Exploring the therapeutic efficacy of ethanol extracts from Melia azedarach Linn. fruit-seeds against oxidative stress induced by melon flies on bitter melon.
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Abstract
The application of ethanol extract of fruit-seeds Melia azedarach (FSMA) resulted in a significant, concentration-dependent reduction in malondialdehyde (MDA) and hydrogen peroxide (H2O2) levels (p < 0.05), indicating a marked attenuation of oxidative stress. Enzymatic assays demonstrated that FSMA treatments, particularly at 125 and 652 ppm, significantly enhanced the activities of key antioxidant enzymes, including superoxide dismutase (SOD), catalase (CAT), and peroxidase (POD). A strong inverse correlation was observed between the levels of oxidative stress biomarkers and the activities of these antioxidative enzymes, suggesting a mechanistic role of FSMA in modulating redox homeostasis. The findings underscore the efficacy of FSMA in mitigating oxidative damage through the activation of endogenous antioxidant defense systems. These results support the potential use of FSMA as a sustainable and natural alternative to synthetic pesticides, contributing to the development of eco-friendly strategies for oxidative stress management in agricultural systems.
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References
Ahmed, M. F., Ahmed, M. A., Thayyil, H., Zameeruddin, K. and Ibrahim, M. (2008). Antioxidative activity of Melia azedarach Linn leaf extract. Iranian Journal of Pharmacology & Therapeutics, 7:31-34.
Aljohi, A., Matou-Nasri, S. and Ahmed, N. (2016). Antiglycation and antioxidant properties of Momordica charantia. PLoS ONE, 11:0159985.
Azhar, F., Latif, A., Rafay, M. Z., Iqbal, A., Anwar, I., Waheed, Z. and Mushtaq, R. M. Z. (2022). Preliminary studies and in-vitro antioxidant activity of fruit-seed extracts of Melia azedarach Linn. International Journal of Innovative Science and Research Technology, 7:1328-1335.
Chaki, M., Begara-Morales, J. C. and Barroso, J. B. (2020). Oxidative stress in plants. Antioxidants, 9:481.
Choudhary, A., Kumar, A. and Kaur, N. (2020). ROS and oxidative burst: Roots in plant development. Plant Diversity, 42:33-43.
Dandana, A., Gammoudi, I., Ferchichi, S., Chahed, H., Limam, H. B., Addad, F. and Miled, A. (2011). Correlation of oxidative stress parameters and inflammatory markers in tunisian coronary artery disease patients. International Journal of Biomedical Science, 7:6-13.
Dhillon, M. K., Naresh, J. S., Singh, R. and Sharma, N. K. (2005). Reaction of different bitter melon (Momordica charantia L.) genotypes to melon fly melon, Bactrocera cucurbitae (Coquillett). Indian Journal of Plant Protection, 33:55-59.
Durak, R., Dampc, J., Kula-Maximenko, M., Mołoń, M. and Durak, T. (2021). Changes in antioxidative, oxidoreductive and detoxification enzymes during development of aphids and temperature increase. Antioxidants, 10:1181.
Haddidi, I., Duc, N. H., Tonk, S., Rápó, E. and Posta, K. (2020). Defense enzymes in mycorrhizal tomato plants exposed to combined drought and heat stresses. Agronomy, 10:1657.
Jan, R., Asaf, S., Numan, M., Lubna. and Kim, K. M. (2021). Plant secondary metabolite biosynthesisand transcriptional regulation inresponse to biotic and abiotic stress conditions. Agronomy, 11:968.
Kar, D. R., Ghosh, P., Suresh, P., Chandra, S. and Paul, D. (2022). Review on phyto-chemistry and pharmacological activity of Melia azedarach. International Journal of Experimental Research and Review, 28:38-46.
Kaur, A., Sohal, S. K., Arora, S., Kaur, H. and Kaur, A. P. (2014). Effect of plant extracts on biochemistry of Bactrocera cucurbitae (Coquillett). Journal of Entomology and Zoology Studies, 2:86-92.
