Isolation and identification of Aspergillus species producing Ochratoxin A in Arabica coffee beans

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Published: Jul 15, 2015
Keywords:
Ochratoxin A (OTA) Arabica coffee beans Fluorescence Aspergillus group HPLC

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Kuntawee, S.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand
Akarapisan, A.
Department of Entomology and Plant Pathology, Faculty of Agriculture, Chiang Mai University, Chiang Mai 50200, Thailand

Abstract

Isolation of fungi in Arabica parchment coffee and green coffee were collected from in Omkoi district, Chiang Mai, Thailand. The coffee beans were isolated by direct plating method. The postharvest fungi of Arabica parchment coffee and green coffee were assessed for the presence of Aspergillus species, the ochratoxigenic potential of the isolates and ochratoxin A levels. Contamination by Aspergillus and Rhizopus species was found on 25.46% and 38.88% respectively, of 12 samples. Sixteen isolates were identified to species comprised Aspergillus section Ochraceus (3), Ostianus (2), Candidus (2), Sclerotiorum, Awamori, Parasiticus, Terreus and 5 isolated of Aspergillus spp. not subsequently species. The fungi group of Aspergillus were isolated to preliminary determine the Ochratoxin A (OTA) on Coconut agar (CA) incubated at 28 Co for 7 day, then observed the fluorescence under UV light with a wavelength of 365 nm. The result showed that 12 isolates of Aspergillus species produced fluorescence blue on CA medium. Confirmed by High Performance Liquid Chromatography (HPLC) found that 10 isolates produced OTA in the range of 0.004 to 12,937.50 ng/g. Aspergillus ochraceus isolates produced the maximum OTA observed in agar with toxigenic species.

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How to Cite
Kuntawee, S., & Akarapisan, A. (2015). Isolation and identification of Aspergillus species producing Ochratoxin A in Arabica coffee beans. International Journal of Agricultural Technology, 11(5), 1235–1242. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6448
Issue
Vol. 11 No. 5 (2015): July
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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