In vitro effect of gamma irradiation and plant growth regulators (PGRs) for induction and development of Stylosanthes hamata cv. Verano
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Abstract
Pasture legumes was developed by tissue culture technique using gamma irradiation. Seeds of Stylosanthes hamata cv. Verano were received 7 doses of radiation (5, 10, 15, 20, 25, 30 and 45 Krad) and control (without irradiation) cultured on MS medium. Free Plant Growth Regulators (PGRs) and 30 g/L sucrose treatment found that 30 Krad decreased the percentage of shoot and root length of 31.7% and 20.51% respectively in 30 days. While seeds without irradiation were 100% germination. The radiation doses was reduced the growth rate by half in 7 days GR50(30) at 39.82 Krad. The lethal dose (LD50) of Stylosanthes hamata cv. Verano was 26.14 Krad and the higher irradiation level increased death rate. Seeds on MS medium with 3 mg/L of meta-Topolin (mT) resulted at 60 days after irradiation which higher than 50 % in all treatments. Moreover, every dose of gamma rays stimulated shoots and roots after 60 days.
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