Age-related difference changes semen quality and seminal plasma protein patterns of Thai native rooster

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Published: Mar 15, 2019
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seminal plasma proteins semen quality Thai native rooster

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Inyawilert, W.
Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand, The center for Agricultural Biotechnology, Naresuan University, Phitsanulok, 65000, Thailand
Rungruangsak, J.
Bureau of Biotechnology in Livestock Production, Pathum Thani, 12000, Thailand
Chanthi, S.
Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand
Liao, Y. J.
4 Department of Animal Science, National Chung Hsing University, Taichung, 40227, Taiwan, Division of Physiology, Livestock Research Institute, Council of Agriculture Executive Yuan, Tainan, 71246, Taiwan
Phinyo, M.
Department of Agricultural Science, Faculty of Agriculture Natural Resources and Environment, Naresuan University, Phitsanulok, 65000, Thailand, The center for Agricultural Biotechnology, Naresuan University, Phitsanulok, 65000, Thailand
Tang, P. C.
Department of Animal Science, National Chung Hsing University, Taichung, 40227, Taiwan
Nfor, O. N.
Department of Public Health, Chung Shan Medical University, Taichung, 40227, Taiwan.

Abstract

The association between age and semen quality of Thai native roosters (Leung hang khao) in relation to patterns of seminal plasma proteins were determined. Eighteen of the native roosters were grouped according to the ages 7-8, 10-11 and 23-24 months. Semen was collected by abdominal massage and evaluated for different physical parameters. Results showed that semen volume, color, pH and sperm concentration were not significantly different among the different age groups. In contrast, sperm viability was found to be significantly different. Sperm viability was significantly higher in the 7-8 (93.34a ± 2.17) than the 23-24 months age category (71.38b ± 7.44). Seminal plasma proteins were separated using sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Based on our findings, proteins with molecular weight fractions of 72, 90, 140, 220, and 260 kDa showed patterns of expression in 7-8, 10-11, and 23-24 month old roosters, respectively. Moreover, proteins with molecular weight fractions of 72, 90, and 140 kDa were shown sharply patterns of expression among age of roosters. These results indicate that sperm vitality (percentage of live sperm or dead sperm) and proteins with molecular weight fractions of 90, and 140 kDa are associated with age. Proteins with molecular weight fractions of 90 and 140, kDa may serve as markers to determine semen quality in Thai native roosters.

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Inyawilert, W., Rungruangsak, J., Chanthi, S., Liao, Y. J., Phinyo, M., Tang, P. C., & Nfor, O. N. (2019). Age-related difference changes semen quality and seminal plasma protein patterns of Thai native rooster. International Journal of Agricultural Technology, 15(2), 287–296. retrieved from https://li04.tci-thaijo.org/index.php/IJAT/article/view/8209
Issue
Vol. 15 No. 2 (2019): March
Section
Original Study
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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