstuffs and their effects on blood chemistry and haematology of rabbits and chickens: a review. Comparative Clinical Pathology, 26:277-286.
Ogbuewu, I. P., Okoro, V. M. and Mbajiorgu, C. A. (2020). Probiotic-yeast improves performance indicators in broiler chickens: evidence from meta-analysis. Applied Ecology and Environmental Research, 18.
Ogbuewu, I. P., Mabelebele, M. and Mbajiorgu, C. A. (2023). Meta-analysis of blood indices and production physiology of broiler chickens on dietary fermented cassava intervention. Tropical Animal Health and Production, 55:368.
Opyd, P. M., Jurgoński, A., Fotschki, B. and Juśkiewicz, J. (2020). Dietary hemp seeds more effectively attenuate disorders in genetically obese rats than their lipid fraction. The Journal of nutrition, 150:1425-1433.
Rasool, U. (2018). Safety and efficacy of hemp products in broiler production (Master Thesis). The University of Manitoba, Canada.
Rubio, L. A. (2019). Possibilities of early life programming in broiler chickens via intestinal microbiota modulation. Poultry science, 98:695-706.
Sana, B., Chand, N., Naz, S., Alhidary, I. A., Khan, R. U., Shamsi, S. and Tufarelli, V. (2024). Effects of hempseed (Cannabis sativa L.) in diet on growth, gut health, and immunity in broilers. Veterinary Quarterly, 44:1-10.
Skřivan, M., Englmaierová, M., Taubner, T. and Skřivanová, E. (2020). Effects of dietary hemp seed and flaxseed on growth performance, meat fatty acid compositions, liver tocopherol concentration and bone strength of cockerels. Animals, 10:458.
Small, E. (2015). Evolution and Classification of Cannabis sativa (Marijuana, Hemp) in Relation to Human Utilization. Botanical Review, 81:189-294.
Stillhart, C., Vučićević, K., Augustijns, P., Basit, A. W., Batchelor, H., Flanagan, T. R., ... and Müllertz, A. (2020). Impact of gastrointestinal physiology on drug absorption in special populations––An UNGAP review. European Journal of Pharmaceutical Sciences, 147:105280.
Sopian, Y., Sivapirunthep, P. and Chaosap, C. (2024). Effect of hempseed products on growth performance, carcass yield, omega-3, and omega-6 fatty acids in broiler: A meta-analysis. IOP Conference Series: Earth and Environmental Science, 1377:012081.
Stastnik, O., Jůzl, M., Karásek, F., Fernandová, D., Mrkvicová, E., Pavlata, L., Nedomová, Š., Vyhnánek, T., Trojan, V. and Doležal, P. (2019). The effect of hempseed expellers on selected quality indicators of broiler chicken ’ s meat. Acta Veterinaria Brno, 88:121-128.
Stastnik, O. N. D. R. E. J., Karasek, F. I. L. I. P., Stenclova, H. A. N. A., Burdova, E., Kalhotka, L., Trojan, V. and Mrkvicova, E. (2016). The effect of hemp by-products feeding on gut microbiota and growth of broiler chickens. MendelNet, 289-293.
Taubner, T., Skřivan, M., Englmaierová, M. and Malá, L. (2023). Effects of hemp seed and flaxseed on enzyme activity in the broiler chicken digestive tract. Animal, 17:100765.
Tufarelli, V., Losacco, C., Tedone, L., Passantino, L., Tarricone, S., Laudadio, V. and Colonna, M. A. (2023). Hemp seed (Cannabis sativa L.) cake as sustainable dietary additive in slow-growing broilers: effects on performance, meat quality, oxidative stability and gut health. Veterinary Quarterly, 43:1-12.
Vispute, M. M., Sharma, D., Mandal, A. B., Rokade, J. J., Tyagi, P. K. and Yadav, A. S. (2019). Effect of dietary supplementation of hemp (Cannabis sativa) and dill seed (Anethum graveolens) on performance, serum biochemicals and gut health of broiler chickens. Journal of Animal Physiology and Animal Nutrition, 103:525-533.
Vispute, M. M., Sharma, D., Biswas, A. K., Rokade, J. J., Chaple, A. R., Biswas, A. and Kapgate, M. G. (2021). Dietary hemp (Cannabis sativa L.) and dill seed (Anethum graveolens) improve physicochemical properties, oxidative stability, and sensory attributes of broiler meat. ACS Food Science and Technology, 1:453-461.
Vonapartis, E., Aubin, M. P., Seguin, P., Mustafa, A. F. and Charron, J. B. (2015). Seed composition of ten industrial hemp cultivars approved for production in Canada. Journal of Food Composition and Analysis, 39:8-12.
Wallace, B. C., Lajeunesse, M. J., Dietz, G., Dahabreh, I. J., Trikalinos, T. A., Schmid, C. H. and Gurevitch, J. (2017). Open MEE: Intuitive, open‐source software for meta‐analysis in ecology and evolutionary biology. Methods in Ecology and Evolution, 8:941-947.
Xu, Y., Li, J., Zhao, J., Wang, W., Griffin, J., Li, Y., Bean, S., Tilley, M. and Wang, D. (2021). Hempseed as a nutritious and healthy human food or animal feed source: a review. International Journal of Food Science and Technology, 56:530-543.