Chromosome complement and developmental competence of swamp buffalo oocytes matured and fertilized in vitro

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Published: Jan 16, 2015
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Chromosome Oocytes Embryos In vitro

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Ocampo, M. B.
Philippine Carabao Center, Reproductive Biotechnology Unit, Science City of Munoz, 3120 Nueva Ecija, Philippines
Manuel, J. A.
Philippine Carabao Center, Reproductive Biotechnology Unit, Science City of Munoz, 3120 Nueva Ecija, Philippines
Soriano, R. P.
Philippine Carabao Center, Reproductive Biotechnology Unit, Science City of Munoz, 3120 Nueva Ecija, Philippines
Ocampo, L. C.
Philippine Carabao Center, Reproductive Biotechnology Unit, Science City of Munoz, 3120 Nueva Ecija, Philippines

Abstract

The chromosome complement of swamp buffalo (SB) oocytes matured in vitro and competence of resulting zygotes to the blastocyst stage based on the timing of its appearance and its implications has been discussed. In Expt 1, chromosomal aneuploidy was found more prevalent in oocytes cultured in maturation conditions without pregnant mare serum gonadotropin (PMSG) addition. The completion of 1st meiosis/nuclear maturation does not always guarantee the presence of complete chromosome complement of oocytes maturing in vitro. In Expt. 2, diploidy was the result of normal fertilization while polyploidy arised from polyspermic fertilization. In Expt. 3, the blastocyst formation rate of early- cleaving embryos was significantly higher than late- cleaving embryos supporting the idea that early- cleaving embryos are more likely to have normal chromosome complement resulting from normal fertilization than late- cleaving embryos. The results provide some insight into several important aspect of SB fertilization in vitro and could be used as a criterion in the production and eventual selection of embryos for embryo transfer.

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Ocampo, M. B., Manuel, J. A., Soriano, R. P., & Ocampo, L. C. (2015). Chromosome complement and developmental competence of swamp buffalo oocytes matured and fertilized in vitro. International Journal of Agricultural Technology, 11(1), 57–68. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/5998
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Vol. 11 No. 1 (2015): January
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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