Effect of plant growth regulators on in vitro morphogenesis in Gladiolus (Gladiolus hybridus Hort.) from cultured corm slice

Article Sidebar

PDF
Published: Jul 20, 2017
Keywords:
Gladiolus hybridus Corm slice Direct organogenesis Indirect organogenesis Plantlet regeneration

Main Article Content

Tripathi, M. K.
Horticultural Biotechnology Laboratory, Gwalior, MP, India
Malviya, R. K.
Horticultural Biotechnology Laboratory, Gwalior, MP, India
Vidhyashankar, M.
Department of Floriculture and Landscaping, Gwalior, MP, India
Patel, R. P.
Department of Plant Pathology, KNK-College of Horticulture, Mandsaur - 458001, RVS Agricultural University, Gwalior, MP, India

Abstract

In present research, corm slice explant of gladiolus (Gladiolus hybridus) was cultured on MS medium amended with diverse auxins and cytokinins in varying concentrations as sole as well as in different combinations. Regarding in vitro response, induction medium MS2B.5D (MS + 2.0 mgl-1 BAP + 0.5 mgl-1 2,4-D + 30.0 gl-1 sucrose + 7.5 gl-1 agar) proved well for callus initiation. MS medium supplemented with BA in range of 2.0-3.0 mgl-1  in combination with 0.5 mgl-1 NAA, 30.0 gl-1 sucrose and 7.5 gl-1 agar exhibited higher shoot proliferating efficiency, number of shoot (s) per explant and shoot of higher length. In respect to in vitro rooting response, higher root proliferating efficiency was documented on rooting medium MS.5IB.5Kn (MS + 0.5 mgl-1 IBA + 0.5 mgl-1 Kinetin + 15.0 gl-1 sucrose + 7.5 gl-1agar), while number of root (s) with higher length were recovered on rooting medium MS.5IB (MS+0.5 mgl-1 IBA+15.0 gl-1 sucrose +7.5 gl-1 agar). Phenotypically normal plantlets were acquired and subsequently transferred to pots and hardened in Environmental Growth Cabinet and Net House during initial tiring period and transferred to field successfully.

Article Details

How to Cite
Tripathi, M. K., Malviya, R. K., Vidhyashankar, M., & Patel, R. P. (2017). Effect of plant growth regulators on in vitro morphogenesis in Gladiolus (Gladiolus hybridus Hort.) from cultured corm slice. International Journal of Agricultural Technology, 13(4), 583–599. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/6765
Issue
Vol. 13 No. 4 (2017): July
Section
Original Study
Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

References

Aftab, F., Alas, M. and Afrasiab, H. (2008). In vitro shoot multiplication and callus induction in Gladiolus hybridus Hort. Pakistan Journal of Botany 40:517-522.

Ahmad, T., Ahmad, M. S., Nasir, A. and Riazuddin, S. (2000).In vitro production of cormlets in gladiolus. Pakistan Journal of Biological Sciences 3:819-821.

Aslam, F., Habib, S. and Naz, S. (2012). Effect of different phytohormones on plant regeneration of Amaryllis hippeastrum. Pakistan Journal of Science 64:54-58.

Babu, P. and Chawla, H. S. (2000). In vitro regeneration of Agrobacterium mediated transformation in gladiolus. Journal of Horticultural Science and Biotechnology 75:400-404.

Bajaj, Y. P. S., Sindhu, M. M. S. and Gill, A. P. S. (1983). Some factors affecting the in vitro propagation of gladiolus. Scientia Horticulturae 18:269-275.

Begum, S. and Haddiuzaman, S. (1995). In vitro rapid shoot proliferation and corm development in Gladiolus grandiflorus cv. Redbrand.Plant Tissue Culture 5:7-12.

Boonvanno, K. and Kanchanapoom, K. (2000). In vitro propagation of Gladiolus suranaree. Journal of Science and Technology 7:25-29.

