The growth of Antrodia cinnamomea mycelia on different kinds of substrates in Thailand
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Abstract
The cultivation of Antrodia cinnamomea on the different kinds of substrates was investigated. Firstly, it was cultivated on sawdusts from five sources: Dolichandrone serrulata, Samanea saman, Mangifera indica, Hevea brasiliensis and Cinnamomum verum. It was found that the mushroom mycelia grew well on mango sawdust the average initial growth time was 9.40 days and the average time the mycelia fully colonized the sawdust surface was 90.80 days. The mycelia had abundant density and orange color at 70 days, with cinnamon odor at 110 days. Secondly, it was cultivated on the Hordeum vulgare, Sorghum sp., two varieties of rices, Sesamum indicum, Coix sp. and Vigna radiata. It was found that the mycelia grew well on millet grass with initial growth time in 9.86 days and it fully colonized the substrate in 26.14 days. The mycelia had abundant density and orange color in 50 days, with cinnamon odor in 110 days. Finally, the mycelia were cultivated on five liquid media: PD, PMD, PPD, PYD and PMPYD. It revealed that the mycelia grew well on PMPYD. It had abundant density with yellow-orange colors when it was 90 days. The mycelia fully covered the surface of the media in 60.00 days. Its colony was a thick mat and floated on the surface of the liquid media. The average dry weight of the mycelia was 0.528 g/100 ml in 90 days and the pH value of liquid media was increased during the growth of the mycelia. In addition, when the quantities of the bioactive compounds, adenosine and cordycepin, from dried mycelia which cultivated on the Mango sawdust, Millet grass seeds and the PMPYD were analyzed by the HPLC, adenosine was found at 202.23, 25.25 and 81.90 mg/100 g dried weight mycelia, respectively. However, 499.69 mg/100 g of cordycepin was found only in mycelia cultivated on Mango sawdust. It revealed that the mycelia had different quantity nutrients such as protein, fat, carbohydrate, ash and moisture.
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