Effect of Chlorine Dioxide (ClO2) on Culture Medium Sterilization on Micropropagation of Persian Violet (Exacum affine Balf.f. ex Regel)

Article Sidebar

PDF
Published: Mar 15, 2018
Keywords:
Persian violet chlorine dioxide sterilization root induction flowering

Main Article Content

Srichuay, W.
Faculty of Science and Technology, Princess of Naradhiwas University, Narathiwat 96000, Thailand
Promchan, T.
Faculty of Agricultural Science and Technology, Silpakorn University, Petchaburee 71260, Thailand
Te-chato, S.
Department of Plant Science, Faculty of Natural Resources, Prince of Songkla University, Hatyai, Songkhla 90112, Thailand

Abstract

The effect of chlorine dioxide (ClO2) on culture medium sterilization in persian violet (Exacum affine Balf.f. ex Regel) has been studied. The culture medium was sterilized by ClO2, which promoted shoot formation. Culture medium supplemented with 5 ppm ClO2 gave the highest shoot at 9.27±4.13 shoot/explants. The explants were cultured on medium supplemented with 10 ppm ClO2 which induced the highest root formation at 62.50 % and 3.61±1.00 root/explants significant different with other treatments. In addition to flower induction, above treatment gave the highest flowering at 52.38 % and blooming flower at 14.28 %. In case of concentration of culture medium, ½ MS supplemented with 10 ppm ClO2 could be root induction at 100 % gave the highest number of root at 12.33±3.84 root/explants significant different with another treatment. The effect of agar concentration in medium, 0.60 % agar gave the best root induction at 94.44 % while 0.75% was induced flower at 52.38 % and blooming flower at 14.28 %. Another study is that effect of ClO2 when combination with silver nitrate (AgNO3) in micropropagation. The optimum concentration of AgNO3 to promoted root induction and flowering was 1.0 mg L-1 at 33.33% root induction, 58.33 % flowering, and 50.00 % blooming flower.

Article Details

How to Cite
Srichuay, W., Promchan, T., & Te-chato, S. (2018). Effect of Chlorine Dioxide (ClO2) on Culture Medium Sterilization on Micropropagation of Persian Violet (Exacum affine Balf.f. ex Regel). International Journal of Agricultural Technology, 14(2), 259–270. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/5895
Issue
Vol. 14 No. 2 (2018): March
Section
Original Study
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Ballal, S. K. (1990). Morphogenic potential of Exacum affine and Eustoma grandiflorum in tissue culture. Plant Growth Regulator Society of America Quarterly 18:73-76.

Beyer, E. M. (1976). A potent inhibitor of ethylene action in plants. Plant Physiology 58:268-271.

Cardoso, J. C. and Teixeira da Silva, J. A. (2012). Micropropagation of gerbera using chlorine dioxide (ClO2) to sterilize the culture medium. In Vitro Cellular & Developmental Biology - Plant 48:362-368.

Carrillo, A., Puente, M. E. and Bashan, Y. (1996). Application of diluted chlorine dioxide to radish and lettuce nurseries insignificantly reduced plant development. Ecotoxicology and Environmental Safety 35:57-66.

Ceasar, S. A., Maxwell, S. L., Prasad, K. B., Karthigan, M. and Ignacimuthu, S. (2010). Highly efficient shoot regeneration of Bacopa Monnieri (L.) using a two-stage culture procedure and assessment of genetic integrity of micropropagated plants by RAPD. Acta Physiologiae Plantarum 32:443-52.

Chen, Z. and Zhu, C. (2011). Combined effects of aqueous chlorine dioxide and ultrasonic treatments onpostharvest storage quality of plum fruit (Prunussalicina L.). Postharvest Biology and Technology 61:117-123.

Chithra, M., Martin, K. P., Sumandakumari, C. and Madhusoodanan, P. V. (2004). Silver nitrate induced rooting and flowering in vitro on rare rhoeophytic woody medicinal plant, Rotula aquatica Lour. Indian journal of Biotechnology 3:418-421.

Fadel, D., Kintzios, S., Economou, A. S., Moschopoulou, G. and Constantinidou, H. I. A. (2010). Effect of different strength of medium on organogenesis, phenolic accumulation and antioxidant activity of spearmint (Mentha spicata I.) The Open Horticulture Journal 3:31-35.

Fei, S., Read, P. E. and Riordan, T. P. (2000). Improvement of embryogenic callus induction and shoot regeneration of buffalograss by silver nitrate. Plant Cell, Tissue and Organ Culture 60:197-203.

Gantait, S., Mandal, N., Bhattacharyya, S. and Das, P. K. (2011). Induction and identification of tetraploids using in vitro colchicines treatment of Gerbera jamesonii Bolus cv. Sciella. Plant Cell, Tissue and Organ Culture 106:485-493.

Gonçalves, S., Fernandes, L. and Romano, A. (2010). High-Frequency in Vitro Propagation of the Endangered Species Tuberaria Major. Plant Cell, Tissue and Organ Culture 101:359-63.

Han, Y., Sherman, D. M., Linton, R. H., Nielsen, S. S. and Nelson, P. E. (2000). The effects of washing and chlorinedioxide gas on survival and attachmentof Escherichia coli O157: H7 to green pepper surfaces. FoodMicrobiology 17:521-533.

Huang, J., Wang, L., Ren, N., Ma, F. and Ma, J. (1997) Disinfection effect of chlorine dioxide on bacteria in water. Water Research 31:607-613.

