Effects of light intensity on the growth, photosynthesis and leaf microstructure of hydroponic cultivated spinach (Spinacia oleracea L.) under a combination of red and blue LEDs in house
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Abstract
The effect of four different light intensities (90, 140, 190 and 240 µmol/m2/s) on the growth, photosynthesis and leaf microstructure of hydroponic cultivated spinach under a combination of red and blue LEDs (R660/B450 = 80/20) in house was investigated. The plant height, leaf number, leaf area, NGR, NAR, Chla, Chl(a +b), photosynthetic capacity increased with increasing intensity. Althrough, there was not statistically significant difference in Chl(a+b) and carotenoid contents between the 190 and 240 µmol/m2/s treatments but they differed in Chla. Furthermore, the leaf area, NGR, NAR, Chla and Chl(a+b) contents were significantly higher in 190 µmol/m2/s treatment than 240 µmol/m2/s treatment. Differences in leaf thickness, palisade tissue length and spongy tissue length were statistically significant between 4 treatments. Even, leaf thickness in 190 µmol/m2/s treatment was found by 1.4 folds increased compare with 90 µmol/m2/s treatment. When light intensity increased, epidermal cell area, stomatal length, stomatal width increased. In the adaxial leaf surface and abaxial leaf surface, the epidermal cell area was highest in 190 µmol/m2/s treatment. But in all two leaf surfaces stomatal width was highest in the 240 µmol/m2/s treatment. The results showed that fresh weight and dry weight of stem and leaf, theoretical yeild, final harvest yeild were not highest in 240 µmol/m2/s treatment but in 190 µmol/m2/s treatment. Our results suggested that 190 µmol/m2/s light intensity may be appropriated the intensity for growth of spinach.
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References
Arnon, D. I. (1949). Copper enzymes in isolated chloroplasts, polyphenoloxidase in Beta vulgaris. Plant Physiology. 24:1-15.
Boese, S. R. and Huner, N. P. (1990). Effect of growth temperature and temperature shifts on spinach leaf morphology and photosynthesis. Plant Physiology. 94:1830-1836.
Chen, X. L., Guo, W. Z., Xue, X. Z., Wang, L. C. and Qiao, X. J. (2014). Growth and quality responses of ‘Green Oak Leaf’lettuce as affected by monochromic or mixed radiation provided by fluorescent lamp (FL) and light-emitting diode (LED). Scientia Horticulturae. 172:168-175.
Clark, G. (1981). Staining Procedures. 4th edn. London: Williams and Wilkins. 325-326.
Dong, C., Fu, Y., Liu, G. and Liu, H. (2014). Growth, photosynthetic characteristics, antioxidant capacity and biomass yield and quality of wheat (Triticum aestivum L.) exposed to LED light sources with different spectra combinations. Journal of agronomy and crop science. 200:219-230.
Fan, X. X., Xu, Z. G., Liu, X. Y., Tang, C. M., Wang, L. W. and Han, X. (2013). Effects of light intensity on the growth and leaf development of young tomato plants grown under a combination of red and blue light. Scientia Horticulturae. 153:50-55.
Goins, G. D., Yorio, N. C., Sanwo, M. M. and Brown, C. S. (1997). Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. Journal of experimental botany. 48:1407-1413.
Hoffmann, W. A. and Poorter, H. (2002). Avoiding bias in calculations of relative growth rate. Annals of botany. 90:37-42.
Johkan, M., Shoji, K., Goto, F., Hahida, S. N. and Yoshihara, T. (2012). Effect of green light wavelength and intensity on photomorphogenesis and photosynthesis in Lactuca sativa. Environmental and Experimental Botany. 75:128-133.
Kim, S. J., Hahn, E. J., Heo, J. W. and Paek, K. Y. (2004). Effects of LEDs on net photosynthetic rate, growth and leaf stomata of chrysanthemum plantlets in vitro. Scientia Horticulturae. 101:143-151.
Lefsrud, M. G., Kopsell, D. A. and Sams, C. E. (2008). Irradiance from distinct wavelength light-emitting diodes affect secondary metabolites in kale. HortScience. 43:2243-2244.
Li, Q. and Kubota, C. (2009). Effects of supplemental light quality on growth and phytochemicals of baby leaf lettuce. Environmental and Experimental Botany. 67:59-64.
