Effect of Bacillus sp. AAHMRU15 antagonist in combination with yeast extract product (Beta-Sac Plus®) on the inhibition of AHPND−causing Vibrio parahaemolyticus (VpAHPND)
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Abstract
Acute hepatopancreatic necrosis disease (AHPND) has caused huge losses in Pacific white shrimp (Litopenaeus vannamei) culture in Thailand since 2013. The causative agent of AHPND was originally reported to be a specific strain of Vibrio parahaemolyticus (VpAHPND). In this study, the in vitro antagonistic effect of Bacillus sp. AAHMRU15 against 4 strains of VpAHPND (VP01, VP02, VP03, and VP04) was clearly demonstrated. The effect of the yeast extract product named Beta-Sac Plus® (BS) on the inhibitory activity of AAHMRU15 was determined through agar well diffusion assay. The results suggested that BS increased the antibacterial activity. The real-time growth of VpAHPND cultured in the cell-free media containing extracellular products of AAHMRU15 alone (CFS) or in combination with Beta-Sac Plus® (CFS+BS) was monitored. The results indicated that the antagonistic Bacillus sp. AAHMRU15 and Beta-Sac Plus® were able to inhibit the growth of VpAHPND. The obtained data introduced the potential tool to control the pathogenic bacteria in white shrimp culture through biologically, environmentally friendly and safety practice. Our findings provide an alternative way to reduce the use of antibiotics in support to the concept of susceptible aquaculture and safety food.
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