Live birth after artificial insemination using cryopreserved epididymal sperm recovered from the cauda epididymis of slaughtered non-descript bucks in the Philippines
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Abstract
The study determined the viability of cryopreserved epididymal sperm (EpS) to full term pregnancy through AI technique in hormone responsive recipient goats. Cryopreserved EpS with post thaw motility score of ≥20% were used to inseminate estrus-synchronized (ES-AI) and ovulation-synchronized (FT-AI) recipient goats. The ES-AI goats had a mean hormone response rate (HRR) of 55.5% with a Pregnancy rate (PR) of 25% and full term delivery (FTD) rate of 20%. On the other hand, the ovulation synchronized FT-AI goats registered a mean HRR of 75%, with a PR of 58% and FTD rate of 54.2%. The use of either GnRH or Chorionic gonadotropin on day 9 in the FT-AI goats resulted in a mean hormone response rate, pregnancy rate and full term delivery rate of 72.2%, 61.2%, 58.3 % and 78.6%, 54.5% and 54.5%, respectively. These results validated the usefulness of cryopreserved EpS as an alternative source of germplasm for cryopreservation and utilization for offspring production. Moreover, the study can serve as a research platform for germplasm conservation in vitro and in vivo of genetically diverse native ruminant farm animals as well as endangered wildlife ruminants endemic in the Philippines through the use of Assisted Reproductive Technologies (ARTs).
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References
Al Yacoub, A. N., Gauly, M., Sohnrey, B. and Holtz, W. (2011). Fixed time deep uterine insemination in PGF2α –synchronized goats. Theriogenology, 76:1730-1735.
Amiridis, G. S. and Cseh, S. (2012). ARTs in the reproductive management of small ruminants. Animal Reproduction Science, 130:152-161.
Atabay, E. P. (2015). Synchronization of ovulation and fixed time AI in water buffaloes: Recent development and insights on Philippine initiatives. Paper presented during the International Training Course on Dairy Herd Improvement by the Use of Reproductive Biotechnologies, Philippine Carabao Center, Science City of Munoz, Nueva Ecija, Philippines, July 13 to 22, 2015.
Atabay, E. C., Atabay, E. P., Maylem, E. R. S., Encarnacion E. C. and Salazar R. L. (2020). Enhancing prostaglandin based estrus synchronization protocol for artificial insemination in water buffaloes. Buffalo Bulletin, 39:53-60.
Beltran, M. A. G., Atabay, E. P., Atabay, E. C., Cruz, E. M., Aquino, F. P. and Cruz, L. C. (2013). Optimized extenders for cryopreservation of buck semen for artificial insemination. Philippine Journal of Veterinary and Animal Science, 39:1-10.
Blash, S., Melican, D. and Gavin, W. (2000). Cryopreservation of epididymal sperm obtained at necropsy from goats. Theriogenology, 54:899-905.
Bowdridge, E. C., Knox, W. B., Whisnant, C. S. and Farin, C. E. (2013). NCSynch: A novel rogestogen-free protocol for ovulation synchronization and timed AI in goats.Small Ruminant Research, 110:42-45.
Bumanlag, A. C., Harada, H. M., Divina, C. C., Ocampo, M. B. and Ocampo, L. C. (2017). Sperm Motility assessment of epididymal sperm from post mortem goat testicles held at 5°C. Entomology and Applied Science Letters, 4:16-18.
Cseh, S., Faigl, V. and Amiridis, G. S. (2012). Semen processing and artificial insemination in health management of small ruminants. Animal Reproduction Science, 130:187-192.
Fonesca, J. F., Maffili, V. V., Rodrigues, M. T., Santos, A. D., Rovay, H., Pinto, N. A., Brandao, F. Z. and Torres, C. A. (2015). Effects of hCG on progesterone concentrations and fertility in cyclic lactating Alpine goats. Animal Reproduction, 3:410-414.
Gautane, J. J., Balagan, E. J. Y., Manaois II, F. V., Ocampo, M. B. and Ocampo, L. C. (2016). Characteristics of epididymal sperm recovered from slaughterhouse derived testes in non-descript/ native goats in the Philippines. International Journal of Agricultural Technology, 12:215-228.
Guerrero, C. A., Gentry, G. Jr., Saenz, J., Bondioli, K. R. and Godke, R. A. (2008). Birth of calves after artificial insemination with cryopreserved bovine epididymal spermatozoa harvested from postmortem bulls. Reproduction, Fertility and Development, 21:105-105.
Holtz, W. B., Sohnrey, B., Gerland, M. and Driancourt, M. A. (2008). Ovysynch synchronization and fixed-time insemination in goats. Theriogenology, 69:785-792.
