Extraction and antioxidant activities of broken Ganoderma lucidum spore

Article Sidebar

PDF
Published: Nov 15, 2021
Keywords:
Ganoderma lucidum Broken spores Microwave-assisted extraction Antioxidant activity

Main Article Content

Senphan, T.
Program in Food Science and Technology, Faculty of Engineering and Agro-Industy, Maejo University, Chiang Mai, Thailand
Takeungwongtrakul, S.
Department of Agricultural Education, School of Industrial Education and Technology, King Mongkut's Institute of Technology Ladkrabang, Ladkrabang, Bangkok, Thailand
Kaewthong, P.
School of Food Industry, King Mongkut's Institute of Technology Ladkrabang, Bangkok, Thailand

Abstract

Soxhlet and microwave-assisted extraction (MAE) methods showed the highest extraction yield and total phenolic content of extract from broken spores of Ganoiderma lucidum (p<0.05). However, the extraction time of MAE method was shorter than the soxhlet extraction method. The broken spores of G. Lucidum were extracted using different solvent types with MAE method. Ethanol extraction rendered extract with the highest total phenolic content and ferric reducing antioxidant power (FRAP) value (p<0.05). For metal chelating activity, ethanol and hexane extracts were significantly high compared to other extracts of broken G. lucidum spores (p<0.05). Thus, the use of appropriated extraction method and solvent type rendered to extract with high total phenolic content and antioxidant activities.

Article Details

How to Cite
Senphan, T., Takeungwongtrakul, S., & Kaewthong, P. (2021). Extraction and antioxidant activities of broken Ganoderma lucidum spore. International Journal of Agricultural Technology, 17(6), 2303–2316. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6392
Issue
Vol. 17 No. 6 (2021): November
Section
Original Study
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

AOAC (2000). Official methods of analysis. Association of Official Analytical Chemists, Washington, DC.

Aspé, E. and Fernández, K. (2011). The effect of different extraction techniques on extraction yield, total phenolic, and anti-radical capacity of extracts from Pinus radiata Bark. Industrial crops and products, 34:838-844.

Barbieri, A., Quagliariello, V., Del Vecchio, V., Falco, M., Luciano, A., Amruthraj, N. J. and Iaffaioli, R. V. (2017). Anticancer and anti-inflammatory properties of Ganoderma lucidum extract effects on melanoma and triple-negative breast cancer treatment. Nutrients, 9:1-9.

Benzie, I. F. F. and Strain, J. J. (1999). Ferric reducing/antioxidant power assay: Direct measure of total antioxidant activity of biological fluids and modified version for simultaneous measurement of total antioxidant power and ascorbic acid concentration. Methods in Enzymology, 299:15-27.

Bernard, L. K., David, S. K., Mark, N. O., Charles, M. K., Geoffrey, R. M., Charles, M., Laban, I. and Willy, T. K. (2012). Larvicidal action of extracts from Tithonia diversifolia against the dengue mosquito Aedes aegypti (Diptera: Culicidae). Journal of Biologically Active Products from Nature, 2:46-49.

Buamard, N. and Benjakul, S. (2015). Improvement of gel properties of sardine (Sardinella albella) surimi using coconut husk extracts. Food Hydrocolloids, 51:146-155.

Cör, D., Knez, Ž. and Knez Hrnčič, M. (2018). Antitumour, antimicrobial, antioxidant and antiacetylcholinesterase effect of Ganoderma lucidum terpenoids and polysaccharides: A review. Molecules, 23:1-21.

Cui, N., Zhang, L., Quan, M. and Xu, J. (2020). Profile of the main bioactive compounds and in vitro biological activity of different solvent extracts from Ginkgo biloba exocarp. RSC Advances, 10:45105-45111.

Dinis, T., Madeira, V. and Almeida, T. (1994). Action of phenolic derivatives (acetaminophen, salicylate and 5-aminosalicylate) as inhibitors of membrane lipid peroxidation and peroxyl radical scavengers. Archives of Biochemistry and Biophysics, 315:161-165.

Fukuzawa, M., Yamaguchi, R., Hide, I., Chen, Z., Hirai, Y., Sugimoto, A., Yasuhara, T. and Nakata, Y. (2008). Possible involvement of long chain fatty acids in the spores of Ganoderma lucidum (Reishi Houshi) to its anti-tumor activity. Biological and Pharmaceutical Bulletin, 31:1933-1937.

Gordy, W., Smith, W. V. and Trambarulo, R. F. (1966). Microwave spectroscopy. Dover Publications.

Heleno, S. A., Barros, L., Martins, A., Queiroz, M. J. R., Santos-Buelga, C. and Ferreira, I. C. (2012). Fruiting body, spores and in vitro produced mycelium of Ganoderma lucidum from Northeast Portugal: A comparative study of the antioxidant potential of phenolic and polysaccharidic extracts. Food Research International, 46:135-140.

Hsu, S. C., Ou, C. C., Li, J. W., Chuang, T. C., Kuo, H. P., Liu, J. Y., Chen, C. S., Lin, S. C., Su, C. H. and Kao, M. C. (2008). Ganoderma tsugae extracts inhibit colorectal cancer cell growth via G2/M cell cycle arrest. Journal of Ethnopharmacology, 120:394-401.

Intharuksa, A., Tipdaungta, P., Sirisa-ard, P., Vejabhikul, S. and Chansakaow, S. (2010). Sporoderm-breaking process for spores of Ganoderma lucidum (Leyss. ex Fr.) Karst. by broken-sporoderm machine and ball mill. Journal of Traditional Thai and Alternative Medicine, 8:199-204.