Bose, T. K., Yadav, L. P., Pal, P., Das, P. and Parthasarathy, W. A. (2003). Commercial Flowers.Vol. II. Second edition. NayaUdyog, BidhanSarani, Kolkata.

Buch, P. O. (1972). The species. The World of Gladiolus. North American Gladiolus Council. Edgerton Press. MD. pp. 2-7.

Budiarto, K. (2009). In vitro regeneration of three gladiolus cultivars using cormel explants. Journal IlmuDasar 10:109-113.

Dantu, P. K. and Bhojwani, S. S. (1987). In vitro propagation and corm formation in gladiolus. Gartenbauwissenschaften 52:90-93.

Dantu, P. K. and Bhojwani, S. S. (1992). In vitro propagation of gladiolus: optimization of conditions for shoot multiplication. Journal of Biochemistry and Biotechnology115-118.

Dantu, P. K. and Bhojwani, S. S. (1995). In vitro corm formation and field evaluation of com - derived plants of gladiolus. Scientia Horticulturae 61:115-129.

Emek, Y. and Erdag, B. (2007). Somatic embryogenesis from leaf explants of Gladiolus anatolicus. Pakistan Journal of Biological Sciences 10:1190-1194.

Gamborg, O. L., Miller, R. A., and Ojima, K. (1968). Nutrient requirement of suspension cultures of soyabean root cells. Experimental Cell Research 50: 151-158.

Ginzburg, C. and Ziv, M. (1973).Hormonal regulation of cormel formation in Gladiolus stolons grown in vitro. Annals of Botany 37:219-224.

Goo, D. H., Joung, H. Y. and Kim, K. W. (2003). Differentiation of gladiolus plantlets from callus and subsequent flowering. Acta Horticulturae 620:339-342.

Grewel, M. S., Arora, J. S. and Gosal, S. S. (1995). Micropropagation of gladiolus through in vitro cormel production. Plant Tissue Culture 5:27-33.

Hussain, I. A., Muhammad, H. R. and Quraishi, A. (2001). In vitro multiplication of gladiolus. Plant Tissue Culture 11:121-126.

Hussain, S. C. T., Geetha, C. K., Rajeevan, P. K. and Valsalakumari, P. K. (1994). Plant regeneration from root derived callus in gladiolus. Journal of Ornamental Horticulture 2:46-50.

Hussey, G. (1977). In vitro propagation of gladiolus by precocious auxiliary shoot formation. Scientia Horticulturae 6:296.

Kamo, K. (1995). A. Cultivar comparison of plant regeneration from suspension cells, callus and cormel slices of gladiolus in vitro. Cell and Developmental Biology 31:113-115.

Kamo, K., Chen, J. and Lawson, R. (1990). The establishment of cell suspension cultures of gladiolus that regenerate plants in vitro. Cell and Developmental Biology 26:425-430.

Kamo, K. (1994). Effect of phytohormones on plant regeneration from callus of gladiolus cultivar’s Jenny Lee in vitrom Cell and Developmental Biology 30:26-31.

Kasumi, M., Takatsu, Y., Tometsune, H. and Sakuma, F. (1998). Somatic embryogenesis, organogenesis and plant regeneration from leaf of in vitro grown gladiolus. Bullet Ibaraki Plant Biotechnology 2: 83-90.

Kumar, A., Sood, A., Palni, L. M. S. and Gupta, A. K. (1999). In vitro propagation of Gladiolus hybrids Hort. synergistic effect of heat shock and sucrose on morphogenesis. Plant Cell, Tissue and Organ Culture 57:105-112.

Kumar, A., Palni, L. M. S., Sood, A., Sharm, M., Palni, U. T. and Gupta, A. K. (2002). Heat-shock induced somatic embryogenesis in callus cultures of gladiolus in the presence of high sucrose. Journal of Horticultural Science and Biotechnology 77:73-78.

