Detection of tobacco mosaic virus in petunia and tobacco cells using inclusion body staining
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Abstract
Tobacco mosaic virus (TMV) causes significant losses in many economically important crops. The virus caused flower break, mild mosaic and brown, brittle stems in petunia; and mosaic and mottling symptoms in tobacco leaves in northern Thailand. This study evaluated virus inclusion visualization by light microscopy (VIVLM) with virus confirmation by reverse transcription polymerase chain reaction (RT- PCR).VIVLM was accomplished by staining leaf epidermal tissue with toluidine blue O and basic fuchsin and detected tobamovirus in all symptomatic samples. TMV infection was confirmed by RT-PCR; there was a 91% agreement between VIVLM and RT-PCR in tobamovirus detection. Detection of other plant virus groups by this simplified virus inclusion visualization technique may be possible.
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References
Christie, R. G. and Edwardson, J. R. (1994). Light and electron microscopy of plant virus inclusions. Monograph 9 (rev.) University of Florida-IFAS, Gainesville. 165 pp.
Christopher and A. B. (2001). Controlling Tobacco Mosaic Virus in Tobacco through Resistance. M.S. Dissertation. The Virginia Polytechnic Institute and State University, Virginia. 67 pp.
Jacobi, V., Bachand, G. D., Hamelin, R. C. and Castello, J. D. (1998). Development of a multiplex immunocapture RT-PCR assay for detection and differentiation of tomato and Tobacco mosaic tobamo viruses. Journal of Virology Method 74:164-178.
Kim, B. S., Ruhl, G., Creswell, T., and Loesch-Fries L. S. (2014). Molecular identification of a Tobacco mosaic virus isolate from imported petunias. Plant Health Progress 15:153.
Lesemann, D. E. (1996). Viruses recently detected in vegetatively propagated Petunia. Acta Horticulture 432:88-95.
McGovern, R.. J., Koh, L. H., To-anun, C. and Wong, S. M. (2016). Reduced incidence of tomato yellow leaf curl virus and leafminer in a tomato cultivar in northern Thailand. Crop Protection 89:273-277.
Nameth, S. (2002). Virus Diseases of Petunia. Greenhouse Product News. pp. 24-28.
Saalau, E. (2014). Tobacco Mosaic Virus Outbreak on Petunias. ISU Entomology/ Horticulture and Home Pest News. Iowa State University, Extension and Outreach. Retrieved from http://www.ipm.iastate.edu/ipm/hortnews/2014/ 04-11/tmv.html .
Scholthof, K. B. G. (2000). Tobacco mosaic virus. The Plant Health Instructor. DOI:10.1094/PHI-I-2000-1010-01 Updated 2005.
Sontirat, P, Pitakpaivan, P., Khamhangridthirong, T, Choobamroong, W, Kucprackone, U, (1994). Host Index of Plant Diseases in Thailand. 3rd ed. Mycology Section, Plant Pathology & Microbiology Division, Department of Agriculture, Bangkok, Thailand.
Walsh, K., North, J., Barker, I. and Boonham, N. (2001). Detection of different strains of Potato virus Y and their mixed infections using competitive fluorescent RT–PCR. Journal of Virological Methods 91:167-173.