Detection of tobacco mosaic virus in petunia and tobacco cells using inclusion body staining
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Abstract
Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. The virus caused flower break, mild mosaic and brown, brittle stems in petunia; and mosaic and mottling symptoms in tobacco leaves in northern Thailand. This study evaluated virus inclusion visualization by light microscopy (VIVLM) with virus confirmation by reverse transcription polymerase chain reaction (RT- PCR).VIVLM was accomplished by staining leaf epidermal tissue with toluidine blue O and basic fuchsin and detected tobamovirus in all symptomatic samples. TMV infection was confirmed by RT-PCR; there was a 91% agreement between VIVLM and RT-PCR in tobamovirus detection. Detection of other plant virus groups by this simplified virus inclusion visualization technique may be possible.
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