Jagtap, U. B., Panaskar, S. N. and Bapat, V. A. (2010) Evaluation of antioxidant capacity and phenol content in Jackfruit (Artocarpus heterophyllus Lam.) fruit pulp. Plant Foods for Human Nutrition, 65:99-104.

Lin, Z. b. and Zhang, H.N. (2004). Anti-tumor and immunoregulatory activities of Ganoderma lucidum and its possible mechanisms. Acta Pharmacologica Sinica, 25:1387-1395.

Ma, J., Fu, Z., Ma, P., Su, Y. and Zhang, Q. (2007). Breaking and characteristics of Ganoderma lucidum spores by high speed entrifugal shearing pulverizer. Journal Wuhan University of Technology, Materials Science Edition, 22:617-621.

Ma, Y. A., Cheng, Y. M., Huang, J. W., Jen, J. F., Huang, Y. S. and Yu, C. C. (2014). Effects of ultrasonic and microwave pretreatments on lipid extraction of microalgae. Bioprocess and Biosystems Engineering, 37:1543-1549.

Mandal, V., Mohan, Y. and Hemalatha, S. (2007). Microwave assisted extraction—an innovative and promising extraction tool for medicinal plant research. Pharmacognosy Reviews, 1:7-18.

Mohsin, M., Negi, P. and Ahmed, Z. (2011). Determination of the antioxidant activity and polyphenol contents of wild Lingzhi or Reishi medicinal mushroom, Ganoderma lucidum (W. Curt. Fr.) P. Karst. (higher Basidiomycetes) from central Himalayan hills of India. International Journal of Medicinal Mushrooms, 13:535-544.

Nantitanon, W., Yotsawimonwat, S. and Okonogi, S. (2010). Factors influencing antioxidant activities and total phenolic content of guava leaf extract. LWT-Food Science and Technology, 43:1095-1103.

Nile, S. H., Nile, A. S. and Keum, Y. S. (2017). Total phenolics, antioxidant, antitumor, and enzyme inhibitory activity of Indian medicinal and aromatic plants extracted with different extraction methods. 3 Biotech, 7:1-10.

Orole, O. O. (2016). GC-MS evaluation, phytochemical and antinutritional screening of Ganoderma lucidum. Journal of Advances in Biology and Biotechnology, 5:1-10.

Osorio-Tobón, J. F. (2020). Recent advances and comparisons of conventional and alternative extraction techniques of phenolic compounds. Journal of Food Science and Technology, 57:4299-4315.

Rafi, M., Meitary, N., Septaningsih, D. A. and Bintang, M. (2020). Phytochemical profile and antioxidant activity of Guazuma ulmifolia leaves extracts using different solvent extraction. Indonesian Journal of Pharmacy, 13:171-180.

Rani, P., Lal, M. R., Maheshwari, U. and Krishnan, S. (2015). Antioxidant potential of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Higher Basidiomycetes) cultivated on Artocarpus heterophyllus sawdust substrate in India. International Journal of Medicinal Mushrooms, 17:1171-1177.

Rezaie, M., Farhoosh, R., Iranshahi, M., Sharif, A. and Golmohamadzadeh, S. (2015). Ultrasonic-assisted extraction of antioxidative compounds from Bene (Pistacia atlantica subsp. mutica) hull using various solvents of different physicochemical properties. Food Chemistry, 173:577-583.

Salamah, N., Ahda, M., Bimantara, S. and Hanar, R. (2018). Total phenolic content and in vitro evaluation of antioxidant activity of ethanol extract of Ganoderma amboinense. National Journal of Physiology, Pharmacy and Pharmacology, 8:97-101.

Sharapin, N., (2000). Fundamentos de tecnología de productos fitoterapéuticos. CAB.

Singh, R., Kaur, N., Shri, R., Singh, A. P. and Dhingra, G. S. (2020). Proximate composition and element contents of selected species of Ganoderma with reference to dietary intakes. Environmental Monitoring and Assessment, 192:1-15.

Soccol, C. R., Bissoqui, L. Y., Rodrigues, C., Rubel, R., Sella, S. R., Leifa, F. and Soccol, V. T. (2016). Pharmacological properties of biocompounds from spores of the lingzhi or reishi medicinal mushroom Ganoderma lucidum (Agaricomycetes): a review. International Journal of Medicinal Mushrooms, 18:757-767.

Upadhya, V., Pai, S. R. and Hegde, H. V. (2015). Effect of method and time of extraction on total phenolic content in comparison with antioxidant activities in different parts of Achyranthes aspera. Journal of King Saud University Science, 27:204-208.

Veljović, S., Veljović, M., Nikićević, N., Despotović, S., Radulović, S., Nikšić, M. and Filipović, L. (2017). Chemical composition, antiproliferative and antioxidant activity of differently processed Ganoderma lucidum ethanol extracts. Journal of Food Science and Technology, 54:1312-1320.

Yalcin, O. U., Sarikurkcu, C., Cengiz, M., Gungor, H. and Ćavar Zeljković, S. (2020). Ganoderma carnosum and Ganoderma pfeifferi: Metal concentration, phenolic content, and biological activity. Mycologia, 112:1-8.

Zhao, C. N., Zhang, J. J., Li, Y., Meng, X. and Li, H. B. (2018). Microwave-assisted extraction of phenolic compounds from Melastoma sanguineum fruit: Optimization and identification. Molecules, 23:2498-2508.