Collections, isolations, morphological study of Exserohilum turcicum and screening resistant varieties of corn to northern corn leaf blight disease
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Abstract
Collections of Northern Corn Leaf Blight (NCLB) samples were accomplished from 11 and 6 provinces in the north and northeast of Thailand, respectively, from which 478 isolates were collected. Pure cultures of the pathogen were obtained using the single spore isolation technique. Morphological study was performed by observing mycelia and conidia under a compound microscope. Characteristics of the fungus found in this study were similar to Exserohilum turcicum (Pass.) Leonard and Suggs, the causal agent of Northern Corn Leaf Blight disease. The highly virulent H1 and KL1 isolates identified through a pathogenicity test were selected for the study of growth and sporulation on eight media; CMA, CLIA, MEA, OMA, PDA, PDYeA, RBA and V-8 agar, under laboratory condition. Screening of corn varieties, resistant to NCLB disease, was carried out on 160 varieties in the field. The results revealed that nine varieties were highly resistant at level 1 to E. turcicum isolate H1 and KL1 in both vegetative and reproductive stages, namely IAS CO 6, SW05D-D10 199-36, SW05D-C8 1055-11, Syngenta 1, Syngenta 2, Syngenta 3, Syngenta 4, Syngenta 5 and Syngenta 6.
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