Production of exopolysaccharide from Tricholoma crassum in submerged culture and its antioxidant activities
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Abstract
The effects of carbon, nitrogen sources and nutritional requirements were studied for the production of exopolysaccharide (EPS) from Tricholoma crassum using the statistically based experimental design in a shake flask culture, and the antioxidant activities of EPS in vitro. Glucose and Yeast extract was found to be the most suitable of carbon and nitrogen source. The optimization of submerged culture conditions was studied using Taguchi method; the results showed the optimized cultivation was pH 6.5, incubation temperature 30°C, cultivation time 10 days. Under these optimal conditions, the EPS content and dry cell weight were 0.727 g/L and 9.7 g/L, respectively. The EPS consisted of galactose, glucose and mannose with ratio 1:6.25:17.72, suggested that EPS from T. crassum could be Galacto-Gluco-Mannan. The EPS demonstrated positively antioxidant potential on 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging, reducing power and inhibition of lipid peroxidation assay.
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References
Banerjee, D., Jana, M. and Mahapatra, S. (2009). Production of exopolysaccharide by endophytic Stemphylium sp. Mycologia Aplicada International. 21:57-62.
Chang, M., Tsai, G. and Houng, J. (2005). Optimization of the medium composition for the submerged culture of Ganoderma lucidum by Taguchi array design and steepest ascent method. Enzyme and Microbial Technology. 38:407-414.
Chang, S. T. and Hayes, W. A. (1978). The Biology and Cultivation of Edible Mushrooms; Academic Press: New York.
Cho, E. J., Oh, J. Y., Chang, H. Y. and Yun, J. W. (2006). Production of exopolysaccharide by submerged mycelia culture of a mushroom Tremella fuciformis. Journal of Biotechnology. 127:129-140.
Dubois, M., Gilles, K. A., Hamilton, J. K., Rebers, P. A. and Smith, F. (1956). Colorimetric method for determination of sugar and related substances. Analytical Chemistry. 28:350-356.
El-Dein, M. M. N., El-Fallal, A. A., Toson, E. A. and Hereher, F. A. (2004). Exopolysaccharide production by Pleurotus pulmonarius: Factors affecting formation and their structures. Pakistan Journal of Biological Science. 7:1078-1084.
Elisashvili, V. I., Kachlishvili, E. T. and Wasser, S. P. (2009). Carbon and nitrogen source effects on basidiomycetes exopolysaccharide production. Applied Biochemistry and Microbiology. 45:592-596.
Frankel, E. N. and Meyer, A. S. (2000). The problems of using one dimensional method to evaluate multifunctional food and biological antioxidants. Journal of the Science of Food and Agriculture. 80:1925-1941.
Gao, C., Wang, Z., Su, T., Zhang, J. and Yang, X. (2012). Optimization of Exopolysaccharide production by Gomphidius rutilus and its antioxidant activities in vitro. Carbohydrate Polymers. 87:2299-2305.
Huang, H. C. and Liu, Y. C. (2008). Enhancement of polysaccharide production by optimization of culture conditions in shake fask submerged cultivation of Grifola umbellate. Journal of Chinese Institute of Chemical Engineers. 39:307-311.
Kim, S. W., Xu, C. P., Hwang, H. J., Choi, J. W., Kim, C. W. and Yun, J. W. (2003). Production and Characterization of Exopolysaccharides from an Enthomopathogenic Fungus Cordycepsmilitaris NG3. Biotechnology Progress. 19:428-435.
Kim, H. O., Lim, J. M., Joo, J. H., Kim, S. W., Hwang, H. J., Choi, J. W. and Yun, J. W. (2005). Optimization of submerged culture condition for the production of mycelial biomass and exopolysaccharides by Agrocybe cylindracea. Bioresource Technology. 96:1175-1182.
Kim, S. S., Lee, J., Cho, J., Kim, Y. E. and Hong, E. K. (2010a). Effects of C/N ratio and Trace Elements on Mycelial Growth and Exo-polysaccharide Production of Tricholoma matsutake. Biotechnology and Bioprocess Engineering. 15:293-298.
Kim, S. S., Lee, J., Cho, J., Kim, Y. E. and Hong, E. K. (2010b). Process development for mycelia growth and polysaccharide production in Tricholoma matsutake liquid culture. Journal of Bioscience and Bioengineering. 109:351-355.
Kohen, R. and Nyska. A. (2002). Oxidation of biological systems: oxidative stress phenomena, antioxidants, redox reactions, and method for their quantification. Toxicologic Pathology. 30:620-650.
Kozarski, M., Klaus, A., Niksic, M., Jakovljevic, D., Helsper, J. P. F. G. and Griensven, L. J. L. V. (2011). Antioxidative and immunomodulating activities of polysaccharide extracts of the mecicinal mushrooms Agaricus bisporus, Agaricus brailiensis, Ganoderma lucidum and Phellinus linteus. Food Chemistry. 129:1667-1675.
