Association of TLR4 polymorphism with subclinical mastitis in early lactating riverine buffalo
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Abstract
Association of polymporphism of the TLR4 gene with the occurrence of subclinical mastitis in early lactating riverine buffaloes is determined. The buffalo samples TLR4 nucleotide sequence had 98% similarity with that of Bubalus bubalis from 96% to 97% homology with Bos taurus and Bos indicus. The buffalo samples showed a high degree of similarity in their amino acid sequences, averaging 96.2%, with those of the small ruminant species Ovis aries and Capra hircus. When compared to Bubalus bubalis, the translated amino acid sequences from the buffalo samples had an 80% similarity. However, the amino acid sequences of Bos indicus, Bos taurus, Ovis aries, and Capra hircus exhibited a lower similarity, averaging around 72%. Maximum likelihood algorithm phylogenetic analysis showed that the buffalo sample and Bubalus bubalis clade together, that separated from the clade of Bos indicus and Bos taurus which further separated with the clade of small ruminants. RFLP analysis showed three possible genotypes using RsaI restriction enzyme. Genotype A and B had a high odds ratio of 6.86 with significant association, there was 5.4 times greater probability that occurrence of subclinical mastitis can happen than genotype C. Therefore, genotypes A and B were found to be associated with the incidence of subclinical mastitis in buffaloes
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References
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