Li, H. M., Lu, X. M. and Gao, Q. H. (2016). Effects of different light qualities on the growth, photosynthetic pigments and stomatal characteristics of okra (Abelmoschus esculentus) seedlings. Acta Pratac Sin. 25:26-70.
Lian, M. L., Murthy, H. N. and Paek, K. Y. (2002). Effects of light emitting diodes (LEDs) on the in vitro induction and growth of bulblets of Lilium oriental hybrid ‘Pesaro’. Scientia Horticulturae. 94:365-370.
XiaoYing, L., ShiRong, G., ZhiGang, X., XueLei, J. and Tezuka, T. (2011). Regulation of chloroplast ultrastructure, cross-section anatomy of leaves, and morphology of stomata of cherry tomato by different light irradiations of light-emitting diodes. HortScience. 46:217-221.
Macedo, A. F., Leal-Costa, M.V.T, Eliana, S. L., Celso, L. S., Esquibel, M. A. (2011). The effect of light quality on leaf production and development of in vitro-cultured plants of Alternanthera brasiliana Kuntze. Environmental and experimental botany. 70:43-50.
Matos, F. S., Wolfgramm, R., Gonçalves, F. V., Cavatte, P. C., Ventrella, M. C. and DaMatta, F. M. (2009). Phenotypic plasticity in response to light in the coffee tree. Environmental and experimental botany. 67:421-427.
Nhut, D. T., Takamura, T., Watanabe, H., Okamoto, K. and Tanaka, M. (2003). Responses of strawberry plantlets cultured in vitro under superbright red and blue light-emitting diodes (LEDs). Plant Cell, Tissue and Organ Culture. 73:43-52.
Pereira, W. E., de Siqueira, D. L., Martínez, C. A. and Puiatti, M. (2000). Gas exchange and chlorophyll fluorescence in four citrus rootstocks under aluminium stress. Journal of plant physiology. 157:513-520.
Qin, Y. Z., Xing, Z., Zou, J. F., He, C. Z., Li, Y. L. and Xiong, X. Y. (2014). Effects of sustained weak light on seedling growth and photosynthetic characteristics of potato seedlings. Scientia Agricultura Sinica. 47:537-545.
Radford, P. J. (1967). Growth Analysis Formulae-Their Use and Abuse 1. Crop science. 7:171-175.
Sabzalian, M. R., Heydarizadeh, P., Zahedi, M., Boroomand, A., Agharokh, M., Sahba, M. R. and Schoefs, B. (2014). High performance of vegetables, flowers, and medicinal plants in a red-blue LED incubator for indoor plant production. Agronomy for sustainable development. 34:879-886.
Steinger, T., Roy, B. A. and Stanton, M. L. (2003). Evolution in stressful environments II: adaptive value and costs of plasticity in response to low light in Sinapis arvensis. Journal of evolutionary biology. 16:313-323.
Taiz, L. and Zeiger, E. (2003). Plant physiology. 3rd edn. Annals of Botany. 91:750-751.
Terashima, I., Fujita, T., Inoue, T., Chow, W. S. and Oguchi, R. (2009). Green light drives leaf photosynthesis more efficiently than red light in strong white light: revisiting the enigmatic question of why leaves are green. Plant and cell physiology. 50:684-697.
Tran, T. A., Vassileva, V., Petrov, P. and Popova, L. P. (2013). Cadmium-induced structural disturbances in Pisum sativum leaves are alleviated by nitric oxide. Turkish Journal of Botany. 37:698-707.
Van Kooten, O. and Snel, J. F. (1990). The use of chlorophyll fluorescence nomenclature in plant stress physiology. Photosynthesis research. 25:147-150.
Vogelmann, T. C. and Han, T. (2000). Measurement of gradients of absorbed light in spinach leaves from chlorophyll fluorescence profiles. Plant, Cell and Environment. 23:1303-1311.
Wheeler, R. M., Mackowiak, C. L. and Sager, J. C. (1991). Soybean stem growth under high-pressure sodium with supplemental blue lighting. Agronomy Journal. 83:903-906.
Yao, X. Y., Liu, X. Y., Xu, Z. G. and Jiao, X. L. (2017). Effects of light intensity on leaf microstructure and growth of rape seedlings cultivated under a combination of red and blue LEDs. Journal of Integrative Agriculture. 16:97-105.
Zhang, S., Ma, K. and Chen, L. (2003). Response of photosynthetic plasticity of Paeonia suffruticosa to changed light environments. Environmental and experimental botany. 49:121-133.