Lai, W. H., Salleh, S. M, Daud, F., Zainal, Z., Othman, A. M., and Saleh, N. M. (2014). Optimization of submerged culture conditions for the production of mycelial biomass and exopolysaccharides from Lignosus rhinoceros. Sains Malaysiana. 43:73-80.
Le. J., Hu, S. and Xu, M. (2007). Optimisation of submerged culture conditions for the production of mycelia biomass and exopolysaccharide by Pleurotus nebrodensis. Annals of Microbiology. 57:389-393.
Lee, B. C., Bae, J. T., Pyo, H. B., Choe, T. B., Kim, S. W., Hwang, H. J. and Yun, J. W. (2004). Submerged culture conditions for the production of mycelia biomass and exopolysaccharide by edible Basidiomycete Grifola frondosa. Enzyme Microbial Technology. 35:369-376.
Lin, R., Liu, H., Wu, S., Pang, L., Jia, M., Fan, K., Jia, S. and Jia, L. (2012). Production and in vitro antioxidant activity of Exopolysaccharide by a mutant, Cordyceps militaris SU5-08. International Journal Biological Macromolecules. 51:153-157.
Meng, F., Zhou, B., Lin, R., Jia, L., Liu, X., Deng, P., Fan, K., Wang, G., Wang, L. and Zhang, J. (2010). Extraction optimization and in vivo antioxidant activities of Exopolysaccharide by Morchella esculenta SO-01. Bioresource Technology. 101:4564-4569.
Nehad, E. A. and El-Shamy, A. R. (2010). Physiological studies on the production of exopolysaccharide by Fungi. Agriculture and Biology Journal of North America. 1:1303-1308.
Patra, P., Bhanja, S. K., Sen, I. K., Nandi, A. K., Samanta, S., Das, D., Devi, S. P. K., Maiti, T. K., Acharya, K. and Islam, S. S. (2012). Structural and immunological studies of hetero polysaccharide isolated from the alkaline extract of Tricholoma crassum (Berk.) Sacc. Carbohydrate Research. 362:1-7.
Ray, S., Sarkar, S. and Kundu, S. (2011). Extracellular biosynthesis of silver nano particals using the mycorrhizal mushroom Tricholoma crassum (Berk.) Sacc.: Its antimicrobial activity against pathogenic bacteria ans fungus, including multidruf resistant plant and human bacteria. Digest Journal of Nanomaterials and Biostructures. 6:1289-1299.
Roukas, T. and Biliaderis, C. G. (1995). Evaluation of carob pod as a substrate for pullulan production by Aureobasidium pullulans. Apply Biochemistry and Biotechnology. 55:27-43.
Samanta, S., Maity, K., Nandi, A. K., Sen, I. K., Devi, K. S. P., Mukherjee, S., Maiti, T. K., Acharya, K. and Islam, S. S. (2013). A glucan from an ectomycorrhizal edible mushroom Tricholoma crassum (Berk.) Sacc.: isolation, characterization, and biological studies. Carbohydrate Research. 367:33-40.
Shon, M. Y., Kim, T. H. and Sung, N. J. (2003). Antioxidants and free radical scavenging activity of Phellinus baumii (Phellinus of Hymenochaetaceae) extracts. Food Chemistry. 82:593-597.
Subhadip, S. and Banerjee, D. (2013). Evaluation of in vitro antioxidant potency of exopolysaccharide from endophytic Fusarium solani SD5. International Journal of Biological Macromolecules. 53:62-66.
Subrata, G., Biswas, G., Pradhan, P., Mandal, S. C. and Acharya, K. (2012). Antimicrobial activities of basidiocarps of wild edible mushrooms of West Bengal, India. International Journal of PharmTech Research. 4:1554-1560.
Sun, H., Mao, W., Jiao, J., Xu, J., Li, H., Chen, Y., Qi, X., Chen, Y., Xu, J., Zhao, C., Hou, Y. and Yang, Y. (2011). Structural characterization of extracellular polysaccharides produced by the marine fungus Epicoccum nigrum JJY-40 and their antioxidant activities. Marine Biotechnology. 13:1048-1055.
Teaumroong, N., Sattayapisut, W., Teekachunhatean, T. and Boonkerd, N. (2002). Using agricultural wastes for Tricholoma crassum (Berk.) Sacc. production. In Microbiology of Composting; Insam, H., Riddech, N., Klammer, S., Eds.; Springer: Berlin, Heidelberg. pp. 231-236.
Wu, X., Xu, R., Ren, Q., Bai, J. and Zhao, J. (2012). Factors affecting extracellular and intracellular polysaccharide production in submerged cultivation of Tricholoma mongolicum. African Journal of Microbiology Research. 6:909-916.
Yan, J. K., Li, L., Wang, Z. M., Leung, P. H., Wang, W. Q. and Wu, J. Y. (2009). Acidic degradation and enhanced antioxidant activities of Exopolysaccharides from Cordyceps sinensis mycelia culture. Food Chemistry. 177:641-646.