Kerchev, P. I. and Breusegem, F. V. (2022). Improving oxidative stress resilience in plants. The Plant Journal, 109:359-372.
Lei, X. G., Zhu, J. H., Cheng, W. H., Bao, Y, Ho, Y. S., Reddi, A. R., Holmgren, A. and Arnér, E. S. J. (2016). Paradoxical roles of antioxidant enzymes: Basic mechanisms and health implications. Physiological Reviews, 96:307-364.
Liu, X., Fu, Z. X., Kang, Z. W., Li, H., Liu, T. X. and Wang, D. (2022). Identification and characterization of antioxidant enzyme genes in parasitoid Aphelinus asychis (Hymenoptera: Aphelinidae) and expression profiling analysis under temperature stress. Insects, 13:447.
Nhung, T. T. P. and Quoc, L. P. T. (2023). Acute and sub-chronic toxicity studies of the ethanol extract of Erythrina fusca Lour. fruit via oral administration in mice. Acta Biologica Szegediensis, 67:111-122.
Nhung, T. T. P. and Quoc, L. P. T. (2024). Evaluating the insecticidal potential of ethanol extracts from Melia azedarach Linn. against Bactrocera cucurbitae - a pest inflicting damage on Momordica charantia Linn. Journal of Plant Biotechnology, 51:89-99.
Olufunmilayo, E. O., Gerke-Duncan, M. B. and Holsinger, R. M. D. (2023). Oxidative stress and antioxidants in neurodegenerative disorders. Antioxidants, 12:517-547.
Raina, K., Kumar, D. and Agarwal, R. (2016). Promise of bitter melon (Momordica charantia) bioactives in cancer prevention and therapy. Seminars in Cancer Biology, 40–41:116-129.
Saleem, S., Mushtaq, N. U., Shah, W. H., Rasool, A., Hakeem, K. R. and Rehman, R.U. (2022). Beneficial role of phytochemicalsin oxidative stress mitigation in plants. In: T. Aftab and K. R. Hakeem (Eds.), Antioxidant Defense in Plants. Molecular Basis of Regulation, Singapore, Springer, pp. 435-451.
Shohag, S., Akhter, S., Islam, S., Sarker, T., Sifat, M. K., Rahman, M. M., Islam, M. R. and Sharma, R. (2022). Perspectives on the molecular mediators of oxidative stress and antioxidant strategies in the context of neuroprotection and neurolongevity: An extensive review. Oxidative Medicine and Cellular Longevity, 2022:7743705.
Somegowda, M., Raghavendra, S., Sridhara, S., Rajeshwara, A. N., Pramod, S. N., Shivashankar, S., Lin, F., El-Abedin, T. K. Z., Wani, S. H. and Elansary, H. O. (2021). Defensive mechanisms in cucurbits against melon fly (Bactrocera cucurbitae) infestation through excessive production of defensive enzymes and antioxidants. Molecules, 26:6345.
Sule, R. O., Condon, L. and Gomes, A. V. (2022). A common feature of pesticides: Oxidative stress - The role of oxidative stress in pesticide-induced toxicity. Oxidative Medicine and Cellular Longevity, 2022:5563759.
Touzout, S. N., Merghni, A., Laouani, A., Boukhibar, H., Alenazy, R., Alobaid, A., Alenazy, M., Ben-Attia, M., Saguem, K. and El-Bok, S. (2023). Antibacterial Properties of methanolic leaf extracts of Melia azedarach L. against gram-positive and gram-negative pathogenic bacteria. Microorganisms, 11:2062.
Yeshi, K., Crayn, D., Ritmejerytė, E. and Wangchuk, P. (2022). Plant secondary metabolites produced in response to abiotic stresses has potential application in pharmaceutical product development. Molecules, 27:313.