Identification of endophytic fungi associated in banana (Musa paradisiaca L.) and evaluation of its enzymatic abilities
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Abstract
Various microorganisms such as fungal endophytes live in Musa paradisiaca that grow in internal tissues without harming and causing any diseases on its host plant. These fungi are known for their ability to produce extracellular enzymes. In this study, nine fungal endophytes were identified through cultural, morphological and DNA sequencing of the ITS1 and ITS4 regions. These were Cladosporium cladosporioides with 99.14% identity, Fusarium chlamydosporium with 99.80% identity, Fusarium keratoplasticum with 98.46% identity, Fusarium solani strain f2-f6 with 98.87% identity, Fusarium solani strain ZB11263612 with 98.07% identity, Fusarium solani strain F10-3 with 99.24% identity, Geotrichum candidum with 96.32% identity, Nigrospora oryzae with 100.00% identity and Schizophyllum commune with 99.66% identity. Eight out of nine fungi were able to degrade starch. The endophyte F. solani strain f2-f6 obtained highest enzymatic index of 14.16 in amylase production. On the other hand, four out of nine fungal endophytes were able to show cellulolytic activity. N. oryzae obtained highest enzymatic index of 13.13 in cellulase activity. Lastly, only F. solani strain f2-f6 showed color change for laccase activity. Thus, the study revealed that fungal endophytes from M. paradisiaca have the ability to produce these different kinds of enzymes that are of industrial and biotechnological importance
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