Hori, T., Ichikawa, M., Kawakami, E. and Tsutsui, T. (2004). Artificial insemination of froze epididymal sperm in beagle dogs. Journal of Veterinary Medical Science, 66:37-41.
Kikuchi, K., Kashiwazaki, N., Nagai, T., Noguchi, J., Shimada, A., Takahashi, R., Hirabayashi, M., Shino, M., Ueda, M. and Kaneko, H. (1999). Reproduction in pigs using frozen-thawed spermatozoa from epididymis stored at 4°C. Journal of Reproduction and Development, 54:345-350.
Kozdrowski, R., Nizanski, W. and Olech, O. (2011). Possibilities of using European bison (Bison bonasus) epididymal spermatozoa collected post-mortem for cryopreservation and artificial insemination: a pilot study. Reproductive Biotechnology and Endocrinology, 9:1-6.
Mamuad, F. V., Venturina, E. V. and H. Saito (2004). Collection, Processing and Handling of Buffalo Semen Manual. Philippine Carabao Center at CLSU. Water Buffaloes and Beef Cattle Improvement Project Japan International Cooperation Agency (JICA).
Moreno, J. S., Diaz, A. T., Pastor, A. P., Brunet, G. A. and Sebastian, A. L. (2006). Birth of live Spanish Ibex derived from artificial insemination with epididymal sperm retrieved after death. Theriogenology, 66:283-291.
Ocampo, L. C., Seralde, R., Dela Rosa, I. C. J., Lofranco, J. O., Ocampo, M. B. and Aquino, F. P. (2019a). Goat epididymal sperm survivability and fertility after storage at cryogenic temperature. Thai Journal of Veterinary Medicine, 49:53-56.
Ocampo, M. B., Averilla, M. A. J. M., Salinas, M. B. S. and Ocampo, L. C. (2019b). Quality and fertilizing ability of epididymal sperm from butchered Philippine carabao (Bubalus bubalis carabanensis). Thai Journal of Veterinary Medicine, 49:145-149.
Ocampo, M. B., Silvestre, J. Q., Viernes, V. D. and Ocampo, L. C. (2016). Live birth after transfer of vitrified embryos from superovulated goats. Research Opinions on Animal and Veterinary Science, 6:47-52.
Ocampo, M. B. and Ocampo, L. C. (2015). Strategies to improve the developmental competence of water buffalo oocytes in vitro. International Journal of Agricultural Technology, 11:2309.
Okazaki, T., Akiyoshi, T., Kan, M., Mori, M., Teshima, H. and Shimada, M. (2012). Artificial Insemination with seminal plasma improves the reproductive performance of frozen-thawed boar epididymal sperm. Journal of Andrology, 3:990-998.
Parmar, C. P., Dhami, A. J., Patel, J. A. and Belsare, V. P. (2020). Efficiency of different estrus synchronization protocols in Surti goats. Indian Journal of Veterinary Science and Biotechnology, 15:21-23.
Pujar, C., Puro, N. A., Markandenya, N. M., Mali, A. B. and Kumawat, B. L. (2016). Studies on the efficacy of Selectsynch and Ovsynch protocols for induction and synchronization of estrus in Osmanabadi goats. International Journal of Science, Environment and Technology, 5:4069- 4073.
Rahman, A. N. M. A., Abudullah, R. B. and Khadijah, W. E. W. (2008). A review of reproductive biotechnologies and their application in goat. Biotechnology, 7:371-384.
Saenz, J. R., Guerrero, C. A., Paccamonti, D., Eilts, B., Bondioli, K. R. and Godke, R. A. (2007). Processing of post mortem bovine epididymal sperm after cooling the testes for 24 hours. Reproduction, Fertility and Development, 20:126-127.
Sumeldan, J. D., Ocampo, L. C., Atabay, E. P., Celestino, E. F., Lazaro, J. V. and Ocampo, M. B. (2016). Comparison on the efficiency of estrus synchronization methods for artificial insemination in goats. International Journal of Agricultural Technology, 11:2489-2497.
Yulnawati, Y., Maheshwari, H., Rizal, M. and Boediono, A. (2013). The success rate of artificial insemination using post thawed spotted buffaloes epididymal sperm. Media Peternakan, 36:101-105.
Vallet, J. C., Baril, G., Leboeuf, B. and Perrin, J. (1992). Insemination artificielle intrauterine sous sontrole laparoscopique chez lespetits ruminants domestiques. Annals of Zootechnology, 41:305-309.
Whitley, N. C. and Jackson, D. J. (2004). An update on estrus synchronization in goats: A minor species. Journal of Animal Science, 82:270-276.
Zeleke, M., Assefa, H., Tegegne, A. and Muluneh, D. (2015). Estrus response and ertility of Menz and crossbred ewes to single prostaglandin injection protocol. Tropical Animal health and production, 48:53-57.