Production of Indole Acetic Acid (IAA) by Serratia marcescens subsp. marcescens and Rhodococcus aff. qingshengii
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Abstract
In vitro screening and characterization assay on indole acetic acid (IAA) production generally provides a reliable base for selection of useful plant growth promoting bacteria. Characterization at different parameters such as temperature, pH, and other physiological conditions is useful in finding optimum condition for the IAA production by bacteria. In this study, six endophytic bacterial isolates from Cocos nucifera L. and two strains of Rhodococcus aff. qingshengii were examined for their IAA production activity at different pH, temperature, and L-tryptophan concentration. Identification of effective IAA-producing bacteria was conducted by molecular phylogenetic analysis based on nucleotide sequences generated from 16S rRNA gene. The results showed that highest IAA concentration was produced by S. marcescens subsp. marcescens strain KB01, S. marcescens subsp. marcescens strain KB05, and R. aff. qingshengii strain 100A with 64.75 µg/mL, 56.60 µg/mL, and 18.06 µg/mL IAA, respectively. The IAA production by these bacteria was affected by L-tryptophan concentrations and was optimum at basic condition (pH 8-9).
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