Enhancing Columnea latent viroid detection using reverse transcription loop-mediated isothermal amplification (RT-LAMP)

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Published: Mar 15, 2019
Keywords:
Detection RT-LAMP viroids leaves seeds

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Bhuvitarkorn, S.
Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand,Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand
Klinkong, S.
Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand
Reanwarakorn, K.
Department of Plant Pathology, Faculty of Agriculture at Kamphaeng Saen, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand, Center for Advanced Studies for Agriculture and Food, KU Institute for Advanced Studies, Kasetsart University, Bangkok 10900, Thailand (CASAF, NRU-KU, Thailand), Center for Agricultural Biotechnology, Kasetsart University, Kamphaeng Saen Campus, Nakhon Pathom 73140, Thailand, Center of Excellence on Agricultural Biotechnology: (AG-BIO/PERDO-CHE), Bangkok 10900, Thailand.

Abstract

Columnea latent viroid (CLVd) is an important quarantine plant pathogen, it is of regulatory interest because of its high rate of seed transmission in tomato (Solanum lycopersicum). The detection of CLVd from leaves and seeds of solanaceous and cucurbitaceae species were investigated. A reverse transcription loop-mediated isothermal amplification (RT-LAMP) method was developed for detection of CLVd within 1 h. The method is highly sensitive, being 2,000 times and 100 times more sensitive than conventional RT-PCR in detecting CLVd from leaves and seeds, respectively. The results showed that this detection method was species specific to CLVd with no cross reactivity to other viroids in the same Pospiviroid genus. It was successfully applied to detect CLVd from field samples. This appears to be the first application of RT-LAMP for detecting CLVd.

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How to Cite
Bhuvitarkorn, S., Klinkong, S., & Reanwarakorn, K. (2019). Enhancing Columnea latent viroid detection using reverse transcription loop-mediated isothermal amplification (RT-LAMP). International Journal of Agricultural Technology, 15(2), 215–228. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/8180
Issue
Vol. 15 No. 2 (2019): March
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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