Kannan, P., Premkumar, A. and Ignacimuthu. (2007). Thidiazuron induced shoot regeneration in the endangered species, Exacum travancoricum Beedi. Indian Journal of Biotechnology 6:564-566.

Kapchina-Toteva, V. M., Iakimova, E. T. and Chavdarov, I. P. (2005). Proceedings of the Balkan Scientific Conference of Biology in Plovdiv (Bulgaria). 19-21 May 2005. pp. 714-722.

Kumar, V., Parvatam, G. and Ravishankar, G. A. (2009). AgNO3-a potential regulator of ethylene activity and plant growth modulator. Journal of Biotechnoligy 2:1-15.

López-Velasco, G., Tomás-Calleja, A., Sbodio, A., Artés-Hernández, F. and Suslow, T. V. (2012). Chlorine dioxide dose, water quality and temperature affect the oxidative statusof tomato processing water and its ability to inactivate Salmonella. Food Control 26: 28-35.

Ma, J. H., Yao, J. L., Cohen, D. and Morris, B. (1998). Ethylene inhibitors enhance in vitro root formation from apple shoot cultures. Plant Cell Reports 17:211-214.

Murashige, T. and Skoog, F. (1962). A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiologia Plantarum 15:473-497.

Mohan, R., Chui, E. A., Biasi, L. A. and Soccol, C. R. (2005). Alternative in vitro propagation: use of sugarcane bagasse as a low cost support material during rooting stage of strawberry Cv. Dover. Brazilian Archives of Biology and Technology 48:37-42.

Ørnstrup, H., Mølgaard, J. P. and Farestveit, H. (1993), Somatic embryogenesis and plant regeneration from cell suspensions of Exacum affine. Plant Cell, Tissue and Organ Culture 35:37-41.

Peeters, J. E., Mazás, A. E., Masschelein, W. J., de Maturana, V. M. and Debacker, E. (1989) Effect of disinfection of drinking water with ozone or chlorine dioxide on survival of Cryptosporidium parvum oocysts. Applied and Environmental Microbiology 55:1519-1522.

Pratheesh, P. T. and Kumar, M. A. (2012). In vitro flowering in Rosa indica L. International Journal of Phamacy and Biological Science 2:196-200.

Sanputawong, S. and Te-chato, S. (2008). Effect of genotypes of oil palm as indicator for speed of callus and embryogenic callus formation. Journal of Agricultural Technology 4:147-156.

Sharma, A., Kumar, V., Giridhar, P. and Ravishankar, G. A. (2008). Induction of in Vitro Flowering in Capsicum frutescens under the Influence of Silver Nitrate and Cobalt Chloride and Pollen Transformation. Electronic Journal of Biotechnology 11:1-6.

Sivanesan, I., Song, J. Y., Hwang, S. J. and Jeong, B. R. (2011). Micropropagation of Cotoneaster wilsonii Nakai—a rare endemic ornamental plant. Plant Cell, Tissue and Organ Culture105:55-63.

Sirisom, Y. and Te-chato, S. (2014). Assessment of somaclonal variations of in vitro-plants derived from nodal culture of rubber trees by SSR markers. Songklanakarin Journal of Plant Science 1:7-12.

Srichuay, W. and Te-chato, S. (2014). Effect of chlorine dioxide (ClO2) on sterilization in micropropagation of pineapple cv. Phulae by bioreactor system. Khon Kaen Agriculture Journal 42(Suppl 3):75-80.

Šušek, A., Javornik, B. and Bohanec, B. (2002). Factors affecting direct organogenesis from flower explants of Allium giganteum. Plant Cell, Tissue and Organ Culture 68:27-33.

Suthar, R. K., Habibi, N. and Purohit, S. D. (2011). Influence of agar concentration and liquid medium on in vitro propagation of Boswellia serrata Roxb. Indian Journal of Biotechnology 10:224-227.

Taylor, G. R. and Butler, M. (1982). A comparison of the virucidal properties of chlorine, chlorine dioxide, bromine chloride and iodine. Journal of Hygiene 89:321-328.

Taylor, R. H., Falkinham, J. O., Norton, C. D. and LeChevallier, M. W. (2000). Chlorine, chloramine, chlorine dioxide, and ozone susceptibility of Mycobacterium avium. Applied and Environmental Microbiology 66:1702-1705.

Thawaro, S. and Te-chato, S. (2010). Effect of culture medium and genotype on germination of hybrid oil palm zygotic embryos. ScienceAsia 36:26-32.

Torres, A. C., Caldas, L. S. and Buso, J. A. (1998). Cultura de tecidos e transformação genética de plantas (Tissue culture and genetic transformation of plants). EMBRAPA-SPI and CNPH, Brasilia.

Wilson, S. C., Wu, C., Andriychuk, L. A., Martin, J. M., Brasel, T. L., Jumper, C. A. and Straus, D. C. (2005). Effect of chlorine dioxide gas on fungi and mycotoxins associated with sick building syndrome. Applied Environment Microbiology 71:5399-5403.

Yenchon, S. and S. Te-chato. (2014). Enhanced efficiency of flowering of Rose (Rosa hybrida) cv. “My Valentine” in vitro. Songklanakarin Journal of Plant Science 1:31.34.

Yuan, Y. and Xu, D. Q. (2001). Stimulation effect of gibberellic acid short-term treatment on leaf photosynthesis related to the increase in Rubisco content in board bean and soybean. Photosynthesis Research 68:39-47.