Lehsem, Y. Y. (1998). Plant senescence processes and free radicals. Free Radical Biology and Medicine 5:7-12.

Lilien-Kipnis, H. and Kochba, M. (1987). Mass propagation of new gladiolus hybrids. Acta Horticulturae 212:631-638.

Longan, A. E. and Zettler, F. (1985). Rapid in vitro propagation of virus indexed gladiolus. Acta Horticulturae 164:169-80.

Memon, N. (2012). In vitro propagation of gladiolus plantlets and cormels. Journal of Horticultural Science and Ornamentals Plants 4:280-291.

Memon, N., Qasim, M., Jaskani, M. J. and Ahmad, R. (2010). In vitro cormel production of gladiolus. Pakistan Journal of Botany 47:115-123.

Misra, S. and Singh, R. (1999). In vitro propagation of gladiolus cv. ‘American Beauty’ J Journal of Ornamental Horticulture 18:67-70.

Murashige, T.and Skoog, F. (1962).A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia Plantarum 15: 473-497.

Nagaraju, V. and Parthasarathy, V. A. (1995). Effect of growth regulators on in vitro shoots of Gladiolus hybrids. Folia Horticulturae 7:93-100.

Pathania, N. S., Misra, R. L. and Raghava, S. P. S. (2001). Precocious shoot proliferation and micro corm production in gladiolus through culture. Journal of Ornamental Horticulture 20:69-73.

Prasad, V. S. S.and Gupta, S. D. ( 2006). In vitro shoot regeneration of gladiolus in semi-solid agar versus liquid cultures with support systems. Plant Cell, Tissue and Organ Culture 87:263-271.

Priyakumari, I. and Sheela, V. L. (2005).Micropropagation of gladiolus cv. ‘Peach blossom’ through enhanced release of auxiliary buds. Journal of Tropical Agriculture 43:47-50.

Remotti, P. C. (1995). Callus induction and plant regeneration from gladiolus. Plant Science 170:205-214.

Roy, S. K., Gangopadhyay, G., Bandyopadhyay, T., Modak, B. K., Datta, S. and Mukhrajee, K. K. (2006). Enhancement of in vitro micro-corm production in gladiolus using alternative matrix. African Journal of Biotechnology 5:1204-1209.

Sen, J. and Sen, S. (1995). Two step bud culture technique for a high regeneration of gladiolus corm. Scientia Horticulturae 64:133-138.

Simonsen, J. and Hindebrandt, A. C. (1971). In vitro growth and differentiation of gladiolus plants from tissue culture. Canadian Journal of Botany 49:1817-1819.

Sinha, P. and Roy, S. K. (2002).Plant regeneration through in vitrocormel formation from callus culture of Gladiolus primulinus Baker. Plant Tissue Culture 12:139-145.

Steel, R. G. D., Torri, J. H. and Dickey, D. A. (1997). Principles and procedures of statistics.A Biometric approach. Third edition. McGraw Hill Book Co. Inc. New York, USA.

Stefaniak, B. (1994). Somatic embryogenesis and plant regeneration of gladiolus. Plant Cell Reports 13:386-389.

Steinitz, B, Cohen, A, Goldberg, Z, Kochba, M. (1991). Precocious gladiolus corm formation in liquid shake culture. Plant Cell, Tissue and Organ Culture 26:63-70.

Steinitz, B. and Lilien-Kipnis, H. (1989).Control of precocious gladiolus corm and cormel formation in tissue culture. Journal of Plant Physiology 135:495-500.

White, P. R. (1963). The cultivation of animal and plant cell. Ronald Press Co, New York. 239 pp.

Ziv, M. (1979). Transplanting gladiolus plants propagated in vitro. Horticulture 11:257-260.

Ziv, M. and Lilien-Kipnis, H. (2000). Bud regeneration form inflorescence explants for rapid propagation of geophytes in vitro. Plant cell Reports 19:845-850.